Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability a...Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA integrity.Methods:H4IIE-luc cells were incubated with different concentrations of AFB_(1) and/or FB_(1) for 24 and 48 h with or without aqueous extract of A.hybridus.Results:AFB_(1) decreased the viability of cells after 24 and 48 h of exposure.EC_(50)values for AFB_(1) were 10.5 and 1.8μmol/L for the two periods,respectively.When the 48 h exposure to mycotoxin repeated with a pre-treatment of 20 and 40μg/mL extract of A.hybridus,the EC_(50)changed to 3.88 and 7.67μmol/L,respectively.H4IIE-luc cells exposed to FB_(1) for 24 h responded more than those incubated for 48 h.Cells treated with a combination of AFB_(1) and FB_(1) were less viable with a signifi cant decrease in the greater concentration.The mixture of AFB_(1) and FB_(1) resulted in a signifi cant threat to H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis,which demonstrated damage to DNA.The protective effects were probably due to greater content of total phenolics,carotenoids,β-carotene,folic-,linolenic-,linoleic and palmitic acids,as well as calcium,magnesium,iron,zinc,and selenium observed in the extract.Conclusion:Exposure to 40μg/mL of extract of A.hybridus protected cells from damage to DNA by stabilizing DNA.展开更多
Aim:Afl atoxin B1(AFB1)and fumonisin B1(FB1)are important food-borne mycotoxins.Co-contamination of foodstuffs with these two mycotoxins is well-known and has been implicated in a possible development of hepatocellula...Aim:Afl atoxin B1(AFB1)and fumonisin B1(FB1)are important food-borne mycotoxins.Co-contamination of foodstuffs with these two mycotoxins is well-known and has been implicated in a possible development of hepatocellular carcinoma in humans living in regions of the world where exposures to these mycotoxins in grain are greatest.The aim of the current study was to evaluate the potential protective effects of an aqueous extract of Cochorus olitorius(C.olitorius,moroheiya)against cytotoxicity of AFB1 and/or FB1 in H4IIE-luc rat hepatoma cells,using assays to measure cell viability and disruption of DNA integrity.Although this transactivation assay was originally developed to specifi cally respond to aryl hydrocarbon agonists,this cell line was used because of its hepatic origin.Methods:H4IIE-luc cells were incubated with different concentrations of AFB1 and/or FB1 for 24 and 48 h with or without aqueous extract of C.olitorius.Results:Both mycotoxins decreased cell viability and increased DNA damage.Cytotoxicity was more pronounced when cells were exposed simultaneously to AFB1 and FB1.Conclusion:Aqueous extract of C.olitorius protected cells against cytotoxicity of mycotoxins.C.olitorius contains a water-soluble,natural chemo-preventative agent for cancer that should be isolated and identifi ed.展开更多
基金The Morogo Research Program gratefully acknowledges the National Research Foundation of South Africa(Focus Area Grant FA2004050600064)National Research Center,Cairo,Egypt Project No.10070112 for financial support of this study.Prof.Giesy was supported by the Canada Research Chair program,a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution,City University of Hong Kong,the 2012“High Level Foreign Experts”(No.GDT20143200016)program,funded by the State Administration of Foreign Experts Affairs,the P.R.China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences.
文摘Aim:Protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA integrity.Methods:H4IIE-luc cells were incubated with different concentrations of AFB_(1) and/or FB_(1) for 24 and 48 h with or without aqueous extract of A.hybridus.Results:AFB_(1) decreased the viability of cells after 24 and 48 h of exposure.EC_(50)values for AFB_(1) were 10.5 and 1.8μmol/L for the two periods,respectively.When the 48 h exposure to mycotoxin repeated with a pre-treatment of 20 and 40μg/mL extract of A.hybridus,the EC_(50)changed to 3.88 and 7.67μmol/L,respectively.H4IIE-luc cells exposed to FB_(1) for 24 h responded more than those incubated for 48 h.Cells treated with a combination of AFB_(1) and FB_(1) were less viable with a signifi cant decrease in the greater concentration.The mixture of AFB_(1) and FB_(1) resulted in a signifi cant threat to H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis,which demonstrated damage to DNA.The protective effects were probably due to greater content of total phenolics,carotenoids,β-carotene,folic-,linolenic-,linoleic and palmitic acids,as well as calcium,magnesium,iron,zinc,and selenium observed in the extract.Conclusion:Exposure to 40μg/mL of extract of A.hybridus protected cells from damage to DNA by stabilizing DNA.
基金The Morogo Research Program gratefully acknowledges the National Research Foundation of South Africa(Focus Area Grant FA2004050600064)National Research Center,Cairo,Egypt Project#10070112 for financial support of this study.Prof.Giesy was supported by the Canada Research Chair program,a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution,City University of Hong Kong,the 2012“High Level Foreign Experts”(#GDT20143200016)program,funded by the State Administration of Foreign Experts Affairs,the P.R.China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Scienc es.
文摘Aim:Afl atoxin B1(AFB1)and fumonisin B1(FB1)are important food-borne mycotoxins.Co-contamination of foodstuffs with these two mycotoxins is well-known and has been implicated in a possible development of hepatocellular carcinoma in humans living in regions of the world where exposures to these mycotoxins in grain are greatest.The aim of the current study was to evaluate the potential protective effects of an aqueous extract of Cochorus olitorius(C.olitorius,moroheiya)against cytotoxicity of AFB1 and/or FB1 in H4IIE-luc rat hepatoma cells,using assays to measure cell viability and disruption of DNA integrity.Although this transactivation assay was originally developed to specifi cally respond to aryl hydrocarbon agonists,this cell line was used because of its hepatic origin.Methods:H4IIE-luc cells were incubated with different concentrations of AFB1 and/or FB1 for 24 and 48 h with or without aqueous extract of C.olitorius.Results:Both mycotoxins decreased cell viability and increased DNA damage.Cytotoxicity was more pronounced when cells were exposed simultaneously to AFB1 and FB1.Conclusion:Aqueous extract of C.olitorius protected cells against cytotoxicity of mycotoxins.C.olitorius contains a water-soluble,natural chemo-preventative agent for cancer that should be isolated and identifi ed.
