Increased rates of ribosome biogenesis have been recognized as hallmarks of many cancers and are associated with poor prognosis.Using a CRISPR synergistic activation mediator(SAM)system library targeting 89 ribosomal ...Increased rates of ribosome biogenesis have been recognized as hallmarks of many cancers and are associated with poor prognosis.Using a CRISPR synergistic activation mediator(SAM)system library targeting 89 ribosomal proteins(RPs)to screen for the most oncogenic functional RPs in human esophageal squamous cell carcinoma(ESCC),we found that high expression of RPS15 correlates with malignant phenotype and poor prognosis of ESCC.展开更多
基金The authors are grateful to Dr.Zhi Lu(Tsinghua University,China)for kindly providing RNA-seq analysis and ribosome-seq analysis technique direction.The authors are grateful to Dr.Yutaka Shimada(Kyoto University,Japan)for the ESCC cell lines.The authors appreciate Cong Liu(Sinsage Technology Co.LTD)for providing the Livecyte single-cell tracking imaging analysis system.The authors thank Novogene Bioinformatics Technology Co.,Ltd.,Beijing,for assistance during the Ribosome-seq analysis.This work was supported by the National Natural Science Foundation of China(82188102,81903025,82273453 and 82030089)National Key R&D Program of China(2021YFC2501000 and 2020YFA0803300)+1 种基金CAMS Innovation Fund for Medical Sciences(2021-1-I2M-1-018,2022-I2M-2-001 and 2021-I2M-1-067)the Fundamental Research Funds for the Central Universities(3332021091).
文摘Increased rates of ribosome biogenesis have been recognized as hallmarks of many cancers and are associated with poor prognosis.Using a CRISPR synergistic activation mediator(SAM)system library targeting 89 ribosomal proteins(RPs)to screen for the most oncogenic functional RPs in human esophageal squamous cell carcinoma(ESCC),we found that high expression of RPS15 correlates with malignant phenotype and poor prognosis of ESCC.
