To assess, in vitro, the cellular viability in a murine macrophage cell line J774 with 9 different orthodontic wires and to evaluate the effects of its NO production. To assess cellular viability by MTT: 3-(4,5-dimeth...To assess, in vitro, the cellular viability in a murine macrophage cell line J774 with 9 different orthodontic wires and to evaluate the effects of its NO production. To assess cellular viability by MTT: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay in the cell line J774 with 9 different orthodontic wires and quantify NO production by these macrophages. Cell cultures were evaluated at 24, 48 and 72 hours. There was no significant difference of the means of cellular viability between the control and the group of wires in the respective time intervals. In the comparison with the control group, there was significant difference in the NO production in groups 1, 6, and 9 at 24 hours interval. Group 8 showed significant difference in relation to the control group at final time interval. Cellular viability in all groups was higher at the final time interval than at the initial time interval. This increase was significant in the control group. In the material groups, the final mean of cellular viability at 72 hours showed no significant difference when compared with the control group. NO production in all groups was higher at the final time interval than at the initial time interval. This increase was significant in the control group. In the material groups, the final mean of NO production at 72 hours was only significant in group 8 (betatitanium) when compared with the control group.展开更多
Comparing Cone-Beam Computed Tomography (CBCT) exams with different fields of view (FOV) are relevant to understand the impact of the inherent image quality on the reliability and accuracy of the diagnostic outcome. T...Comparing Cone-Beam Computed Tomography (CBCT) exams with different fields of view (FOV) are relevant to understand the impact of the inherent image quality on the reliability and accuracy of the diagnostic outcome. This study aimed to comparatively evaluate the results of the fractal analysis of images of the same structure taken in two cone beam computerized scanners with different FOVs. The initial selection of the images used, from two CBCT of the same individual jaw performed in two different scanners, was conducted in the Radiant program (RadiAnt DICOM Viewer) and exported to DICOM format. The selected images were processed in the ImageJ software (US National Institutes of Health), correcting the rotation of the images before conducting fractal analysis. In order to verify the normality of the data, the Shapiro-Wilk test was applied. The Mann-Whitney U, Levene and T tests were applied in order to compare the fractal analysis obtained by the two CBCT scanners. The intraclass correlation coefficient result was 0.988. The mean of the samples in the three comparisons (mentum samples, mental foramen samples and all included mentum and mental foramen samples) was higher for the PreXion 3D scanner. The p value of the tests was 0.000, for the three comparisons, at a 5% confidence level. The comparison of the fractal dimension averages of the images obtained with i-CAT and PreXion 3D scanners showed statistically significant differences, and higher averages were observed in the fractal analysis of images from PreXion 3D scanner. These results point to the need for a careful analysis of the parameters involved in the acquisition of images when results of fractal analysis resulting from different equipment are compared.展开更多
文摘To assess, in vitro, the cellular viability in a murine macrophage cell line J774 with 9 different orthodontic wires and to evaluate the effects of its NO production. To assess cellular viability by MTT: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay in the cell line J774 with 9 different orthodontic wires and quantify NO production by these macrophages. Cell cultures were evaluated at 24, 48 and 72 hours. There was no significant difference of the means of cellular viability between the control and the group of wires in the respective time intervals. In the comparison with the control group, there was significant difference in the NO production in groups 1, 6, and 9 at 24 hours interval. Group 8 showed significant difference in relation to the control group at final time interval. Cellular viability in all groups was higher at the final time interval than at the initial time interval. This increase was significant in the control group. In the material groups, the final mean of cellular viability at 72 hours showed no significant difference when compared with the control group. NO production in all groups was higher at the final time interval than at the initial time interval. This increase was significant in the control group. In the material groups, the final mean of NO production at 72 hours was only significant in group 8 (betatitanium) when compared with the control group.
文摘Comparing Cone-Beam Computed Tomography (CBCT) exams with different fields of view (FOV) are relevant to understand the impact of the inherent image quality on the reliability and accuracy of the diagnostic outcome. This study aimed to comparatively evaluate the results of the fractal analysis of images of the same structure taken in two cone beam computerized scanners with different FOVs. The initial selection of the images used, from two CBCT of the same individual jaw performed in two different scanners, was conducted in the Radiant program (RadiAnt DICOM Viewer) and exported to DICOM format. The selected images were processed in the ImageJ software (US National Institutes of Health), correcting the rotation of the images before conducting fractal analysis. In order to verify the normality of the data, the Shapiro-Wilk test was applied. The Mann-Whitney U, Levene and T tests were applied in order to compare the fractal analysis obtained by the two CBCT scanners. The intraclass correlation coefficient result was 0.988. The mean of the samples in the three comparisons (mentum samples, mental foramen samples and all included mentum and mental foramen samples) was higher for the PreXion 3D scanner. The p value of the tests was 0.000, for the three comparisons, at a 5% confidence level. The comparison of the fractal dimension averages of the images obtained with i-CAT and PreXion 3D scanners showed statistically significant differences, and higher averages were observed in the fractal analysis of images from PreXion 3D scanner. These results point to the need for a careful analysis of the parameters involved in the acquisition of images when results of fractal analysis resulting from different equipment are compared.
