时效处理对2195 Al-Li合金挤压型材腐蚀行为的影响

对2195铝锂合金分别进行欠时效(UA)、峰时效(PA)、双级时效(DA)和回归再时效(RRA)处理,观察并讨论其显微组织、晶间腐蚀(IGC)、剥落腐蚀(EXCO)和应力腐蚀开裂(SCC)行为。结果表明,合金经不同时效处理后,其IGC和EXCO耐蚀性顺序为:RRA>UA>DA>PA。PA和UA态的低耐蚀性主要是T_(1)相在晶界处连续析出形成连续的腐蚀通道引起的,而粗大不连续析出的T_(1)相以及较宽的无析出带导致的腐蚀通道中断是RRA态具有最佳耐蚀性的原因。RRA态合金表现出最好的抗应力腐蚀性能,抗应力腐蚀顺序为:RRA>DA>UA>PA。晶间T_(1)相的不连续析出促进了腐蚀裂纹穿过晶界进行扩展,从而提高了2195铝锂合金的抗应力腐蚀性能。

A Study on the Influence of Social Media Use on Psychological Anxiety among Young Women

To explore the relationship between social influence,social comparison,clarity of self-concept,and psychological anxiety among young women during their usage of social networking sites,our study selected 338 young women aged 14-34 from the social site platforms of Little Red Book and Weibo for questionnaire surveys.The Passive Social Network Utilization Scale,Social Comparison Scale(SCS),Social Influence Questionnaire,Self-Concept Clarity Scale(SCCS),and Generalized Anxiety Disorder Scale(GAD-7)were employed to measure the subjects.Our results show that the frequency of passive social media use is positively related to the level of psychological anxiety.Social comparison,social influence,and unclear self-concepts under social media use are negatively predictive of psychological anxiety.The chain mediation effects indicate that social comparison and social influence under social media use negatively predict the clarity of self-concept,thus having a negative impact on the psychological health of young women.Therefore,young women should strengthen their self-concepts,control their frequency of social media usage,avoid addiction,and pay special attention to their frequency of passive use,in order to protect their psychological health.Our study provides some practical implications and insights regarding the relationship between young women’s social media use and psychological health.

Study on Influencing Factors of Mental Health of Mobile Young White-Collar Workers in China

Mobile young white-collar workers not only have the characteristics of mobile young people,but also have the characteristics of general white-collar workers.Under the influence of both,their mental health may be suffering from“double disadvantage”.So,based on an ecological model of the stress process,this paper tries to use the data of the questionnaire on the mental health of mobile young white-collar workers in Zhejiang Province to explore the influence of some factors in the middle workplace and residence place on the mental health of micro individuals.The results show that:(1)The working environment with high control and low freedom and the workplace discrimination against the mobile status will have a negative impact on the mental health of mobile young white-collar workers;(2)Financial anxiety in daily life will lead to a decline in the mental health level of mobile young white-collar workers;(3)Good organizational support and neighborhood social relations can significantly relieve life pressure,so as to effectively improve the mental health of mobile young white-collar workers.It can be seen that we also need to pay more attention to the mental health of mobile young white-collar workers in order to improve their situation.

Adaptability of Development Methods for Offshore Gas Cap Edge Water Reservoirs under Different Permeability Levels

The BZ 34-1 oilfield is a typical gas cap edge water reservoir in the Bohai oilfield. The main characteristics of the oilfield were multi-phase sand body stacking and the sand body was composed of three parts: gas cap, oil reservoir, and edge water. The actual production site results show that the permeability difference of multi-layer sand bodies has a serious impact on the development effect. This article establishes a typical reservoir model numerical model based on the total recovery degree of the reservoir and the recovery degree of each layer, and analyzes the impact of permeability gradient. As the permeability gradient increases, the total recovery degree of all four well patterns decreases, and the total recovery degree gradually decreases. The recovery degree of low permeability layers gradually decreases, and the recovery degree of high permeability layers gradually increases. As the permeability gradient increases, the degree of recovery gradually decreases under different water contents. As the permeability gradient increases, the reduction rate of remaining oil saturation in low permeability layers is slower, while the reduction rate of remaining oil saturation in high permeability layers was faster. By analyzing the impact of permeability gradient on the development effect of oil fields, we could further deepen our understanding of gas cap edge water reservoirs and guide the development of this type of oil field.

均匀化处理对2195铝锂合金显微组织及再结晶行为的影响

采用扫描电镜、X射线衍射、透射电镜及电子背散射衍射等方法研究均匀化处理过程中2195合金的显微组织演变和Al3Zr析出相对再结晶行为的影响。结果表明,经均匀化处理后,铸态合金中严重的枝晶偏析得到有效消除,但其过程受到溶解速度较慢的Al−Cu相的限制。此外,与其他均匀化处理工艺(单级、双级和以60℃/h斜坡升温的均匀化处理)相比,在以30℃/h斜坡升温的均匀化处理条件下,样品(H30)中析出尺寸较细小且密度较大的Al3Zr弥散相。Al3Zr弥散相的尺寸越小,密度越高,其具有的Zener钉扎阻力越强,可阻碍位错及(亚)晶界的移动。因此,在热压缩及后续固溶处理过程中H30样品表现出较强的再结晶抗力。该研究能为铸态2195铝合金热处理工艺参数的优化提供理论指导。

乳腺髓样癌临床预测模型的建立和验证:基于SEER数据库

目的 基于美国监测,流行病学和最终结果(Surveillance,Epidemiology,and End Results,SEER)数据库,建立并验证预测乳腺髓样癌(medullary carcinoma of breast,MCB)患者5年和10年生存率的列线图模型。方法 从SEER数据库中收集2000年至2015年诊断为MCB的患者临床资料,将患者按7∶3的比例随机分配至训练集和验证集。通过单因素和多因素Cox回归分析确定MCB患者预后的独立危险因素,并构建列线图预测MCB患者的5年和10年生存率。采用一致性指数(C指数)、受试者工作特征曲线下面积(area under the receiver operating characteristic curve,AUC)、校准图和决策曲线分析(decision curve analysis,DCA),比较列线图模型和美国癌症联合委员会(The American Joint Committee on Cancer,AJCC)分期系统之间的预测性能。结果共纳入2 086例MCB患者,其中训练集1 460例、验证集626例。单因素和多因素Cox回归分析显示诊断年龄、婚姻状况、AJCC分期和手术是影响MCB患者生存的危险因素,将它们纳入列线图并进行验证,训练集中,列线图显示5年总体生存率AUC为0.698,10年总体生存率AUC为0.707;验证集中,列线图显示5年总体生存率AUC为0.748,10年总体生存率AUC为0.729。校准图显示列线图模型的预测结果与实际观察结果有较好的一致性,DCA结果显示列线图在训练集和验证集中产生了较好的净收益。结论 通过Cox回归分析构建了MCB患者5年和10年生存率的列线图预测模型,经验证列线图有较好的预测性能。该模型不仅为患者生存预后提供更加个性化的评估,也能为临床医生选择个性化治疗方案提供参考。

Intestinal flora and depressive disorders:exploration and prospect of microbial-gut-brain axis

This paper examines the correlation between depressive disorders and intestinal flora.Depression is a common affective disorder characterized by low mood,loss of interest,anhedonia,high incidence,high recurrence rate,high disability rate,and high medical costs.The incidence and harmfulness of depressive disorder are gradually increasing,and its etiology is complex and diverse,among which the abnormal intestinal flora is considered to be one of the causes of depressive disorder.This article reviews the results of several studies that found intestinal flora imbalance in depressed patients,including changes in the type and quantity of flora and changes in metabolic pathways.In addition,antibiotic and probiotic treatments have also been shown to be effective in alleviating depressive symptoms,further indicating the importance of intestinal flora disturbances in the pathogenesis of depression.We also explored the relationship between intestinal flora and the pathogenesis of depressive disorders.Through neuro-immuno-endo-crine-metabolic pathways,intestinal flora can affect the function of the central nervous system,cause changes in the host’s mental behavior,and lead to or aggravate depressive symptoms.Overall,this study not only found differences in the intestinal flora of patients with depressive disorders but also revealed the potential role of intestinal flora in the pathogenesis.Importantly,this provides a new theoretical basis for further clarifying the pathogenesis of depressive disorders and formulating diagnosis and treatment strategies.

The Efficient Tissue Culture System of Orostachys fimbriata

This study aimed to investigate the tissue culture and rapid propagation techniques of Orostachys fimbriata, a medicinal and ornamental herbaceous perennial herb belonging to the family Crassulaceae. The leaves of O. fimbriata were used as explants to investigate the effects of plant growth regulators such as thidiazuron (TDZ), 6-benzylamino purine (6-BA) and 1-naphthaleneacetic acid (NAA), on the induction of callus, differentiation of adventitious buds and rooting of shoots. Our results showed that optimum callus induction medium was MS medium supplemented with 0.5 mg·L-1 TDZ and 0.2 mg·L-1 NAA. 1.5 mg·L-1 6-BA and 0.2 mg·L-1 NAA was the optimum hormone combination for differentiation of adventitious buds. And 0.1 mg·L-1 NAA could efficiently promote rooting. The survival rate of transplants reached about 90%. In this study, using leaves of O. fimbriata as explants, high efficient tissue culture and regeneration system of O. fimbriata were established, and the period from leave to transplant plantlet was about 3 months. The presently developed protocol could be used for large scale clonal propagation and germplasm conservation of O. fimbriata. The efficient tissue culture system of O. fimbriata would provide technical support for its utilization.

留学生血液学英语教学的探索与实践

随着"一带一路"国策的提出,相关国家来华医学留学生教育规模逐年扩大。这些学生以英语授课,培养学历人才为主。如何提高医学教育质量是我们面临的紧迫任务。我们总结本校对外国留学生进行血液学英语教学的经验和体会,旨在探索提高医学留学生血液学临床教学质量的方法和途径。

Biodistribution and Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles In Vitro and In Vivo

Biodistribution and toxicity assessment are critical for safe clinical use of newly developed medicines.Superparamagnetic iron oxide nanoparticles (SPION)are effective carriers for targeted drug delivery.This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI)(SPION-PEI)designed for small interfering RNA (siRNA) delivery both in vitro and in vivo.SPION-PEI/siRNA complexes were prepared at different weight ratios.Cytotoxic effects of SPION-PEI/siRNA on HSC-T6 cell viability were determined by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT).Rats were divided into three groups:a control group,a normal-saline group and a SPION-PEI/siRNA group.After a single intravenous injection,in vivo nanoparticle biodistribution and accumulation were evaluated by Prussian blue staining in the heart,liver,spleen,lung and kidney 8 h,24 h,and 7 days after the injection.Their distribution was histologically studied at the three time points by measuring ironpositive areas (μm2)in organ sections stained with Prussian blue.The same organs were analyzed by H&E staining for any possible histopathological changes.Furthermore,biochemical indexes such as alanine amino transaminase (ALT),aspartate transaminase (AST),blood urea nitrogen (BUN)and creatinine (CREA)were also assessed at all experimental time points.Electrophoresis exhibited that the SPION-PEI could retard siRNA altogether at weight ratios above 4.MTT assay showed that SPION-PEI loaded with siRNA had low cytotoxicity.In vivo study revealed that the liver and spleen were the major sites of SPION-PEI/siRNA deposition.The iron content was significantly increased in the liver and spleen,peaking 24 h after intravenous injection and then declining gradually.No evidence was found of irreversible histopathological damage to any of the organs tested.These results suggested that most SPION-PEI/siRNA complexes were distributed in the liver and spleen,which might be the target organs of SPION-PEI/siRNA complexes.SPION- PEI/siRNA may serve as in vivo carrier for biomedical medicines.

Complement C3a activates osteoclasts by regulating the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma

Objective:Myeloma bone disease(MBD)is the most common complication of multiple myeloma(MM).Our previous study showed that the serum levels of C3/C4 in MM patients were significantly positively correlated with the severity of bone disease.However,the mechanism of C3 a/C4 a in osteoclasts MM patients remains unclear.Methods:The formation and function of osteoclasts were analyzed after adding C3 a/C4 a in vitro.RNA-seq analysis was used to screen the potential pathways affecting osteoclasts,and the results were verified by Western blot,q RT-PCR,and pathway inhibitors.Results:The osteoclast area per view induced by 1μg/m L(mean±SD:50.828±12.984%)and 10μg/m L(53.663±12.685%)of C3 a was significantly increased compared to the control group(0μg/m L)(34.635±8.916%)(P<0.001 and P<0.001,respectively).The relative m RNA expressions of genes,OSCAR/TRAP/RANKL/cathepsin K,induced by 1μg/m L(median:5.041,3.726,1.638,and 4.752,respectively)and 10μg/m L(median:5.140,3.702,2.250,and 5.172,respectively)of C3 a was significantly increased compared to the control group(median:3.137,2.004,0.573,and 2.257,respectively)(1μg/m L P=0.001,P=0.003,P<0.001,and P=0.008,respectively;10μg/m L:P<0.001,P=0.019,P<0.001,and P=0.002,respectively).The absorption areas of the osteoclast resorption pits per view induced by 1μg/m L(mean±SD:51.464±11.983%)and 10μg/m L(50.219±12.067%)of C3 a was also significantly increased(33.845±8.331%)(P<0.001 and P<0.001,respectively)compared to the control.There was no difference between the C4 a and control groups.RNA-seq analysis showed that C3 a promoted the proliferation of osteoclasts using the phosphoinositide 3-kinase(PI3 K)signaling pathway.The relative expressions of PIK3 CA/phosphoinositide dependent kinase-1(PDK1)/serum and glucocorticoid inducible protein kinases(SGK3)genes and PI3 K/PDK1/p-SGK3 protein in the C3 a group were significantly higher than in the control group.The activation role of C3 a in osteoclasts of MM patients was reduced by the SGK inhibitor(EMD638683).Conclusions:C3 a activated osteoclasts by regulating the PI3 K/PDK1/SGK3 pathways in MM patients,which was reduced using a SGK inhibitor.Overall,our results identified potential therapeutic targets and strategies for MBD patients。

Effects of foraging site distances on the intestinal bacterial community compositions of the sympatric wintering Hooded Crane(Grus monacha)and Domestic Duck(Anas platyrhynchos domesticus)

Background:The composition of intestinal microflora in animals is affected by cross-species transmission.In a nature reserve,the foraging sites of waterbirds are relatively fixed,but frequently close to residential areas and can also be visited by domestic fowls.It is easy to result in the trans-species-flock dispersal of gut microbes between the wild birds and domestic fowls.The effects of the variable foraging site distances on the gut microbe structures of the waterbirds and the sympatric domestic fowls are currently unclear,and further research is required to evaluate the impacts of geographic location on cross-infection.Methods:Illumina high-throughput sequencing and bioinformatics analysis software were utilized to compare and analyze the composition of gut microbes from the fecal samples of Hooded Cranes(HC;Grus monacha)and two groups of Domestic Ducks(Anas platyrhynchos domesticus)that foraged at 1 km(ducks in near areas,D-N),and 4 km(ducks in far areas,D-F)away from the habitats of the Hooded Cranes at Shengjin Lake,China.Results:The results showed that there were significant differences in the alpha-diversity of the gut bacteria in the HC,D-N,and D-F samples under the interspecific distance factor.The dominant bacterial phyla,Cyanobacteria and Proteobacteria,showed correlations with distance for each host.The D-N group had more diverse intestinal flora than the D-F,as they were physically closer to the HC and had more indirect contact and cross-transmission of their gut microbes.More potentially pathogenic bacterial sequences,and Operational Taxonomic Units(OTUs)were found in the D-N than in HC and D-F.Conclusions:Hooded Cranes and the Domestic Duck populations at variable distances from the cranes showed significant differences in their intestinal bacteria and potentially pathogenic bacteria.The closer the foraging sites were,the easier the intestinal flora spread across species.The results provide a basis for determining the safe distance between wild birds and domestic fowls in a nature reserve.

SUBTYPES OF B LYMPHOCYTES IN PATIENTS WITH AUTOIMMUNE HEMOCYTOPENIA

Objective To investigate the quantities of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia and the relationship between quantities of CD5+ B lymphocytes and clinical or laboratorial parameters. Methods Quantities of CD5+ B lymphocytes in the bone marrow of 14 patients with autoimmune hemolytic anemia (AIHA) or Evans syndrome, 22 immunorelated pancytopenia (IRP) patients, and 10 normal controls were assayed by flow cytometry. The correlation between their clinical or laboratorial parameters and CD5+ B lymphocytes was analyzed. Results The quantity of CD5+ B lymphocytes of AIHA/Evans syndrome (34.64%±19.81%) or IRP patients (35.81%±16.83%) was significantly higher than that of normal controls (12.00%±1.97%, P<0.05). However, there was no significant difference between AIHA/Evans syndrome and IRP patients (P>0.05). In all hemocytopenic patients, the quantity of bone marrow CD5+ B lymphocytes showed significantly negative correlation with serum complement C3 level (r=-0.416, P<0.05). In the patients with AIHA/Evans syndrome, the quantity of bone marrow CD5+ B lymphocytes showed significantly positive correlation with serum indirect bilirubin level (r=1.00, P<0.05). In Evans syndrome patients, the quantity of CD5+ B lymphocytes in bone marrow showed significantly positive correlation with platelet-associated immunoglobulin G (r=0.761, P<0.05) and platelet-associated immunoglobulin M (r=0.925, P<0.05). The quantity of CD5+ B lymphocytes in bone marrow of all hemocytopenic patients showed significantly negative correlation with treatment response (tau-b=-0.289, P<0.05), but had no correlation with colony forming unit-erythroid (r=-0.205, P>0.05) or colony forming unit-granulocyte-macrophage colonies (r=-0.214, P>0.05). Conclusions The quantity of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia significantly increases and is correlated with disease severity and clinical response, which suggest that CD5+ B lymphocytes might play an important role in the pathogenesis of autoimmune hemocytopenia.

Comparing the intestinal bacterial communies of sympatric wintering Hooded Crane (Grus monacha) and Domestic Goose(Anser anser domesticus)

Background:Gut microbiota play crucial roles in host health.Wild birds and domestic poultry often occupy sympatric habitats,which facilitate the mutual transmission of intestinal microbes.However,the distinct intestinal microbial communities between sympatric wild birds and poultry remain unknown.At present,the risk of interspecies transmission of pathogenic bacteria between wild and domestic host birds is also a research hotspot.Methods:This study compared the intestinal bacterial communities of the overwintering Hooded Crane(Grus monacha)and the Domestic Goose(Anser anser domesticus)at Shengjin Lake,China,using Illumina high-throughput sequencing technology(Mi-Seq platform).Results:Our results revealed that Firmicutes,Actinobacteria,Proteobacteria,Bacteroidetes and Chloroflexi were the dominant bacterial phyla in both hosts.The gut bacterial community composition differed significantly between sympatric Hooded Cranes and Domestic Geese.However,the hosts exhibited little variation in gut bacterial alphadiversity.The relative abundance of Firmicutes was significantly higher in the guts of the Hooded Cranes,while the relative abundances of Actinobacteria,Proteobacteria,Bacteroidete and Chloroflexi were significantly higher in guts of Domestic Geese.Moreover,a total of 132 potential pathogenic operational taxonomic units(OTUs)were detected in guts of Hooded Cranes and Domestic Geese,and 13 pathogenic OTUs(9.8%)were found in both host guts.Pathogenic bacterial community composition and diversity differed significantly between hosts.Conclusions:The results showed that the gut bacterial community composition differs significantly between sympatric Hooded Cranes and Domestic Geese.In addition,potential pathogens were detected in the guts of both Hooded Cranes and Domestic Geese,with 13 pathogenic OTUs overlapping between the two hosts,suggesting that more attention should be paid to wild birds and poultry that might increase the risk of disease transmission in conspecifics and other mixed species.

左心室壁运动异常与缺血性卒中患者卒中复发的关系

目的探讨左心室壁运动异常(LVWMA)与缺血性卒中患者卒中复发的相关性。方法采用前瞻性队列研究共纳入急性缺血性卒中患者864例,将其分为LVWMA阳性组和阴性组。患者出院后进行随访,分析并比较患者卒中复发情况。结果两组患者在性别、年龄、高血压、糖尿病、冠状动脉粥样硬化性心脏病(冠心病)、心律失常、心脏瓣膜病、左心室射血分数<30%发生率,C反应蛋白、三酰甘油、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、N末端脑钠肽、服用抗血小板药物和抗凝血药及急性卒中Org10172治疗试验分型比较,差异有统计学意义(P<0.05);两组患者随访12、24个月卒中的发生率比较,差异有统计学意义(P <0.05)。单因素Logistic回归分析显示,LVWMA阳性患者缺血性卒中复发风险是阴性者的2.61倍,年龄、吸烟、高血压、糖尿病、冠心病、总胆固醇、低密度脂蛋白胆固醇、空腹血糖、N末端脑钠肽、出院时服用抗凝血药、心源性脑栓塞性卒中及大动脉粥样硬化性卒中为缺血性卒中者卒中复发的危险因素(P <0.05)。调整各因素后LVWMA阳性者卒中复发风险是阴性者的2.97倍。结论 LVWMA可能引起左室血栓形成,是缺血性卒中复发的独立危险因素。

TET2 Expression in Bone Marrow Mononuclear Cells of Patients with Myelodysplastic Syndromes and Its Clinical Significances

Objective To investigate the expression of TET2 mRNA and protein in the bone marrow mononuclear cells(BMMNC) of patients with myelodysplastic syndrome(MDS) and its clinical significance. Methods The expression of TET2 mRNA and protein in bone marrow mononuclear cells(BMMNC) of 32 patients with MDS and 20 healthy donors was examined by qPCR and Western blot. Results The expression of TET2 mRNA in BMMNC was down-regulated in MDS patients compared with the donor group [(0.41±0.28)%vs.(1.07±0.56)%](P<0.001).Compared with lower expression group(TET2<0.4)[(6.53±6.17)%],patients with higher expression of TET2(≥0.4) presented significantly lower proportion of bone marrow blasts[(1.21±1.56)%](P<0.05).The expression of TET2 mRNA in BMMNC of MDS patients was inversely correlated with malignant clone burden(r=-0.398,P<0.05) and IPSS(r=-0.412, P<0.05).The expression of TET2 protein was down-regulated in MDS patients compared with that in the donor group. Conclusions The mRNA and protein expression of TET2 in BMMNC of MDS patients is decreased,which might be useful as an important parameter for the evaluation of MDS clone burden.

探讨内科胸腔镜与胸水癌胚抗原检测在不明原因胸腔积液中的诊断价值

目的探讨内科胸腔镜下胸膜活检术和胸水癌胚抗原(CEA)对不明原因胸腔积液的诊断价值。方法 160例不明原因胸腔积液患者,胸腔镜术前行胸穿穿刺抽液术,完善胸水常规、生化及胸水CEA检查,随后完善内科胸腔镜行胸膜活检送病理。观察胸腔镜下的胸膜表现及良恶性胸腔积液的特征,判定胸水CEA对诊断恶性胸腔积液的敏感性、特异性、阳性预测值及阴性预测值。结果非恶性胸腔积液122例,其中结核性胸膜炎102例,炎症性胸腔积液20例(脓胸4例,肺炎旁胸腔积液16例);恶性胸腔积液38例,其中鳞癌2例,腺癌35例,恶性淋巴瘤1例。胸腔积液患者均完善胸水CEA检测,恶性胸腔积液患者的CEA水平为105.95(16.60,903.75)μg/ml,非恶性胸腔积液患者的CEA水平为1.60(1.10,2.70)μg/ml,恶性胸腔积液患者的CEA水平明显高于非恶性胸腔积液,差异有统计学意义(P<0.05)。胸水CEA诊断恶性胸腔积液的敏感性、特异性、阳性预测值(PPV)、阴性预测值(NPV)分别为86.8%、86.9%、67.3%、95.5%。结论胸水CEA对恶性胸腔积液诊断有良好的特异性和敏感性,内科胸腔镜在不明原因胸腔积液诊断方面有着方便、快捷、安全的特点,二者联合对恶性胸腔积液的诊断有一定的价值。

波浪作用下渗透率各向异性的海床液化分析

为探究波浪荷载作用下渗透率各向异性的海床的瞬态液化问题,分别以雷诺平均Navier-Stokes(RANS)方程和Biot多孔弹性方程作为波浪运动和海床响应的控制方程,采用LSM (LevelSet Method)法对自由表面进行追踪,以保证波浪运动模拟的准确性,并建立了波浪-海床相互作用的二维耦合数值模型.在验证数值模型合理性的基础上,进一步分析了波浪参数、海床土体的饱和度和渗透性对海床瞬态液化的影响.结果表明:波浪参数和海床土体饱和度对海床瞬态液化的影响显著;海床的瞬态最大液化深度随着波浪的高度、周期的增大而增加,随着海床土体饱和度的增大而减小;相比于海床土体的水平方向渗透系数,海床的瞬态最大液化深度对垂直方向渗透系数的变化更加敏感.

Expression of DLK1 Gene in the Bone Marrow Cells of Patients with Myelodysplastic Syndromes and Its Clinical Significance

Objective This study aims to investigate the expression of delta-like 1(DLKl) gene in the bone marrow cells of patients with myelodysplastic syndromes(MDS) and to explore its molecular characteristics for the early diagnosis of MDS. Methods The expression of DLK1 mRNA in the bone marrow cells of cases with MDS,acute myeloid leukemia(AML),and normal control groups were measured by real-time polymerase chain reaction and were analyzed for clinical significance. Results Significantly higher expression of DLK1 mRNA was observed in the bone marrow cells of MDS patients(0.7342±0.3652) compared with the normal control group(0.4801±0.1759)(P<0.05).The expression of DLK1 mRNA had a positive correlation with the proportion of bone marrow blasts(r=0.467,P<0.05).Moreover,DLKl mRNA expression was significantly increased as MDS progressed (P<0.05).Patients with abnormal karyotypes exhibited significantly higher expression of DLKl mRNA(0.9007±0.4334) than those with normal karyotypes(0.6411±0.2630)(P<0.05).Subsequently,patients with highly expressed DLKl(>0.8) presented significantly higher malignant clone burden(0.4134±0.3999) than those with lower DLKl expression(<0.8),(0.1517±0.3109),(P<0.05). Conclusions The DLK1 gene was highly expressed in MDS patients,and was increased as MDS progressed.The expression of DLK1 mRNA was positively correlated with the proportion of the bone marrow blasts.A high expression of DLKl gene suggested a higher malignant clone burden of MDS.

Diagnosis of upper gastrointestinal perforation complicated with fistula formation and subphrenic abscess by contrast-enhanced ultrasound:A case report

BACKGROUND Gastrointestinal perforation complicated by subphrenic abscess is a surgical emergency.Its diagnosis relies mainly on X-ray or computed tomography(CT),while the value of ultrasound,especially contrast-enhanced ultrasound(CEUS),has been underestimated.CASE SUMMARY A 37-year-old man presented with fever and edema of the lower extremities for 10 d.He had a history of laparoscopic repair of gastroduodenal perforation 6 mo prior.His first-time intravenous CEUS indicated a diagnosis of subphrenic abscess.He received antibiotic therapy and ultrasound-guided percutaneous drainage of the abscess.However,second-time intravenous CEUS revealed an unsatisfactory therapeutic effect.Intracavitary CEUS was proposed,and this examination detected communication between the abscess and the stomach.Upper gastrointestinal perforation complicated by fistula formation and subphrenic abscess was diagnosed with the help of CEUS.Abdominal CT and esophagogastroduodenoscopy confirmed the diagnosis.The patient recovered after the perforation was repaired by surgery.CONCLUSION Intravenous and intracavitary CEUS provides helpful information for the diagnosis of upper gastrointestinal perforation complicated by fistula formation and subphrenic abscess.