播种量与壮秧剂对湘南机插晚稻秧苗素质及产量的影响

提高秧苗素质是提高水稻产量与抗逆性的有效途径。为明确播种量和壮秧剂喷施时期对湘南机插晚稻秧苗素质和产量的影响,以H优518为材料,设置3个壮秧剂喷施时期(A1.1叶1心期;A2.2叶1心期;A3.1叶1心期+2叶1心期)与4个播种量(干种子)(B1.75 g/盘;B2.90 g/盘;B3.105 g/盘;B4.120 g/盘),于2018—2019年在湖南衡阳县开展大田试验。结果表明:(1)随播种量增加,秧苗成苗数和苗高增加,但成苗率降低,每穴总根数、平均根长、茎基宽和百株干质量减少;壮秧剂喷施时期中以A1和A2处理秧苗素质较高。(2)随播种量增加,秧苗分蘖速度逐渐降低,最高茎蘖数和有效穗数逐渐提高,壮秧剂喷施时期对水稻分蘖无显著影响;播种量和壮秧剂喷施时期对晚稻干物质积累和LAI影响显著,以A1B2和A2B2处理较高。(3)晚稻产量以B2处理最高,较B1、B3和B4分别提高7.1%、14.4%、9.9%;壮秧剂喷施时期中以A1处理产量较高;两者互作中以A1B2和A2B2处理产量较高。(4)相关性分析表明,产量与成苗率、每株总根数、干物质积累量、每穗粒数和结实率呈显著正相关。综上所述,湘南机插晚稻在播种量为90 g/盘、1叶1心期或2叶1心期喷施多效唑条件下,可改善秧苗素质,促进物质积累,提高每穗粒数和结实率,进而获得最佳增产效果。

Research on Identification Method of Apple Diseases in Southern Xinjiang Based on Deep Learning and Its System Implementation

Apple disease samples were collected from the southern Xinjiang and annotated to design a convolutional neural network model based on deep learning.The accuracy and robustness of the model was improved through training and optimization algorithms,and a complete apple disease identification system was developed with the model as the core,and evaluated for its performance in terms of accuracy,recall rate and speed.This study provides a reliable AI-based apple disease diagnosis solution for the apple planting industry in the southern Xinjiang,hoping to help farmers better manage and protect crop health.

Integrated Green Prevention and Control Techniques for Kiwifruit Canker Disease in "Guichang" Kiwifruit in Xiuwen County, Guizhou Province

Kiwifruit canker disease seriously affects the yield and quality of"Guichang"kiwifruit in Xiuwen County,Guizhou Province.In order to scientifically,safely,greenly and efficiently prevent and control the disease,theory was combined with prevention and control techniques to optimize existing prevention and control techniques,so as to improve the production yield and quality of kiwifruit.Specifically,biocontrol strains targeting local kiwifruit canker disease were screened,and reduced and mixed use of agrochemicals with improved efficiency was studied;and the effects and application techniques of disease resistance inducers and bioorganic fertilizers in inducing systemic disease resistance in kiwifruit trees were explored,and finally,an integrated green prevention and control scheme for kiwifruit canker disease that is suitable for kiwifruit production areas in Guizhou Province and has strong operability was proposed.This study provides technical support for green,efficient,standardized production technical services and sustainable and healthy development of kiwifruit industry.

显微镜结肠炎临床特征及其相关危险因素分析

目的分析慢性腹泻患者的临床、内镜及病理资料,探讨可能影响显微镜结肠炎(MC)的危险因素。方法前瞻性分析2016年6月-2017年4月该院门诊以水样腹泻为主诉患者的临床资料,常规结肠镜检查,多点取肠黏膜活检,行组织病理HE染色和Masson染色,对患者的病史及病理进行分析。结果 102例水样腹泻患者中诊断为MC 3例(3/102,2.94%),其中胶原性结肠炎(CC)3例,全部为女性,年龄24~36岁,淋巴细胞性结肠炎(LC)0例。结肠镜下肠黏膜基本正常或仅表现为轻度的充血水肿;病理组织学表现为上皮细胞损伤,上皮内淋巴细胞增多,固有层炎症细胞浸润,上皮下胶原带增厚。近期服用降糖药、质子泵抑制剂(PPIs)、面食等因素可能与MC发病关系密切。结论 MC是慢性水样腹泻的重要原因,临床症状及结肠镜表现均无特异性,慢性腹泻患者应尽早行结肠镜下多点活检明确诊断,其发病可能与使用某些药物(如降糖药、PPIs)和某些食物(面筋)有关。

氯沙坦通过AT1R/VEGF信号通路对肝癌血管生成的影响

目的探讨氯沙坦是否通过下调血管紧张素Ⅱ1型受体(AT1R)/血管内皮生长因子(VEGF)信号通路,抑制肝癌细胞的血管生成。方法采用免疫荧光染色和Western blot检测AT1R在肝癌细胞系Hep G2、Hu H-7和PLC/PRF/5,以及正常肝细胞LO2中的表达。采用Western blot检测氯沙坦对肝癌细胞系Hep G2中AT1R表达的影响,酶联免疫吸附法(ELISA)检测氯沙坦对肝癌细胞系Hep G2中VEGF的影响。采用免疫组织化学法检测氯沙坦对大鼠肝癌组织中AT1R、VEGF和CD34表达的影响。结果AT1R在正常肝细胞LO2中低表达(P<0.05),在肝癌细胞系Hep G2、Hu H-7和PLC/PRF/5中高表达(P<0.05),在Hep G2细胞中表达最高(P<0.05)。在肝癌细胞系Hep G2中加入氯沙坦后,AT1R蛋白水平和VEGF浓度降低(P<0.05)。大鼠肝癌组织中AT1R、VEGF和CD34高表达,而加入氯沙坦后,AT1R、VEGF和CD34表达降低(P<0.05)。结论氯沙坦通过抑制AT1R/VEGF信号通路,可以阻碍肝癌血管的生成。

糖尿病性角膜病变发病机制的研究进展

糖尿病性角膜病变(diabetic keratopathy,DK)是糖尿病在眼部的常见并发症之一,其主要临床表现包括干眼症、点状角膜炎、角膜上皮再生迟缓、反复的上皮糜烂、角膜知觉减退和角膜水肿等,主要发病机制为糖基化反应、多元醇通路和蛋白酶改变等。本文就糖尿病角膜病变的主要临床表现相对应的发病机制进行综述,以期对临床治疗提供新思路。

肠道菌群与microRNA失调对慢传输型便秘的影响

慢传输型便秘是一种常见的临床疾病,患者常伴有肠道菌群的改变。肠道菌群对维持人体健康有重要作用,若肠道菌群的结构和功能发生变化,出现肠道菌群紊乱,则可能引起便秘等疾病。MicroRNA是一种小的非编码RNA,作为基因表达过程转录后调控因子,其具有调节包括胃肠道在内的所有细胞蛋白表达的作用。肠道菌群能通过microRNA调节宿主基因的表达,而宿主的microRNA同样能调节菌群的生长和基因表达。肠道菌群与microRNA在维持肠道内稳态和预防相关疾病方面发挥着重要作用。该文就肠道菌群与宿主microRNA对慢传输型便秘影响的研究进展进行综述。

Characterization of mechanical properties of aluminum cast alloy at elevated temperature

The tensile response, the low cycle fatigue(LCF) resistance, and the creep behavior of an aluminum(Al) cast alloy are studied at ambient and elevated temperatures.A non-contact real-time optical extensometer based on the digital image correlation(DIC)is developed to achieve strain measurements without damage to the specimen. The optical extensometer is validated and used to monitor dynamic strains during the mechanical experiments. Results show that Young's modulus of the cast alloy decreases with the increasing temperature, and the percentage elongation to fracture at 100℃ is the lowest over the temperature range evaluated from 25℃ to 300℃. In the LCF test, the fatigue strength coefficient decreases, whereas the fatigue strength exponent increases with the rising temperature. The fatigue ductility coefficient and exponent reach maximum values at 100℃. As expected, the resistance to creep decreases with the increasing temperature and changes from 200℃ to 300℃.

Two-dimensional multimetallic sulfide nanosheets with multi-active sites to enhance polysulfide redox reactions in liquid Li2S6-based lithium-polysulfide batteries

The lithium-sulfur battery has attracted enormous attention as being one of the most significant energy storage technologies due to its high energy density and cost-effectiveness.However,the "shuttle effect" of polysulfide intermediates represents a formidable challenge towards its wide applications.Herein,we have designed and synthesized two-dimensional Cu,Zn and Sn-based multimetallic sulfide nanosheets to construct multi-active sites for the immobilization and entrapment of polysulfides with offering better performance in liquid Li2S6-based lithium-polysulfide batteries.Both experimental measurements and theoretical computations demonstrate that the interfacial multi-active sites of multimetallic sulfides not only accelerate the multi-chained redox reactions of highly diffusible polysulfides,but also strengthen affinities toward polysulfides.By adopting multimetallic sulfide nanosheets as the sulfur host,the liquid Li2 S6-based cell exhibits an impressive rate capability with 1200 mAh/g and retains 580 mAh/g at 0.5 mA/cm^(2) after 1000 cycles.With high sulfur mass loading conditions,the cell with 2.0 mg/cm^(2) sulfur loading delivers a cell capacity of 1068 mAh/g and maintains 480 mAh/g with 0.8 mA/cm^(2) and 500 cycles.This study provides new insights into the multifunctional material design with multi-active sites for elevated lithium-polysulfide batteries.

Searchable Encryption Cloud Storage with Dynamic Data Update to Support Efficient Policy Hiding

Ciphertext policy attribute based encryption(CP-ABE)can provide high finegrained access control for cloud storage.However,it needs to solve problems such as property privacy protection,ciphertext search and data update in the application process.Therefore,based on CP-ABE scheme,this paper proposes a dynamically updatable searchable encryption cloud storage(DUSECS)scheme.Using the characteristics of homomorphic encryption,the encrypted data is compared to achieve efficient hiding policy.Meanwhile,adopting linked list structure,the DUSECS scheme realizes the dynamic data update and integrity detection,and the search encryption against keyword guessing attacks is achieved by combining homomorphic encryption with aggregation algorithm.The analysis of security and performance shows that the scheme is secure and efficient.

Anti-tumor effect of LTA combined with 5-FU on H22 tumor bearing mice

Objective: To study the effect of lipoteichoic acid(LTA) and 5-FU on the expression of caspase-3, EGFR, TGF-α proteins of tumor tissue of H22 cancer bearing mice and its antitumor mechanism. Methods: A total of 40 SPF grade Kunming mice were selected to establish H22 liver cancer model, and then the mice were divided into 4 groups at random with ten mice in each group. Group A was given saline lavage treatment, Group B was treated with 5-FU by intraperitomeal injection, Group C was treated with LTA by lump body injection; Group D was treated with LTA by lump body injection and 5-FU by intraperitomeal injection. Two weeks after the treatment, the mice in each group were executed and the tumor tissue was stripping and weighted, and the tumor growth inhibition ratio was calculated. Then the tumor tissue was processed for conventional embedding, sectioned to observe the expression of caspase-3, EGFR, TGF-α by immunohistochemical staining method. Results: The tumor inhibitory rate o f Group D was significantly higher than Groups B and C(P<0.05); B, the tumor inhibitory rate o f Group B had no statistical difference compared with Group C(P>0.05). The IDO values of TGF-α, EGFR proteins in Groups B, C, D mice tumor tissue were significantly lower than that in group A(P<0.05); while IDO value of caspase-3 in Groups B, C, D group mice tumor tissue was significantly higher than that in Group A(P<0.05). The IDO value of TGF-α, EGFR in Group D mice tumor tissue were significantly lower than that in Groups B and C; While IDO value of aspase-3 in Group D was significantly higher than that in Groups B and C(P<0.05). Conclusions: LTA combined with 5-FU can effectively inhibit the tumorigenesis of H22 tumor bearing mice, increase the caspase-3 protein expression, inhibit TGF –α and EGFR protein expression, further promote tumor cell apoptosis and play a synergistic antitumor effect.

Change of the peripheral blood immune pattern and its correlation with prognosis in patients with liver cancer treated by sorafenib

Objective:To study the change of the peripheral blood immune pattern and its correlation with prognosis in patients with liver cancer after treated by sorafenib.Methods:Patients with advanced liver cancer admitted in our hospital were enrolled and treated with sorafenib.After two months of the treatment,their peripheral blood was collected.The immune cell subset and cytokines level were determined by flow cytometry and luminex technology.According to the reaction expressed by patients towards sorafenib,patients were divided into the response group and the no response group.The changes of the peripheral blood immune pattern and its correlation with prognosis of patients in the two groups were compared.Results:Before and after treatment of sorafenib,there was no significant difference in the ratios of T cells,NK cells and their subtypes in peripheral blood of patients between the two groups;while after treatment the ratio of B cells and regulatory B cells(Breg) of patients in the response group was significant higher than that of the no response group(P<0.05),and the prognosis conditions of patients with decreased ratio of Breg cells were better than other patients after undergoing chemotherapy.The levels of plasma cytokines IL-6,IL-10,IL-12,IL17,FIL-3L,IFN- γ,TNF-α,MCP-1 and VEGF showed no significant differences.Conclusions:After treatment of sorafenib,the prognosis conditions of patients of advanced liver cancer with a reduced Breg ratio are better than patients with an unaltered or increased Breg ratio.The ratio of Breg in peripheral blood may be considered as early biological indicator for the prediction of the curative effects of sorafenib.

Efficacy of the WINROP algorithm for retinopathy of prematurity screening in Southern China

AIM:To evaluate the predicting efficacy of severe retinopathy of prematurity(ROP)by the WINROP algorithm(http://winrop.com)in Southern China.METHODS:All preterm infants with the gestational age(GA)less than 32 wk were included.Their ROP screening results and serial postnatal body weight were analysed retrospectively.Weekly body weight was entered into and measured by the WINROP system.The outcomes were analysed,and the sensitivity,specificity,positive predictive value and negative predictive value(NPV)were calculated.RESULTS:Totally 432 infants with a median GA of 30.0(24.0-31.9)wk,and a median birth weight(BW)of 1360(540-2700)g were included.Among these 432 infants,50 were diagnosed as type 1 ROP but only 28 were identified by the WINROP algorithm.The sensitivity was 56%(28/50)and the NPV was 92%(252/274).However,for infants with BW<1000g or GA<28 wk,the sensitivity was 93.8%(15/16)and 93.3%(14/15),respectively.Meanwhile,with several postnatal complications added as additional risk factors,the sensitivity was increased to 96%(48/50).CONCLUSION:The sensitivity of the WINROP algorithm from the Southern Chinese cohort is not as high as that reported in developed countries.This algorithm is effective for detecting severe ROP from extremely small or preterm infants.Modification of the algorithm with additional risk factors could improve the predictive value for infants with a GA>28 wk in China.

Expression of Angiotensin Ⅱ and Aldosterone in Radiation-induced Lung Injury

Objective Radiation-induced lung injury(RILI) is the most common,dose-limiting complication in thoracic malignancy radiotherapy.Considering its negative impact on patients and restrictions to efficacy,the mechanism of RILI was studied. Methods Wistar rats were locally irradiated with a single dose of 0,16,and 20 Gy to the right half of the lung to establish a lung injury model.Two and six months after irradiation,the right half of the rat lung tissue was removed,and the concentrations of TGF-β1,angiotensinⅡ,and aldosterone were determined via enzyme-linked immunosorbent assay. Results Statistical differences were observed in the expression levels of angiotensinⅡand aldosterone between the non-irradiation and irradiation groups.Moreover,the expression level of the angiotensinⅡ-aldosterone system increased with increasing doses,and the difference was still observed as time progressed. Conclusions AngiotensinⅡ-aldosterone system has an important pathophysiological function in the progression of RILI.

Climatic Characteristics of Hail and Risk Zoning of Its Disaster Factor in Anhui Province

[Objective]The research aimed to study climatic characteristics of hail and risk zoning of its disaster factor in Anhui Province.[Method]Based on the hail-day records from 82 weather stations in Anhui Province,Chinese Meteorological Disaster Dictionary(1951 to 2005)and The Yearbook of Meteorological Disaster in Anhui Province(2006 to 2009),we discussed the climatic characteristic and risk zoning of hail disaster in Anhui Province.[Result]The hail days exhibited significant interdecadal variability,and it had a significant negative correlation with annual mean minimum temperature.The hail usually occurred from March to August,most frequently in June but less in autumn and winter.It most likely took place from afternoon to sunset.The spatial distribution showed that the hail occurred more in northeast of Huaibei,south of Dabie Mountain and some areas of Huangshan Mountain,but less in Jianghuai region.The index of hail intensity was defined by three factors,the diameter,duration and gust grade,and the percentile method was used to grade the intensity of the hail disaster.This work provided a possibility for quantitative assessment of the intensity of hail disaster.The results of hail disaster risk zoning in Anhui Province showed that high risk zones were concentrated in northeast of Huaibei,and low risk zones were mainly in south of Huaihe River.Finally,verified by historic disaster,it showed that the results of risk zoning had a certain rationality and feasibility.[Conclusion]The research could provide reference basis for hail disaster defense and assessment.

城市燃气企业发展氢能产业关键技术分析

氢气是燃气的一种,是未来绿色燃气的重要组成部分,氢能与天然气的深度融合可促进燃气行业高质量发展。本文介绍了国内外燃气企业在氢能及燃料电池领域的探索,分析了氢能及燃料电池在城镇燃气中应用的关键技术。面向未来,充分发挥燃气企业在危化品管理和管网基础设施等方面的优势,促进天然气与氢能的深入融合,加速推进“氢进万家”,助力推动燃气行业的碳中和目标的实现。

Observation of Zeeman splitting effect in a laser-driven coil

The Zeeman splitting effect is observed in a strong magnetic field generated by a laser-driven coil.The expanding plasma from the coil wire surface is concentrated at the coil center and interacts with the simultaneously generated magnetic field.The Cu I spectral lines at wavelengths of 510.5541,515.3235,and 521.8202 nm are detected and analyzed.The splittings of spectral lines are used to estimate the magnetic field strength at the coil center as∼31.4±15.7 T at a laser intensity of∼5.6310^(15) W/cm^(2),which agrees well with measurements using a B-dot probe.Some other plasma parameters of the central plasma disk are also studied.The temperature is evaluated from the Cu I spectral line intensity ratio,while the electron density is estimated from the Stark broadening effect.

Effects of pestle needle on nerve conduction velocity and inflammatory injury in patients with diabetic peripheral neuropathy

Background:Diabetic peripheral neuropathy(DPN)is one of the most common complications of diabetes mellitus.Impaired neurological function is one of the main characteristics of DPN and is strongly associated with the inflammatory response.Our previous studies have confirmed that pestle needle can improve the nerve function of patients with DPN.But the mechanism of pestle needle treatment of DPN is still unclear.Methods:A total of 70 DPN patients who met the inclusion criteria were randomly divided into two groups.Control group(CG)(n=35)received DPN conventional treatment and the pestle needle group(PNG)(n=35)received pestle needle therapy at Zhiyang(DU09)eight array,Mingmen(DU04)eight array and Heche Road(from Mingmen(DU04)to Changqiang(DU01)),Zusanli(ST36),Sanyinjiao(SP06),Taixi(KI03)and Yongquan(KI01).Patients in the PNG group were required to take this treatment for 4 weeks,5 times a week.Examination indexes were collected before and after treatment,respectively.Nerve function was examined using the Toronto clinical scoring system and nerve conduction velocity detection.Serum inflammatory factors were measured by enzyme linked immunosorbent assay.Results:The Toronto clinical scoring system was significantly reduced in the PNG compared with the CG after treatment.The sensory nerve conduction velocity and motor nerve conduction velocity of the right peroneal and median nerves were significantly faster in the PNG than those in the CG(P<0.05).After treatment,serum interleukin-1 beta,interleukin-6 and tumor necrosis factor-alpha levels decreased in both groups,and the improvement of PNG was better than CG(P<0.05).Conclusion:The pestle needle can significantly improve the symptoms and nerve conduction velocity of DPN,and its mechanism may be related to the reduction of inflammatory factors.

Synthesis and Cytotoxicities of Royleanone Derivatives

Carnosic acid was used as starting material to synthesize royleanone derivatives featured C11–C14 para quinone.The importance of C-20 group of royleanone derivatives was verified by the cytotoxicity assay of royleanonic acid,miltionone I and deoxyneocrptotanshinone.Following our synthetic route,15 amide derivatives were synthesized and 8 compounds exhibited moderate cytotoxic activities against three human cancer lines in vitro.

The Effect of Survivin siRNA on Apoptosis, Proliferation and Invasion by a Colon Carcinoma Cell Line

OBJECTIVE To study the efficiency of silencing survivin gene expression by an anti-survivin small RNA expression plasmid and to examine its impact on apoptosis, proliferation and invasive ability of the SW480 colorec-tal cancer cell line. METHODS An expression plasmid of survivin siRNA (pRNAT/sur-siR-NA) was constructed and transduced into SW480 cells. Western blot analysis and a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) were used to measure the change in expression level of the survivin protein and mRNA after transduction of the expression plasmid into the SW480. Apoptosis, proliferation, and invasive ability of the SW480 cells was evaluated by flow cytometry, MTT assay and Boy-den Chamber Assay, respectively. RESULTS The expression plasmid (pRNAT/sur-siRNA) against survivin down-regulated survivin protein and mRNA expression dramatically, with a down-regulation of 85% and 80%, respectively. After transfection of the pRNAT/sur-siRNA to the SW480 cells, the apoptotic rate of the pRNAT/ sur-siRNA/SW480 cells was 16.9%, and the proliferation rate was 37.4%, significantly different compared to the controls (P<0.01). Cell invasive studies (Boyden Chamber Assay) showed that number of cells penetrating the membrane for the pRNAT/sur-siRNA/SW480, pRNAT/SW480 and the SW480 cells was 153±66, 505±65 and 578±98, respectively (P<0.01). CONCLUSION siRNA against survivin can significantly inhibit the expression of survivin in SW480 cells, and thus promote apoptosis, inhibit proliferation and invasive ability.