Non-coding RNAs(ncRNAs),that is,RNAs not translated into proteins,are crucial regulators of a variety of biological processes in plants.While protein-encoding genes have been relatively well-annotated in sequenced gen...Non-coding RNAs(ncRNAs),that is,RNAs not translated into proteins,are crucial regulators of a variety of biological processes in plants.While protein-encoding genes have been relatively well-annotated in sequenced genomes,accounting for a small portion of the genome space in plants,the universe of plant ncRNAs is rapidly expanding.Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs.Here we summarize the classification and known biological functions of plant ncRNAs,review the application of next-generation sequencing(NGS)technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies,especially the new genome-editing tool clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated protein 9(Cas9)systems,to functional characterization of plant ncRNAs.展开更多
Peanut (Arachis hypogaea L.) is one of the major oilseed crops, mainly grown in tropical and sub-tropical regions of the world. It is also rich in proteins, vitamins and ions, therefore it constitutes an important por...Peanut (Arachis hypogaea L.) is one of the major oilseed crops, mainly grown in tropical and sub-tropical regions of the world. It is also rich in proteins, vitamins and ions, therefore it constitutes an important portion of food nutrition for people in these regions. The production of peanut is being threatened by the changing environments as the major peanut producing counties such as China, India, and USA are facing severe water shortage for peanut irrigation. The yield and quality of peanut are negatively affected by drought and salinity. Making peanut more droughtand salt-tolerant will likely sustain peanut production in countries where water shortage or saline soil are already problems. Efforts were made to genetically engineer peanut for higher tolerance to drought and salt. Analysis of these transgenic peanut plants indicated that the agronomic traits such as peanut yields were the same between wild-type and transgenic peanut plants under normal growth conditions, yet the yields of transgenic peanut plants were much higher than wild-type peanut plant under reduced irrigation conditions. Other traits such as protein content and fatty acid compositions in the seeds of transgenic peanut plants were not altered under both normal and drought conditions, indicating that the genetic manipulation of peanut for stress tolerance did not affect chemical compositions of peanut seeds in transgenic peanut plants, only increased seed yields under stress conditions.展开更多
基金supported by the National Natural Science Foundation of China (20636030, 2090618, 21236001)the Natural Science Foundation of Hebei Province (B2017202226)~~
基金This research is supported by the Department of Energy(DOE),Office of Science,Genomic Science Program under Award Number DE-SC0008834Oak Ridge National Laboratory is managed by UT-Battelle,LLC for the US DOE under Contract Number DE-AC05-00OR22725.This manuscript has been authored by UT-Battelle,LLC under Contract No.DE-AC05-00OR22725 with the U.S.
文摘Non-coding RNAs(ncRNAs),that is,RNAs not translated into proteins,are crucial regulators of a variety of biological processes in plants.While protein-encoding genes have been relatively well-annotated in sequenced genomes,accounting for a small portion of the genome space in plants,the universe of plant ncRNAs is rapidly expanding.Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs.Here we summarize the classification and known biological functions of plant ncRNAs,review the application of next-generation sequencing(NGS)technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies,especially the new genome-editing tool clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated protein 9(Cas9)systems,to functional characterization of plant ncRNAs.
文摘Peanut (Arachis hypogaea L.) is one of the major oilseed crops, mainly grown in tropical and sub-tropical regions of the world. It is also rich in proteins, vitamins and ions, therefore it constitutes an important portion of food nutrition for people in these regions. The production of peanut is being threatened by the changing environments as the major peanut producing counties such as China, India, and USA are facing severe water shortage for peanut irrigation. The yield and quality of peanut are negatively affected by drought and salinity. Making peanut more droughtand salt-tolerant will likely sustain peanut production in countries where water shortage or saline soil are already problems. Efforts were made to genetically engineer peanut for higher tolerance to drought and salt. Analysis of these transgenic peanut plants indicated that the agronomic traits such as peanut yields were the same between wild-type and transgenic peanut plants under normal growth conditions, yet the yields of transgenic peanut plants were much higher than wild-type peanut plant under reduced irrigation conditions. Other traits such as protein content and fatty acid compositions in the seeds of transgenic peanut plants were not altered under both normal and drought conditions, indicating that the genetic manipulation of peanut for stress tolerance did not affect chemical compositions of peanut seeds in transgenic peanut plants, only increased seed yields under stress conditions.
