The necessity of pretreatment with 0.1%pranoprofen for femtosecond-assisted cataract surgery:A single-center,randomized controlled trial

Purpose:To explore the effect of the variation of pupil diameter(PD)and intraocular pressure(IOP)induced by femtosecond laser treatment on the subsequent phacoemulsfication and intraocular lens implantation.And whether the application of 0.1%pranoprofen could significantly reduce the miosis and increased IOP caused by femtosecond laser treatment in femtosecond laser-assisted cataract surgery(FLACS).Methods:In this study,patients were pretreated with(trial group)or without(control group)topical 0.1%pranoprofen.The PD and IOP were measured at different time points within 30 min after the completion of the femtosecond laser treatment.Results:The comparisons of the two groups showed the PD of patients pretreated with 0.1%pranoprofen was significantly larger than that of the control only at 15 min after FLACS(P=0.046),and there was no significant difference in IOP at any time point(P>0.05).Neither the ratio of significant miosis(PD≤5 mm)nor intraocular hypertension(IOP≥30 mmHg)was significantly different between the control group(1.72%,6.67%)and the trial group(1%,4.17%)(P>0.05).Conclusions:The PD and IOP of patients undergoing FLACS showed fluctuations within a small range.The rates of significant miosis and intraocular hypertension are very low,it is safe for surgeons to complete the follow-up procedures within 30 min after femtosecond laser treatment.Pretreatment with 0.1%pranoprofen exerted a slight,albeit significant prophylactic effect preventing pupil miosis.However,it provided only a limited benefit in patients undergoing FLACS without other complications.

Paired Immunoglobulin-like Receptor B Inhibition in Müller Cells Promotes Neurite Regeneration After Retinal Ganglion Cell Injury in vitro

In the central nervous system(CNS),three types of myelin-associated inhibitors(MAIs) have major inhibitory effects on nerve regeneration.They include Nogo-A,myelin-associated glycoprotein,and oligodendrocyte-myelin glycoprotein.MAIs possess two co-receptors,Nogo receptor(NgR) and paired immunoglobulin-like receptor B(PirB).Previous studies have confirmed that the inhibition of NgR only results in a modest increase in regeneration in the CNS;however,the inhibitory effects of PirB with regard to nerve regeneration after binding to MAIs remain controversial.In this study,we demonstrated that PirB is expressed in primary cultures of retinal ganglion cells(RGCs),and the inhibitory effects of the three MAIs on the growth of RGC neurites are not significantly decreased after direct PirB knockdown using adenovirus PirB shRNA.Interestingly,we found that retinal Müller cells expressed PirB and that its knockdown enhanced the regeneration of co-cultured RGC neurites.PirB knockdown also activated the JAK/Stat3 signaling pathway in Müller cells and upregulated ciliary neurotrophic factor levels.These findings indicate that PirB plays a novel role in retinal Müller cells and that its action in these cells may indirectly affect the growth of RGC neurites.The results also reveal that PirB in Müller cells affects RGC neurite regeneration.Our findings provide a novel basis for the use of PirB as a target molecule to promote nerve regeneration.