宣肺败毒颗粒缓解低温高湿环境下冠状病毒引起的肺炎

本研究旨在探讨宣肺败毒颗粒(XFBD)在寒冷和潮湿条件下的冠状病毒肺炎模型中的作用及其机制。为模拟冠状病毒感染小鼠模型,采用寒冷和潮湿条件下感染冠状病毒229E株。随后,XFBD在第五天开始给药,持续三天。通过评价行为特征、肺指数、病毒载量和肺部病理变化来评估XFBD对模型小鼠的治疗效果。通过酶联免疫吸附试验测定白细胞介素-6、白细胞介素-10和肿瘤坏死因子-α的水平。使用流式细胞术检测CD4+T细胞、CD8+T细胞和B细胞的比例。结果显示,对照组的小鼠活泼好动,状态良好,毛发光亮。造模后,模型小鼠表现出活动减少、毛发杂乱且无光泽、食欲不振。服用不同剂量的XFBD后,冠状病毒肺炎小鼠的症状均有作用改善。不同剂量的XFBD能显著下调炎症因子,调节CD4+T细胞、CD8+T细胞和B细胞的表达,并且高剂量XFBD的疗效更佳。综上所述,XFBD对寒冷和潮湿条件下感染的冠状病毒肺炎小鼠有良好的治疗作用,其能改善小鼠的行为特征和胃肠道指数,降低肺部病毒滴度和组织病理学改变,这与抑制炎症反应和增加淋巴细胞数量有关。

Panoramic comparison between NK cells in healthy and cancerous liver through single-cell RNA sequencing

Objective:NK cells play crucial roles in the immune defense mechanisms against viral infections and transformed cells.However,the developmental progression,transcriptomic landscape,and functional subtypes of liver NK cells are not well defined.Hepatocellular carcinoma(HCC)accounts for approximately 80%of primary liver cancer worldwide,yet the biological characteristics of NK cells in the HCC environment are unclear.Therefore,we aimed to determine these cells’roles in tumorigenesis and prognosis.Methods:We compared the single-cell RNA sequencing profiles of NK cells purified from blood(n=1),healthy liver tissues(n=3),HCC tumor tissues(n=4),and peritumor liver tissues(n=1)to identify NK cell subsets.Furthermore,we performed bioinformatics analysis by using The Cancer Genome Atlas(TCGA)data to identify prognostic biomarkers simultaneously overexpressed in the blood and tumor tissues of patients with HCC.Results:Transcriptomic analysis revealed 5 NK cell subsets(L1-NK-CD56bright,L2-NK-CD56dim,L3-NK-HLA,L4-LrNK-FCGR3A,and L5-LrNK-XCL1)in the healthy liver tissues.However,the transitional L3 subset and the CXCR6+CD16+L4 subset with strong anti-tumor activity were absent in the HCC and peritumor liver tissues.Furthermore,4 common prognosis-associated genes(RHOB,TALDO1,HLA-DPA1,and TKT)were significantly overexpressed in the paired tumor tissue and blood.Conclusions:Our study revealed 5 specific subsets of NK cells in healthy human liver tissues.However,only 3 of the 5 NK cell subsets were present in HCC and peritumor tissues.The cytotoxic NK cell subsets were absent in HCC tissues.Furthermore,we identified 4 potential non-invasive prognostic biomarkers in patients with HCC.

Proteomics analysis of liver proteins from rats with spleen-deficiency syndrome induced by chronic improper diet consumption and fatigue

Objective:To investigate a proteomics analysis of liver proteins from rats with spleen-deficiency syndrome (SDS) induced by chronic improper diet consumption and fatigue.Methods:This study used a liver proteomic profiling method to identify differentially expressed proteins and altered pathways involved in SDS rats.Specifically,we collected liver samples from a control group and a group with SDS induced by chronic improper diet consumption and fatigue for 4 weeks.The pooled liver proteins in each group were labeled with 8-plex isobaric tags for relative and absolute quantitation reagents.The labeled control and SDS group samples were pooled together and separated by high-pH reverse-phase liquid chromatography.The differentially expressed proteins from the liver proteomes were analyzed to identify potential biomarkers of SDS.Differentially expressed proteins were selected in conjunction with gene ontology and ingenuity pathway analysis.Results:We identified 2176 protein clusters with more than two peptides in the SDS group,with 141 proteins quantified as differentially expressed proteins.Of these,75 proteins were up-regulated,and 66 were down-regulated.Three activated signaling pathways,the thrombin,CXCR4 and synaptic long term depression signaling pathways,and a large multi-protein complex within the network were revealed in the liver proteomic analysis of SDS rats.Conclusions:This is the first report of a differential liver proteome under SDS conditions.The results suggest that the liver proteome partially reflects the pathological changes involved in SDS.The findings provide important information for comprehensively understanding the mechanisms of dysfunction or injury in the liver at the molecular level as a result of SDS.Furthermore,they provide a novel understanding of the connotation of SDS in the fleld of traditional Chinese Medicine.

Clinical analysis of acute cerebrovascular disease

Objective: To analyze the clinical characteristics of acute cerebrovascular disease. Methods: 263 patients with acute cerebrovascular disease admitted in our hospital from January 2013 to January 2015 were selected as the study objects and their clinical data were analyzed. Results: The incidence of acute cerebrovascular disease was higher in males than in females (1.8:1). The morbidity of acute cerebrovascular disease was higher over 60 years old. The incidence time of acute cerebrovascular disease was 6:00-10:00, and hypertension was the primary risk factor of this disease. Conclusions: The mortality rate of acute cerebrovascular disease was high, and there were many risk factors associated with the disease. The prevention and treatment of acute cerebrovascular disease should be strengthened.

大蜜蜂的生物学特性、面临威胁与保护策略

昆虫传粉在维持植物的有性繁殖、物种形成及生态系统稳定中扮演着重要角色,而野生传粉昆虫为生态系统提供了巨大的传粉服务功能。大蜜蜂(Apis dorsata)为亚洲特有的一种野生传粉昆虫,是热带地区多种植物和农作物的有效传粉者,在保障热带生物多样性及作物产量中有不可或缺的作用。但受全球气候变化、人类活动和生境恶化等因素的影响,其种群数量日益减少,开展大蜜蜂种质资源保护势在必行。本文综述了大蜜蜂筑巢、迁飞和传粉服务功能,分析了人为猎取蜂巢,栖息生境遭受破坏,杀虫剂和除草剂滥用,昆虫、螨类和病原物侵染,气候变化等威胁种群的因素,以期从强化大蜜蜂基础研究和保护、推动生态农业发展、建立适合大蜜蜂迁飞生态廊道、加强检验检疫及科学合理利用大蜜蜂种质资源等方面制定相应的保护措施。