Grass carp is one of the most important cultured fishes all over the world.The genetic diversity of grass carp plays an important role whatever in selective breeding progress or ecological conservation purposes.Howeve...Grass carp is one of the most important cultured fishes all over the world.The genetic diversity of grass carp plays an important role whatever in selective breeding progress or ecological conservation purposes.However,some genetic diversity researches were not accuracy and cannot be compared with each other due to different molecular markers,sample size and detection methods.In this study,we constructed five multiplex PCR assays contained 20 microsatellites with highly polymorphism and heterozygosity for evaluating genetic information of grass carp.We used nine cultured populations consisting of 507 individuals to detect stability of the five multiplex PCR assays.The results showed that the number of alleles(Na),effective number of alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He)and polymorphism information content(PIC)of the 20 microsatellite loci were relative high compared with the genetic diversity parameters of microsatellite loci developed by other researchers.Six loci were significantly deviated from Hardy-Weinberg equilibrium(P<0.01).And with exception of the Shaoguan,Indian and Nepal cultured population,all other cultured populations showed very high genetic diversity.Through the test of grass carp populations,we developed an effective and accurate multiplex SSR-PCR assays that can be as statistical powerful tool for detecting genetic information of grass carp.展开更多
The Chinese lake gudgeon is a small benthopelagic freshwater fish.It is presently threatened by human activities and environmental factors in China.Understanding the genetic diversity and population structure is funda...The Chinese lake gudgeon is a small benthopelagic freshwater fish.It is presently threatened by human activities and environmental factors in China.Understanding the genetic diversity and population structure is fundamental for implementation of appropriate conservation measures and a sustainable management program.However,little is reported about the current genetic diversity and population structure.Here,we used ten microsatellite markers to genotype 175 individuals from six populations.Low levels of genetic diversity were found in all six tested populations.The Xiang river population showed the highest level of genetic diversity.Genetic differentiation was very low but significant among the Changyang,Changqidang and Mayang populations,but the Qiandao lake,Gan river,Xiang river populations all showed significant and strong differentiation from the other three populations.Contemporary gene flow was observed in among Changyang,Changqidang and Mayang populations and between Gan and Xiang river populations,respectively.This is the first genetic study to report the genetic diversity and population structure of S.sinensis and the results will be used to develop management and conservation strategies.展开更多
基金This work was supported by grants from the China's Agricultural Research System(CARS-46-04)the Project of Shanghai Engineering and Technology Center for Promoting Ability(16DZ2281200).
文摘Grass carp is one of the most important cultured fishes all over the world.The genetic diversity of grass carp plays an important role whatever in selective breeding progress or ecological conservation purposes.However,some genetic diversity researches were not accuracy and cannot be compared with each other due to different molecular markers,sample size and detection methods.In this study,we constructed five multiplex PCR assays contained 20 microsatellites with highly polymorphism and heterozygosity for evaluating genetic information of grass carp.We used nine cultured populations consisting of 507 individuals to detect stability of the five multiplex PCR assays.The results showed that the number of alleles(Na),effective number of alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He)and polymorphism information content(PIC)of the 20 microsatellite loci were relative high compared with the genetic diversity parameters of microsatellite loci developed by other researchers.Six loci were significantly deviated from Hardy-Weinberg equilibrium(P<0.01).And with exception of the Shaoguan,Indian and Nepal cultured population,all other cultured populations showed very high genetic diversity.Through the test of grass carp populations,we developed an effective and accurate multiplex SSR-PCR assays that can be as statistical powerful tool for detecting genetic information of grass carp.
基金supported by the Jiangsu Key Technology R&D Program(BE2011330)。
文摘The Chinese lake gudgeon is a small benthopelagic freshwater fish.It is presently threatened by human activities and environmental factors in China.Understanding the genetic diversity and population structure is fundamental for implementation of appropriate conservation measures and a sustainable management program.However,little is reported about the current genetic diversity and population structure.Here,we used ten microsatellite markers to genotype 175 individuals from six populations.Low levels of genetic diversity were found in all six tested populations.The Xiang river population showed the highest level of genetic diversity.Genetic differentiation was very low but significant among the Changyang,Changqidang and Mayang populations,but the Qiandao lake,Gan river,Xiang river populations all showed significant and strong differentiation from the other three populations.Contemporary gene flow was observed in among Changyang,Changqidang and Mayang populations and between Gan and Xiang river populations,respectively.This is the first genetic study to report the genetic diversity and population structure of S.sinensis and the results will be used to develop management and conservation strategies.
