期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Expression of a fusion protein of human ciliary neurotrophic factor and soluble CNTF-Receptor and identification of its activity
1
作者 陈益 MārzPia +2 位作者 OttenUwe 葛霁光 rose-johnstefan 《Journal of Zhejiang University Science》 EI CSCD 2003年第3期340-345,共6页
Ciliary neurotrophic factor (CNTF) has pleiotropic actions on many neuronal populations as well as on glia. Signal transduction by CNTF requires that it bind first to CNTF R, permitting the recruitment of gp130 and L... Ciliary neurotrophic factor (CNTF) has pleiotropic actions on many neuronal populations as well as on glia. Signal transduction by CNTF requires that it bind first to CNTF R, permitting the recruitment of gp130 and LIF R, forming a tripartite receptor complex. Cells that only express gp130 and LIF R, but not CNTF R are refractory to stimulation by CNTF. On many target cells CNTF only acts in the presence of its specific agonistic soluble receptors. We engineered a soluble fusion protein by linking the COOH terminus of sCNTF R to the NH 2 terminus of CNTF. Recombinant CNTF/sCNTF R fusion protein (Hyper CNTF) was successfully expressed in COS 7 cells. The apparent molecular mass of the Hyper CNTF protein was estimated from western blots to be 75 kDa. Proliferation assays of transfected BAF/3 cells in response to CNTF and Hyper CNTF were used to verify the activity of the cytokines. The proliferative results confirmed that CNTF required homodimerization of the gp130, CNTF R and LIF R receptor subunit whereas Hyper CNTF required heterodimerization of the gp130 and LIF R receptor subunit. We concluded that the fusion protein Hyper CNTF had superagonistic activity on target cells expressing gp130 and LIF R, but lacking membrane bound CNTF R. 展开更多
关键词 Ciliary neurotrophic factor(CNTF) Soluble CNTF Receptor Fusion protein Biological activity
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部