The illicit addition of phosphodiesterase type-5 （PDE-5） inhibitors like sildenafil （Viagra） in product offered as herbal medicine or dietary supplement for male erectile dysfunction has concerned authorities in r...The illicit addition of phosphodiesterase type-5 （PDE-5） inhibitors like sildenafil （Viagra） in product offered as herbal medicine or dietary supplement for male erectile dysfunction has concerned authorities in recent times. In this paper, we proposed a sensitive surfactant-coated Amberlite XADTM resin for sildenafil preconcentration method with spectrofluorimetric detection. Retention capacity of micellar coated XAD resin for sildenafil was studied and the obtained eluate was measured by spectrofluorometer at excitation and emission wavelengths of 350 and 430 nm, respectively. This method allowed the detection of sildenafil at 0.15 ng/mL with linear range of 0.0003-7.0 μg/mL. The method has been successfully applied to the analysis of some local commercially available herbal medicines and urine.展开更多
基金INQUISAL-CONICET (Instituto de Química de San Luis-Consejo Nacional de Investigaciones Científicas y Tecnológicas)FONCYT (Fondo Nacional de Ciencia y Tecnología)the National University of San Luis (Projects SECyT-UNSL 22/Q828 and PIP-CONICET 11220100100405) for their financial support
文摘The illicit addition of phosphodiesterase type-5 （PDE-5） inhibitors like sildenafil （Viagra） in product offered as herbal medicine or dietary supplement for male erectile dysfunction has concerned authorities in recent times. In this paper, we proposed a sensitive surfactant-coated Amberlite XADTM resin for sildenafil preconcentration method with spectrofluorimetric detection. Retention capacity of micellar coated XAD resin for sildenafil was studied and the obtained eluate was measured by spectrofluorometer at excitation and emission wavelengths of 350 and 430 nm, respectively. This method allowed the detection of sildenafil at 0.15 ng/mL with linear range of 0.0003-7.0 μg/mL. The method has been successfully applied to the analysis of some local commercially available herbal medicines and urine.
