MiR-194-5p suppresses the warburg effect in ovarian cancer cells through the IGF1R/PI3K/AKT axis

Background:The Warburg effect is considered as a hallmark of various types of cancers,while the regulatory mechanism is poorly understood.Our previous study demonstrated that miR-194-5p directly targets and regulates insulin-like growth factor1 receptor(IGF1R).In this study,we aimed to investigate the role of miR-194-5p in the regulation of the Warburg effect in ovarian cancer cells.Methods:The stable ovarian cell lines with miR-194-5p overexpression or silencing IGF1R expression were established by lentivirus infection.ATP generation,glucose uptake,lactate production and extracellular acidification rate(ECAR)assay were used to analyze the effects of aerobic glycolysis in ovarian cancer cells.Gene expression was analyzed by quantitative polymerase chain reaction(qPCR)and western blot.Immunohistochemistry assays were performed to assess the expression of the IGF1R protein in ovarian cancer tissues.Results:Overexpression of miR-194-5p or silencing IGF1R expression in ovarian cancer cells decreases ATP generation,glucose uptake,lactate production,and ECAR and inhibits both the mRNA and protein expression of PKM2,LDHA,GLUT1,and GLUT3.While the knockdown of miR-194-5p expression led to opposite results.Overexpression of miR-194-5p or silencing IGF1R expression suppressed the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)pathway,whose activation can sustain aerobic glycolysis in cancer cells,and the knockdown of miR-194-5p expression promoted the activation of the PI3K/AKT pathway.Conclusion:Our results suggest that miR-194-5p can inhibit the Warburg effect by negative regulation of IGF1R and further repression of the PI3K/AKT pathway,which provides a theoretical basis for further test of miR-194-5p as a target in the treatment of ovarian cancer.

Study on Plugging Technology in Oil Test

In view of the shortcomings of poor temperature resistance, poor pumpability and poor pressure-bearing capacity of commonly used gel plugging materials, polyacrylamide (HPAM) and N,N-methylene bisacrylamide (BIS) were selected for crosslinking and compounding to make a new type of gel plugging material with high temperature and pressure resistance. The compressive strength and yield stress were measured by inverted observation method to evaluate the gel strength. The anti-temperature, anti-pressure, anti-dilution and gel-breaking properties of the gel were evaluated. Finally, 71 type high temperature and high pressure water loss instrument and high temperature and high pressure filter with slit plate were used to evaluate the plugging capacity of gel plugging agent. The experimental results show that the new gel plugging system between 100°C - 120°C, gelation time can be controlled at about 5 h;it has strong temperature resistance, compression resistance, dilution resistance and gel breaking performance. In the face of permeability and fracture leakage simulation experiments, when the ambient temperature below 120°C, pressure within 5 MPa, the filter loss of gel plugging agent is far less than the market two conventional plugging agent, has excellent plugging performance.

Surveying ionizing radiations in real time using a smartphone

Surveying ionizing radiations of the surrounding with a smartphone provides a low-cost and convenient utility for the general public. We developed a smartphone application(App) that uses the built-in camera with a CMOS sensor and a radiation signal extraction algorithm.After a calibration through a series of radiation exposures,the App could display radiation dose rate and cumulative dose in real time without requiring covering the camera lens. A smartphone with this App can be used as a fast survey tool for ionizing radiations.

Model of output characteristics of giant magnetoresistance(GMR) multilayer sensor

In this paper,the giant magnetoresistance(GMR)multilayer sensor is fabricated with a Wheatstone bridge,and it exhibits excellent performance with a sensitivity of 2.8349 mV/(V/Oe)(1 Oe=79.5775 A·m^-1)and a saturation field of 26 Oe along the sensitive axis.The GMR sensor is also characterized in a high magnetic field.The sensitivity decreases from 2.8349 mV/(V/Oe)at an angle of 0°to 0.0175 mV/(V/Oe)at an angle of 90°.Then,the sensor is placed in a series of rotating magnetic fields.We propose a model to express the output characteristics of the GMR multilayer sensor.The transfer curves of the sensor can be shown as two exactly symmetrical circles with an increasing radius when the magnetic field increases.The experimental results are consistent with the simulation results of the model.The advantage of this model is that it is simpler and more intuitive.

Advancing intestinal organoid technology to decipher nano-intestine interactions and treat intestinal disease

With research burgeoning in nanoscience and nanotechnology,there is an urgent need to develop new biological models that can simulate native structure,function,and genetic properties of tissues to evaluate the adverse or beneficial effects of nanomaterials on a host.Among the current biological models,three-dimensional(3D)organoids have developed as powerful tools in the study of nanomaterial-biology(nano-bio)interactions,since these models can overcome many of the limitations of cell and animal models.A deep understanding of organoid techniques will facilitate the development of more efficient nanomedicines and further the fields of tissue engineering and personalized medicine.Herein,we summarize the recent progress in intestinal organoids culture systems with a focus on our understanding of the nature and influencing factors of intestinal organoid growth.We also discuss biomimetic extracellular matrices(ECMs)coupled with nanotechnology.In particular,we analyze the application prospects for intestinal organoids in investigating nano-intestine interactions.By integrating nanotechnology and organoid technology,this recently developed model will fill the gaps left due to the deficiencies of traditional cell and animal models,thus accelerating both our understanding of intestine-related nanotoxicity and the development of nanomedicines.

STING and TLR9 agonists synergistically enhance the immunogenicity of SARS-CoV-2 subunit vaccine

Vaccines that are reliable and efficacious are essential in the fight against the COVID-19 pandemic.In this study,we designed a dual-adjuvant system with two pathogen-associated molecular patterns(PAMPs),MnOx and CpG.This system can improve the retention of antigens at the injection site,facilitate pro-inflammatory cytokines secretion,further recruit and activate dendritic cells(DCs).As a result,antigens can be delivered to lymph nodes specifically,and adaptive immunity was strengthened.The immunized group showed an enhanced and broadened humoral and cellular immune response in systemic immunity and lung protection when combined with a tandem repeat-linked dimeric antigen version of the SARS-CoV-2 receptor binding domain(RBDdimer).Remarkably,even with a significant reduction in antigen dosage(three times lower)and a decrease in injection frequencies,our nanovaccine was able to produce the highest neutralizing antibody titers against various mutants.These titers were four-fold higher for the wild-type strain and two-fold higher for both the Beta and Omicron variants in comparison with those elicited by the Alum adjuvant group.In conclusion,our dual-adjuvant formulation presents a promising protein subunit-based candidate vaccine against SARS-CoV-2.

西部高校人才管理的双重困境及其出路——基于“推力—拉力”理论的系统阐释

知识经济时代,人才资源作为社会经济发展中第一资源的地位更加突出、更加明显,人才竞争已经成为一个国家或地区综合实力竞争的核心内容。西部高校在西部地区人才管理中具有核心地位,加强人才管理是西部高校以及西部地区实现跨越式发展面临的紧迫任务。人才管理的核心是人才引进和人才保留。本研究借鉴“推力—拉力”理论框架,分析了西部高校人才管理中引不来和留不住的双重困境,并从“推力”“拉力”和“阻力”三个方面,阐述了西部高校人才管理双重困境的原因,文章指出:“刚”(行政干预)“柔”(市场机制)并济促“四留”,即“待遇留人”“事业留人”“感情留人”和“制度留人”的协同治理,是西部高校走出人才管理困境的根本出路。

Field-free switching through bulk spin−orbit torque in L10-FePt films deposited on vicinal substrates

L1_(0)-FePt distinguishes itself for its ultrahigh perpendicular magnetic anisotropy(PMA),enabling thermally stabile memory cells to scale down to 3 nm.The recently discovered“bulk”spin−orbit torques in L1_(0)-FePt provide an efficient and scalable way to manipulate the L1_(0)-FePt magnetization.However,the existence of an external field during the switching limits its practical application,and therefore field-free switching of L1_(0)-FePt is highly demanded.In this manuscript,by growing the L1_(0)-FePt film on vicinal MgO(001)substrates,we realize the field-free switching of L1_(0)-FePt.This method is different from previously established strategies as it does not need to add other functional layers or create asymmetry in the film structure.The dependence on the vicinal angle,film thickness,and growth temperature demonstrates a wide operation window for the fieldfree switching of L1_(0)-FePt.We confirm the physical origin of the field-free switching is due to the tilted anisotropy of L1_(0)-FePt induced by the vicinal surface.We also quantitatively characterize the spin-orbit torques in the L1_(0)-FePt films.Our results extend beyond the established strategies to realize field-free switching,and potentially could be applied to mass production.