BACKGROUND Surgical site infection(SSI)is a common complication of colorectal surgery.Minimally invasive surgery notably reduces the incidence of SSI.This study aimed to compare the incidences of SSI after robot-assis...BACKGROUND Surgical site infection(SSI)is a common complication of colorectal surgery.Minimally invasive surgery notably reduces the incidence of SSI.This study aimed to compare the incidences of SSI after robot-assisted colorectal surgery(RACS)vs that after laparoscopic assisted colorectal surgery(LACS)and to analyze associated risk factors for SSI in minimally invasive colorectal surgery.AIM To compare the incidences of SSI after RACS and LACS,and to analyze the risk factors associated with SSI after minimally invasive colorectal surgery.METHODS Clinical data derived from patients who underwent minimally invasive colorectal surgery between October 2020 and October 2022 at the First Affiliated Hospital of Soochow University were collated.Differences in clinical characteristics and surgeryrelated information associated with RACS and LACS were compared,and possible risk factors for SSI were identified.RESULTS A total of 246 patients(112 LACS and 134 RACS)were included in the study.Fortythree(17.5%)developed SSI.The proportions of patients who developed SSI were similar in the two groups(17.9%vs 17.2%,P=0.887).Diabetes mellitus,intraoperative blood loss≥100 mL,and incision length were independent risk factors for SSI.Possible additional risk factors included neoadjuvant therapy,lesion site,and operation time.CONCLUSION There was no difference in SSI incidence in the RACS and LACS groups.Diabetes mellitus,intraoperative blood loss≥100 mL,and incision length were independent risk factors for postoperative SSI.展开更多
Objective:Androgen deprivation therapy(ADT)is still the principal treatment option for prostate cancer(PCa).In addition to reactivation of androgen receptor signaling,the resistance of PCa to apoptosis during ADT also...Objective:Androgen deprivation therapy(ADT)is still the principal treatment option for prostate cancer(PCa).In addition to reactivation of androgen receptor signaling,the resistance of PCa to apoptosis during ADT also contributes to castration resistant PCa(CRPC).A previous study reported that gene transfer of IL-13Rα2 into PCa cells sensitized the cells to the IL-13R-targeted cytotoxin IL13Rα1,leading to apoptosis.Compared with IL-13Rα2,IL13Rα1 is more constitutively expressed in PCa cells,but its function in PCa remains to be established.Methods:We determined the role and expression of IL13Rα1 in PCa cancer cells using western blotting,flow cytometry,and cell proliferation assays.Co-immunoprecipitation and mass spectrometry were used to identify the proteins that interacted with IL13Rα1,to elucidate its function.Results:In this study,we showed that IL13Rα1 was selectively suppressed in androgen-deprived PCa cells and that its suppression tended to be associated with poor prognoses of PCa patients.IL13Rα1 overexpression promoted apoptosis and inhibited tumor growth under androgen-deprived or castrated conditions(P<0.01).Mechanistically,IL13Rα1 recruited and facilitated ubiquitin protein ligase E3C-mediated ubiquitination and degradation of hexokinase 2(HK2),resulting in glycolytic inhibition and eventually leading to PCa cell apoptosis.Furthermore,our data revealed that mutated ataxia-telangiectasia kinase phosphorylated and facilitated the selective ubiquitin proteasome-mediated degradation of HK2.Notably,IL13Rα1-overexpressing PCa cells were more susceptible to apoptosis and exhibited reduced tumor growth after exposure to the HK2 inhibitor,2-deoxy-D-glucose(P<0.01).Conclusions:Our data identified a tumor suppressor role for IL13Rα1 in preventing the resistance of PCa cells to apoptosis during androgen deprivation by inhibiting glycolysis.IL13Rα1-mediated signaling involving HK2 may therefore provide a novel treatment target and strategy for CRPC.展开更多
Series of Fe/Cu-SSZ-13 catalysts with different Fe loading content were synthesized by simple one-pot strategy.The obtained catalysts were subjected to selective catalytic reduction(SCR)of NO x with NH 3 and were char...Series of Fe/Cu-SSZ-13 catalysts with different Fe loading content were synthesized by simple one-pot strategy.The obtained catalysts were subjected to selective catalytic reduction(SCR)of NO x with NH 3 and were characterized by various techniques.The results show that Fe 0.63/Cu 1.50-SSZ-13 catalyst with proper Fe content exhibits excellent catalytic activity with widest operation temperature window from 160 to 580℃,excellent hydrothermal stability as well as good resistance to sulfur poisoning when compared with Cu-SSZ-13,signifying its great potential for practical applications.Further characterizations reveal that the synthesized Fe/Cu-SSZ-13 catalysts present typical chabazite(CHA)structure with good crystallinity,while isolated Cu^2+and monomeric Fe 3+are revealed as the predominant copper and iron species.At low temperatures,isolated Cu^2+species act as primary active sites for SCR reaction,while monomeric Fe^3+species provide sufficient active sites for sustain the SCR activity at high temperature.Moreover,Fe over doping would lead to the damage of zeolite structure,destruction of isolated Cu^2+site,as well as the formation of highly oxidizing Fe2O3,thus causing deterioration of catalytic performances.展开更多
Objective:Parthenolide(PTL)induces anti-tumor effects via the nuclear factor kappa B(NF-κB)signaling pathway.MCL3,a PTL derivative,is a sesquiterpene lactone synthesized by the rearrangement and subsequent oxidation ...Objective:Parthenolide(PTL)induces anti-tumor effects via the nuclear factor kappa B(NF-κB)signaling pathway.MCL3,a PTL derivative,is a sesquiterpene lactone synthesized by the rearrangement and subsequent oxidation of PTL.The aim of this study was to elucidate the antitumor activity and mechanism of action of MCL3 in glioblastoma(GBM).Materials and Methods:The effects of MCL3 on G422 cell proliferation,apoptosis,invasion,and angiogenesis in vitro were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,flow cytometry,the cell invasion,and tube formation assays.The subcutaneously transplanted G422 xenograft model was used to detect the effect of MCL3 on tumor growth in vivo.Pathological changes were analyzed by immunohistochemical staining.The effects of MCL3 on NF-κB and Stat3 transcriptional activities were examined using a dual-luciferase reporter assay.Protein levels related to the NF-κB/interleukin(IL)-6/Stat3 signaling pathway were determined using western blot analysis.Results:MCL3 inhibited GBM cell proliferation,invasion,and angiogenesis in a concentration-dependent manner.Moreover,MCL3 decreased the transcriptional activities of NF-κB and Stat3.MCL3 suppressed tumor growth in the subcutaneously transplanted G422 xenograft model,while the inhibition rate was 79%in tumor weight at 40.0 mg/kg.MCL3 blocked the NF-κB/IL-6/Stat3 signaling pathway in G422 cells and tumor tissues,resulting in the downregulation of Stat3 target genes related to apoptosis,invasion,etc.,Conclusion:The results show that MCL3 might inhibit G422 GBM growth partly due to the inhibition of the NF-κB/IL-6/Stat3 signaling pathway.展开更多
文摘BACKGROUND Surgical site infection(SSI)is a common complication of colorectal surgery.Minimally invasive surgery notably reduces the incidence of SSI.This study aimed to compare the incidences of SSI after robot-assisted colorectal surgery(RACS)vs that after laparoscopic assisted colorectal surgery(LACS)and to analyze associated risk factors for SSI in minimally invasive colorectal surgery.AIM To compare the incidences of SSI after RACS and LACS,and to analyze the risk factors associated with SSI after minimally invasive colorectal surgery.METHODS Clinical data derived from patients who underwent minimally invasive colorectal surgery between October 2020 and October 2022 at the First Affiliated Hospital of Soochow University were collated.Differences in clinical characteristics and surgeryrelated information associated with RACS and LACS were compared,and possible risk factors for SSI were identified.RESULTS A total of 246 patients(112 LACS and 134 RACS)were included in the study.Fortythree(17.5%)developed SSI.The proportions of patients who developed SSI were similar in the two groups(17.9%vs 17.2%,P=0.887).Diabetes mellitus,intraoperative blood loss≥100 mL,and incision length were independent risk factors for SSI.Possible additional risk factors included neoadjuvant therapy,lesion site,and operation time.CONCLUSION There was no difference in SSI incidence in the RACS and LACS groups.Diabetes mellitus,intraoperative blood loss≥100 mL,and incision length were independent risk factors for postoperative SSI.
基金supported by the National Natural Science Foundation of China(Grant Nos.81772760 and 82072850)the Natural Science Foundation of Shandong Province(Grant Nos.ZR2020YQ55 and ZR2020QH327),the Shandong Taishan Scholarship(Grant No.tsqn20161076)+1 种基金the Innovation Project of Shandong Academy of Medical Sciences(2020)the program for Outstanding PhD candidate of Shandong University(2020)and Academic promotion programme of Shandong First Medical University(LJ001).
文摘Objective:Androgen deprivation therapy(ADT)is still the principal treatment option for prostate cancer(PCa).In addition to reactivation of androgen receptor signaling,the resistance of PCa to apoptosis during ADT also contributes to castration resistant PCa(CRPC).A previous study reported that gene transfer of IL-13Rα2 into PCa cells sensitized the cells to the IL-13R-targeted cytotoxin IL13Rα1,leading to apoptosis.Compared with IL-13Rα2,IL13Rα1 is more constitutively expressed in PCa cells,but its function in PCa remains to be established.Methods:We determined the role and expression of IL13Rα1 in PCa cancer cells using western blotting,flow cytometry,and cell proliferation assays.Co-immunoprecipitation and mass spectrometry were used to identify the proteins that interacted with IL13Rα1,to elucidate its function.Results:In this study,we showed that IL13Rα1 was selectively suppressed in androgen-deprived PCa cells and that its suppression tended to be associated with poor prognoses of PCa patients.IL13Rα1 overexpression promoted apoptosis and inhibited tumor growth under androgen-deprived or castrated conditions(P<0.01).Mechanistically,IL13Rα1 recruited and facilitated ubiquitin protein ligase E3C-mediated ubiquitination and degradation of hexokinase 2(HK2),resulting in glycolytic inhibition and eventually leading to PCa cell apoptosis.Furthermore,our data revealed that mutated ataxia-telangiectasia kinase phosphorylated and facilitated the selective ubiquitin proteasome-mediated degradation of HK2.Notably,IL13Rα1-overexpressing PCa cells were more susceptible to apoptosis and exhibited reduced tumor growth after exposure to the HK2 inhibitor,2-deoxy-D-glucose(P<0.01).Conclusions:Our data identified a tumor suppressor role for IL13Rα1 in preventing the resistance of PCa cells to apoptosis during androgen deprivation by inhibiting glycolysis.IL13Rα1-mediated signaling involving HK2 may therefore provide a novel treatment target and strategy for CRPC.
基金the Key Program of Science Technology Department of Zhejiang Province(No.2018C03037)the Scientific Research Fund of Nanjing Institute of Technology(No.YKJ2019111)。
文摘Series of Fe/Cu-SSZ-13 catalysts with different Fe loading content were synthesized by simple one-pot strategy.The obtained catalysts were subjected to selective catalytic reduction(SCR)of NO x with NH 3 and were characterized by various techniques.The results show that Fe 0.63/Cu 1.50-SSZ-13 catalyst with proper Fe content exhibits excellent catalytic activity with widest operation temperature window from 160 to 580℃,excellent hydrothermal stability as well as good resistance to sulfur poisoning when compared with Cu-SSZ-13,signifying its great potential for practical applications.Further characterizations reveal that the synthesized Fe/Cu-SSZ-13 catalysts present typical chabazite(CHA)structure with good crystallinity,while isolated Cu^2+and monomeric Fe 3+are revealed as the predominant copper and iron species.At low temperatures,isolated Cu^2+species act as primary active sites for SCR reaction,while monomeric Fe^3+species provide sufficient active sites for sustain the SCR activity at high temperature.Moreover,Fe over doping would lead to the damage of zeolite structure,destruction of isolated Cu^2+site,as well as the formation of highly oxidizing Fe2O3,thus causing deterioration of catalytic performances.
基金supported by the Chinese Academy of Medical Sciences(CAMS)Initiation fund for Medical Science(2016-I2M-1-008)the Drug Innovation Major Project(2018ZX09711-001-005)。
文摘Objective:Parthenolide(PTL)induces anti-tumor effects via the nuclear factor kappa B(NF-κB)signaling pathway.MCL3,a PTL derivative,is a sesquiterpene lactone synthesized by the rearrangement and subsequent oxidation of PTL.The aim of this study was to elucidate the antitumor activity and mechanism of action of MCL3 in glioblastoma(GBM).Materials and Methods:The effects of MCL3 on G422 cell proliferation,apoptosis,invasion,and angiogenesis in vitro were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,flow cytometry,the cell invasion,and tube formation assays.The subcutaneously transplanted G422 xenograft model was used to detect the effect of MCL3 on tumor growth in vivo.Pathological changes were analyzed by immunohistochemical staining.The effects of MCL3 on NF-κB and Stat3 transcriptional activities were examined using a dual-luciferase reporter assay.Protein levels related to the NF-κB/interleukin(IL)-6/Stat3 signaling pathway were determined using western blot analysis.Results:MCL3 inhibited GBM cell proliferation,invasion,and angiogenesis in a concentration-dependent manner.Moreover,MCL3 decreased the transcriptional activities of NF-κB and Stat3.MCL3 suppressed tumor growth in the subcutaneously transplanted G422 xenograft model,while the inhibition rate was 79%in tumor weight at 40.0 mg/kg.MCL3 blocked the NF-κB/IL-6/Stat3 signaling pathway in G422 cells and tumor tissues,resulting in the downregulation of Stat3 target genes related to apoptosis,invasion,etc.,Conclusion:The results show that MCL3 might inhibit G422 GBM growth partly due to the inhibition of the NF-κB/IL-6/Stat3 signaling pathway.