OBJECTIVE To observe effects of tetrahydroxystilbene glucoside(TSG)on behavior,content of dopamine and its metabolites in striatum of Parkinson′s disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydrop...OBJECTIVE To observe effects of tetrahydroxystilbene glucoside(TSG)on behavior,content of dopamine and its metabolites in striatum of Parkinson′s disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)injection.METHODS Mice were randomly divided into control group,model group,TSG low dose(60mg·kg-1)and high dose(120mg·kg-1)groups.The behavior changes of mice were observed by pole test,rotarod test and spontaneous movement test.The tyrosine hydroxylase(TH)positive cells were detected by immunohistochemical method.The content of dopamine(DA)and its metabolites in striatum were determined by HPLC-ECD.RESULTS MPTP model mice showed behavior deficit.The number of TH positive neurons in substantia nigra,the content of dopamine and its metabolites in striatum in model mice decreased significantly compared with control group.TSG ameliorated mice behavior,increased the number of TH positive neurons in the substantia nigra about 18.8%,and elevated the content of dopamine in striatum about 34.5% compared with model mice.CONCLUSION TSG protected dopaminergic neurons against MPTP-induced damage,and may become a candidate drug for prevention and treatment of Parkinson′s disease.展开更多
Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was t...Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was to investigate the effects of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucosides(TSG) on a mitochondrial dysfunction cell model induced by sodium azide and elucidate the underlying mechanisms.Methods:Mitochondrial membrane potential(MMP) was detected by a fluorescence method.Cellular adenosine triphosphate(ATP) level was measured using a firefly luciferase-based kit.Reactive oxygen species(ROS) was detected using dichlorofluorescin diacetate(DCFH-DA).The expression levels of Bcl-2 and Bax were measured by Western blotting assay.Flow cytometry was utilized to measure apoptosis.Results:Pretreatment of TSG(25-200 μmol/L) for 24 h significantly elevated MMP and ATP content,reduced ROS level and Bax/Bcl-2 ratio,and inhibited apoptosis in SH-SY5 Y cells exposed to sodium azide.Conclusion:These results suggest that TSG protects SH-SY5 Y cells against sodium azide-induced mitochondrial dysfunction and apoptosis.These findings are helpful to understand the protective effect of TSG on mitochondria,which are involved in the early stage of AD.展开更多
Objective: Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the mo...Objective: Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms. Methods: Human BMP-2 genetic fragment was amplified and introduced into the pEGFP-C1 vector. After being confirmed by XhoI and BamHI digestion analyses and DNA sequencing, the recombinant pEGFP-C1-BMP-2 plasmid was transfected into COS-7 cells. The influence of BMP-2 on cell migration and cofilin activity was detected by cell scratch assay and Western blotting. Results: The recombinant pEGFP-C1-BMP-2 was effectively expressed in COS-7 cells. An increased phosphorylation of both LIMK1 and cofilin and an enhancement of cell migration were observed in cells with overexpression of BMP-2. Conclusions: A recombinant pEGFP-C1-BMP-2 vector was successfully constructed and overexpression of BMP-2 regulated the activities of the downstream molecules of the Rho GTPase signaling pathway, which might contribute to the enhancement of cell migration.展开更多
基金The project supported by National Natural Science Foundation of China(81273498)the Capital Health Research and Development Foundation(2011-1001-04)
文摘OBJECTIVE To observe effects of tetrahydroxystilbene glucoside(TSG)on behavior,content of dopamine and its metabolites in striatum of Parkinson′s disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)injection.METHODS Mice were randomly divided into control group,model group,TSG low dose(60mg·kg-1)and high dose(120mg·kg-1)groups.The behavior changes of mice were observed by pole test,rotarod test and spontaneous movement test.The tyrosine hydroxylase(TH)positive cells were detected by immunohistochemical method.The content of dopamine(DA)and its metabolites in striatum were determined by HPLC-ECD.RESULTS MPTP model mice showed behavior deficit.The number of TH positive neurons in substantia nigra,the content of dopamine and its metabolites in striatum in model mice decreased significantly compared with control group.TSG ameliorated mice behavior,increased the number of TH positive neurons in the substantia nigra about 18.8%,and elevated the content of dopamine in striatum about 34.5% compared with model mice.CONCLUSION TSG protected dopaminergic neurons against MPTP-induced damage,and may become a candidate drug for prevention and treatment of Parkinson′s disease.
基金supported by the National Natural Science Foundation of China (Nos.81273498, 81341087)National Science and Technology Major Project of China (No.2015ZX09101-016)。
文摘Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was to investigate the effects of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucosides(TSG) on a mitochondrial dysfunction cell model induced by sodium azide and elucidate the underlying mechanisms.Methods:Mitochondrial membrane potential(MMP) was detected by a fluorescence method.Cellular adenosine triphosphate(ATP) level was measured using a firefly luciferase-based kit.Reactive oxygen species(ROS) was detected using dichlorofluorescin diacetate(DCFH-DA).The expression levels of Bcl-2 and Bax were measured by Western blotting assay.Flow cytometry was utilized to measure apoptosis.Results:Pretreatment of TSG(25-200 μmol/L) for 24 h significantly elevated MMP and ATP content,reduced ROS level and Bax/Bcl-2 ratio,and inhibited apoptosis in SH-SY5 Y cells exposed to sodium azide.Conclusion:These results suggest that TSG protects SH-SY5 Y cells against sodium azide-induced mitochondrial dysfunction and apoptosis.These findings are helpful to understand the protective effect of TSG on mitochondria,which are involved in the early stage of AD.
基金Project supported by the National Natural Science Foundation of China(No.81000535)the Zhejiang Provincial Natural Science Foundation of China(No.Y2100508)
文摘Objective: Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms. Methods: Human BMP-2 genetic fragment was amplified and introduced into the pEGFP-C1 vector. After being confirmed by XhoI and BamHI digestion analyses and DNA sequencing, the recombinant pEGFP-C1-BMP-2 plasmid was transfected into COS-7 cells. The influence of BMP-2 on cell migration and cofilin activity was detected by cell scratch assay and Western blotting. Results: The recombinant pEGFP-C1-BMP-2 was effectively expressed in COS-7 cells. An increased phosphorylation of both LIMK1 and cofilin and an enhancement of cell migration were observed in cells with overexpression of BMP-2. Conclusions: A recombinant pEGFP-C1-BMP-2 vector was successfully constructed and overexpression of BMP-2 regulated the activities of the downstream molecules of the Rho GTPase signaling pathway, which might contribute to the enhancement of cell migration.
