Human papillomavirus is an important cancer factor in many cancers. E6E7 is the important viral oncogene and plays an important role. It is known that its function is to regulate p53 and pRb. In this paper, in situdet...Human papillomavirus is an important cancer factor in many cancers. E6E7 is the important viral oncogene and plays an important role. It is known that its function is to regulate p53 and pRb. In this paper, in situdetection was performed to determine the interaction between them;the research provides visual evidence of their interaction. Research methods: In situ PLA reaction was used to detect the relationship between two different protein. Results: The quantity of the HPV18E6 expression is much higher than the expression of HPV18E7 protein in HeLa and EC109 cells;the interaction between p53-E6 and pRb-E7 was clearly observed and this effect could be visualized by this method. Conclusion: Interaction of the HPV18E6 protein combined with p53 protein and HPV18E7 protein combined with pRb protein could be visualized in cell.展开更多
By using mixed-solvents method,five kinds of p-substituted tetraphenylporphyrin compounds[T(p-R)PPH_(2),R=NO_(2),Cl,CH_(3),OCH_(3),OH]were synthesized by the condensation of p-substituted benzaldehyde with pyrrole in ...By using mixed-solvents method,five kinds of p-substituted tetraphenylporphyrin compounds[T(p-R)PPH_(2),R=NO_(2),Cl,CH_(3),OCH_(3),OH]were synthesized by the condensation of p-substituted benzaldehyde with pyrrole in mixed solvents(propionic acid,acetic acid and nitrobenzene),and corresponding ferric complexes[T(p-R)PPFe^(Ⅲ)Cl]were synthesized in dimethylformamide.The above free base porphyrins were obtained in 30%-50%yields,metalation yields were up to 90%and total yields of ferric complexes were 27%-50%.Effects of reactive conditions,solvents and oxidants on yields of free base porphyrins were investigated and the relevant mechanism was discussed.Structures of the above porphyrin complexes were characterized by ultravioletvisible(UV-Vis),infrared(IR)and far infrared(FIR)spectroscopy.展开更多
文摘Human papillomavirus is an important cancer factor in many cancers. E6E7 is the important viral oncogene and plays an important role. It is known that its function is to regulate p53 and pRb. In this paper, in situdetection was performed to determine the interaction between them;the research provides visual evidence of their interaction. Research methods: In situ PLA reaction was used to detect the relationship between two different protein. Results: The quantity of the HPV18E6 expression is much higher than the expression of HPV18E7 protein in HeLa and EC109 cells;the interaction between p53-E6 and pRb-E7 was clearly observed and this effect could be visualized by this method. Conclusion: Interaction of the HPV18E6 protein combined with p53 protein and HPV18E7 protein combined with pRb protein could be visualized in cell.
基金supported by the Project of the National Natural Science Foundation of China(Grant Nos.20776003,20576005)the Key Project of Natural Science Foundation of Beijing(No.2061001).
文摘By using mixed-solvents method,five kinds of p-substituted tetraphenylporphyrin compounds[T(p-R)PPH_(2),R=NO_(2),Cl,CH_(3),OCH_(3),OH]were synthesized by the condensation of p-substituted benzaldehyde with pyrrole in mixed solvents(propionic acid,acetic acid and nitrobenzene),and corresponding ferric complexes[T(p-R)PPFe^(Ⅲ)Cl]were synthesized in dimethylformamide.The above free base porphyrins were obtained in 30%-50%yields,metalation yields were up to 90%and total yields of ferric complexes were 27%-50%.Effects of reactive conditions,solvents and oxidants on yields of free base porphyrins were investigated and the relevant mechanism was discussed.Structures of the above porphyrin complexes were characterized by ultravioletvisible(UV-Vis),infrared(IR)and far infrared(FIR)spectroscopy.
