Metal-organic frameworks(MOFs),a crystalline porous material with a periodic net-work structure formed by the self-assembly of transition metal ions and organic ligands,have been widely applied in various fields due t...Metal-organic frameworks(MOFs),a crystalline porous material with a periodic net-work structure formed by the self-assembly of transition metal ions and organic ligands,have been widely applied in various fields due to their rich composition and structural diversity.Among vari-ous types of MOFs,stimuli-responsive MOFs have gained increasing attention in recent years,because of their broad application in the field of physics,biology,and chemistry.In this review,we analyzed and classified the mechanism of stimulus-response MOFs(pH response,glucose response,GSH response,light response,temperature response)and their applications in drug delivery,adsorption and luminescence functions,magnetization and catalysis functions,probe and sensor.展开更多
Background:AG8,a triterpenoid saponin isolated from Ardisia gigantifolia Stapf.,our previous studies found that AG8 inhibited the proliferation of triple negative breast cancers(TNBCs)by including ROS generation and t...Background:AG8,a triterpenoid saponin isolated from Ardisia gigantifolia Stapf.,our previous studies found that AG8 inhibited the proliferation of triple negative breast cancers(TNBCs)by including ROS generation and triggering mitochondrial apoptotic pathways.Cancer stem cells(CSCs)capable of maintain relatively low levels of ROS,are vulnerable to the interference of redox state.Compounds capable of generating ROS can affect the finely balanced redox state of CSCs and have potency to selectively kill them.Objective:We selected BT-549 which is most sensitive to AG8 to further study the effects of AG8 on hypoxia-induced proliferation,metastasis and CSCs.Methods:The hypoxia condition was simulated by CoCl2 and cell viability assay of BT-549 cells was performed us-ing MTT.Stemness phenotype were identified using breast CSCs marker(CD24^(−)/low/CD44^(+))and mammosphere formation assay.Cell motility was determined via the wound healing assay and transwell migration.Protein levels of HIF1-α,Oct-4,SOX-2,c-MYC were tested by western blotting.Results:In this study,AG8 showed significant cytotoxic effects on BT-549 triple negative breast cancer cells,but the effects were impaired by HIF1 activation.AG8 inhibited the hypoxia-induced migration and invasion of BT-549 cells.Further studies revealed that hypoxia-induced increases of CD44^(+)CD24^(−)/low and mammosphere population were significantly inhibited by AG8 dose-dependently.Moreover,AG8 decreased the Oct-4 and SOX-2 protein expression without affecting HIF1-αand c-MYC.AG8 significantly inhibited BT-549 xenograft tumors growth in BALB/c nude mice comparing with that of the control group.Conclusion:In summary,AG8 inhibited hypoxia-induced cell migration and invasion through stemness regula-tions,indicating novel mechanisms for the antitumor effects of AG8 against triple negative breast cancer.展开更多
To the Editor:Revascularization,regardless of whether it is percutaneous coronary intervention(PCI)or coronary artery bypass grafting(CABG),is still the dominant treatment of severe coronary heart disease(CHD).The lon...To the Editor:Revascularization,regardless of whether it is percutaneous coronary intervention(PCI)or coronary artery bypass grafting(CABG),is still the dominant treatment of severe coronary heart disease(CHD).The long-term clinical outcome of revascularization is unpredictable because of the progression of atherosclerosis continuing after surgery.[1]Many studies and trials have proven that optimal medical therapy(OMT)with or without revascularization is critical for decreasing the incidence of adverse events and improving quality of life.In recent years,patients from China with higher adherence to medical therapy have shown a lower incidence of adverse events at 1 year after PCI.[2]However,there have been no large clinical trials or observational studies for accurate understanding of maintenance of medical strategies in patients with CHD in China.展开更多
Background Snake venom contains a number of components with different pharmacological and biological activities, especially in cancer therapy, and has increasingly become a research focus. This study was designed to i...Background Snake venom contains a number of components with different pharmacological and biological activities, especially in cancer therapy, and has increasingly become a research focus. This study was designed to isolate and purify a novel anti-clotting protein component from the venom of Agkistrodon acutus, and to explore its physico-chemical properties and biological activity. Methods The venom of Agkistrodon was isolated and purified by ion-exchange chromatography on diethylaminoethyl （DEAE）-Sepharose Fast Flow, molecular sieve filtration through Sephadex G75, SP-Sepharose Fast Flow and molecular sieve filtration through Sephadex G50. We detected the activated partial thromboplastin time （APTT） of the eluant to select the anti-clotting protein component of interest. The molecular weight was determined by sodium dodecyl sulfate-polyacrylamid gel electrphoresis （SDS-PAGE） and liquid chromatography. Its protein content was detected by bicinchoninic acid （BCA）. Results SDS-PAGE vertical gel electrophoresis showed that the anticoagulant factor is a tripolymer composed of three proteins whose molecular weights are 25 KDa, 30 KDa and 50 KDa. The factor contains about 65% percent protein. Conclusions A novel anti-clotting protein component was purified by ion-exchange chromatography and molecular sieve filtration from the venom of Agkistrodon acutus and was found to be composed of three kinds of proteins.展开更多
基金supported by the National Natural Science Foundation of China(U20A20388).
文摘Metal-organic frameworks(MOFs),a crystalline porous material with a periodic net-work structure formed by the self-assembly of transition metal ions and organic ligands,have been widely applied in various fields due to their rich composition and structural diversity.Among vari-ous types of MOFs,stimuli-responsive MOFs have gained increasing attention in recent years,because of their broad application in the field of physics,biology,and chemistry.In this review,we analyzed and classified the mechanism of stimulus-response MOFs(pH response,glucose response,GSH response,light response,temperature response)and their applications in drug delivery,adsorption and luminescence functions,magnetization and catalysis functions,probe and sensor.
文摘Background:AG8,a triterpenoid saponin isolated from Ardisia gigantifolia Stapf.,our previous studies found that AG8 inhibited the proliferation of triple negative breast cancers(TNBCs)by including ROS generation and triggering mitochondrial apoptotic pathways.Cancer stem cells(CSCs)capable of maintain relatively low levels of ROS,are vulnerable to the interference of redox state.Compounds capable of generating ROS can affect the finely balanced redox state of CSCs and have potency to selectively kill them.Objective:We selected BT-549 which is most sensitive to AG8 to further study the effects of AG8 on hypoxia-induced proliferation,metastasis and CSCs.Methods:The hypoxia condition was simulated by CoCl2 and cell viability assay of BT-549 cells was performed us-ing MTT.Stemness phenotype were identified using breast CSCs marker(CD24^(−)/low/CD44^(+))and mammosphere formation assay.Cell motility was determined via the wound healing assay and transwell migration.Protein levels of HIF1-α,Oct-4,SOX-2,c-MYC were tested by western blotting.Results:In this study,AG8 showed significant cytotoxic effects on BT-549 triple negative breast cancer cells,but the effects were impaired by HIF1 activation.AG8 inhibited the hypoxia-induced migration and invasion of BT-549 cells.Further studies revealed that hypoxia-induced increases of CD44^(+)CD24^(−)/low and mammosphere population were significantly inhibited by AG8 dose-dependently.Moreover,AG8 decreased the Oct-4 and SOX-2 protein expression without affecting HIF1-αand c-MYC.AG8 significantly inhibited BT-549 xenograft tumors growth in BALB/c nude mice comparing with that of the control group.Conclusion:In summary,AG8 inhibited hypoxia-induced cell migration and invasion through stemness regula-tions,indicating novel mechanisms for the antitumor effects of AG8 against triple negative breast cancer.
基金the Tianjin Science and Technology Committee(CN)(No.16ZXMJSY00080).
文摘To the Editor:Revascularization,regardless of whether it is percutaneous coronary intervention(PCI)or coronary artery bypass grafting(CABG),is still the dominant treatment of severe coronary heart disease(CHD).The long-term clinical outcome of revascularization is unpredictable because of the progression of atherosclerosis continuing after surgery.[1]Many studies and trials have proven that optimal medical therapy(OMT)with or without revascularization is critical for decreasing the incidence of adverse events and improving quality of life.In recent years,patients from China with higher adherence to medical therapy have shown a lower incidence of adverse events at 1 year after PCI.[2]However,there have been no large clinical trials or observational studies for accurate understanding of maintenance of medical strategies in patients with CHD in China.
文摘Background Snake venom contains a number of components with different pharmacological and biological activities, especially in cancer therapy, and has increasingly become a research focus. This study was designed to isolate and purify a novel anti-clotting protein component from the venom of Agkistrodon acutus, and to explore its physico-chemical properties and biological activity. Methods The venom of Agkistrodon was isolated and purified by ion-exchange chromatography on diethylaminoethyl （DEAE）-Sepharose Fast Flow, molecular sieve filtration through Sephadex G75, SP-Sepharose Fast Flow and molecular sieve filtration through Sephadex G50. We detected the activated partial thromboplastin time （APTT） of the eluant to select the anti-clotting protein component of interest. The molecular weight was determined by sodium dodecyl sulfate-polyacrylamid gel electrphoresis （SDS-PAGE） and liquid chromatography. Its protein content was detected by bicinchoninic acid （BCA）. Results SDS-PAGE vertical gel electrophoresis showed that the anticoagulant factor is a tripolymer composed of three proteins whose molecular weights are 25 KDa, 30 KDa and 50 KDa. The factor contains about 65% percent protein. Conclusions A novel anti-clotting protein component was purified by ion-exchange chromatography and molecular sieve filtration from the venom of Agkistrodon acutus and was found to be composed of three kinds of proteins.
