白来航鸡不同来源间充质干细胞的比较研究

【目的】以禽类间充质干细胞(MSCs)为研究对象,探讨不同来源MSCs生物学特性上的差异及其临床应用潜力。【方法】培养白来航鸡4种不同组织器官骨髓(BM)、脂肪(AT)、脐带(UC)和脾脏(S)衍生的MSCs,通过实时荧光定量PCR技术检测细胞表面标记物的表达,采用细胞生长曲线观察种群倍增时间变化,利用噻唑蓝细胞毒性检测法(MTT)确定基质细胞衍生因子1(SDF-1)对不同来源MSCs的细胞毒性,并选用细胞小室检测MSCs体外迁移情况,通过蛋白质印迹法方法确定MSCs体外迁移影响因素,最后选用成骨分化和成脂分化观察不同来源MSCs的体外分化情况。【结果】不同来源的MSCs细胞形态基本一致;细胞表面标记物CD 29、CD 44、CD 71、CD 90和CD 105等基因呈阳性表达;不同来源的MSCs表面标记物的相对表达量不同,CD 29基因的相对表达量基本相同,CD 44基因在AT-MSCs中的相对表达量显著高于BM-MSCs(P<0.05),CD 71基因在UC-MSCs中的相对表达量显著低于BM-MSCs(P<0.05),CD90基因在UC-MSCs和S-MSCs中的相对表达量极显著低于BM-MSCs(P<0.01);CD 105基因在S-MSCs中的相对表达量显著低于BM-MSCs(P<0.05)。4种来源MSCs均有较好的体外生长潜力,其中S-MSCs生产潜伏期最长(40 h)。4种来源MSCs均具有较强的体外迁移能力,S-MSCs中CXC趋化因子受体4(CXCR4)的低表达可能是导致其体外迁移效率较低的原因。4种来源的MSCs均具有体外成骨分化和成脂分化能力,其中BM-MSCs的成骨分化能力较强,S-MSCs的成脂分化能力较强。【结论】白来航鸡4种来源的组织器官均可衍生出MSCs,且均具有良好的体外增殖、体外迁移及体外分化能力。4种MSCs的生物学特性存在差异,具有不同的临床应用潜能,BM-MSCs的综合性能较优,S-MSCs的脂肪诱导分化方面优势明显。本研究加强了对禽类MSCs的了解,对MSCs治疗禽类相关疾病的研究及珍稀禽类的保护具有重要意义。

基于数据挖掘算法的慢病住院费用预测模型分析

目的以研究医保住院费用报销的合理性为目的,分析慢性传染性疾病门诊费用结构、门诊治疗情况及患者静态特征等因素对住院费用的影响,并通过数据挖掘算法构建住院费用的预测模型。方法在构建模型的过程中,分别使用了决策树、Naive Bayes、Adaboost等算法,并比较了这些算法的准确度。结果以上采用的模型算法可以使得预测模型的准确度达到93%以上,能够较好地预测住院费用的合理性。肾衰竭/尿毒症的门诊特病费用与住院费用呈负相关趋势(r=-0.10,P<0.05),高血压、冠心病、糖尿病、脑血管病均呈正相关趋势(r=0.11、0.10、0.12、0.11,P<0.05)。结论根据不同类别疾病的特点,可以调整部分住院治疗项目到门诊,以实现对住院费用的调控。

Piezoelectric nanofoams with the interlaced ultrathin graphene confining Zn–N–C dipoles for efficient piezocatalytic H_(2) evolution under low-frequency vibration

Unique nanofoams consisting of interweaved ultrathin graphene confining Zn–N–C dipoles (ZnNG) are constructed via calcination of Zn-coordinated precursor.Due to the introduction of local polar Zn–N–C configurations,with hypersensitivity for mechanical stress,the piezoelectricity is created on the nonpiezoelectric graphene,and the hierarchical ZnNG exhibits obvious piezocatalytic activity of water splitting for H_(2) production even under mild agitation.The corresponding rate of H_(2) production is about 14.65 μmol g^(-1)h^(-1).It triggers a breakthrough in piezocatalytic H_(2) evolution under low-frequency vibration,and takes a significant step forward for piezocatalysis towards practical applications.Furthermore,the presented concept of confining atomic polar configuration for engineering piezoelectricity would open up new horizon for constructing new-type piezoelectrics based on both piezoelectric and nonpiezoelectric materials.

Laser-assisted Liposuction Combined with Masseter Dissection for Mid-Lower Facial Recontouring

Background Asian women usually view the oval face,with a beautiful curve and clear outline,as elegant.The fat and bloated mid-lower face,with an undefined outline,takes away from what they perceive to be a beautiful appearance.Not only a bone but also soft tissue determine the shape of the mid-lower face.In this article,we introduce a modified surgical method of soft tissue for mid-lower facial recontouring,which can improve the fat and undefined outline of the mid-lower face caused by benign masseter muscle hypertrophy and reduce subcutaneous fat accumulation at the same time.The operative technique is simple and effective with few complications.Methods The surgical treatment of 36 patients who met the adaptation criteria was carried out by laser-assisted liposuction combined with masseter muscle dissection.All patients were followed up postoperatively,and the surgical efficacy was evaluated on the basis of:the comparison of facial contours of patients before and after surgery,the change of masseter muscle thickness detected by B-ultrasound imaging,the satisfaction of patients with postoperative facial contour improvement,and the incidence of complications.Results Between March 1,2018,and August 31,2019,36 patients with indications underwent this procedure.The follow-up lasted 6 to 12 months postoperatively and demonstrated that all patients were satisfied with the postoperative results,of which 33 cases(91.7%)were very satisfied,and 3 cases(8.3%)were satisfied with the treatment and had obvious improvement of the appearance of their mid-lower face.Preoperatively,the average mandibular bigonial breadth was 138.6±8.3 mm,and the average thickness of the masseter muscle was 13.9±0.8 mm;the postoperative breadth was 123.3±7.7 mm,and the thickness was 10.1±0.2 mm.The average reduction of the lower face width and masseter thickness were 15.2±2.1 mm and 3.8±0.1 mm,respectively.No complications occurred among the 36 patients.Conclusion The described method makes a great improvement in reshaping the outline of the mid-lower face,improves the fat and unclear outline of the mid-lower face caused by masseter muscle hypertrophy,and reduces subcutaneous fat accumulation.The procedure is easy to perform and is minimally traumatic,allowing for a quick recovery with few complications.These advantages along with the patients'overall satisfaction with the results make it worthy of clinical application.

联合检测血清指标对老年社区获得性肺炎患者预后的评估价值

目的分析联合检测血清C反应蛋白(CRP)、降钙素原和乳酸对老年社区获得性肺炎(CAP)患者预后的评估价值。方法选取商丘市第一人民医院急诊科95例老年CAP患者为病例组,同期选取商丘市第一人民医院急诊科45例老年体检健康人员为对照组,比较两组血乳酸、降钙素原和CRP的水平,同时比较病例组不同预后状况的患者血乳酸、降钙素原和CRP水平,通过绘制受试者工作特征曲线(ROC)评价CRP、降钙素原和血乳酸单独或联合检测对老年CAP患者预后状况的预测价值。结果与对照组比较,病例组血乳酸、降钙素原和CRP含量均明显升高(t=20.77、26.03,31.27,均P<0.01)。随访12个月,病例组死亡13例(13.68%、13/95)、存活82例(86.32%、82/95),死亡组血乳酸、降钙素原和CRP含量较存活组均明显升高(t=25.56、8.30,13.56,均P<0.01)。曲线ROC分析结果显示,血乳酸、降钙素原和CRP含量预测老年CAP患者预后的敏感度分别为76.92%、84.62%、69.23%,特异度分别为73.17%,78.05%,70.73%,而三者联合检测的敏感度和特异度较单独检测均明显升高,分别为92.31%、89.02%。结论老年CAP患者血乳酸、降钙素原和CRP含量明显升高,通过联合检测这三项血清学指标可提高对患者预后状况的预测价值,具有重要的临床意义。

白来航鸡胃部间充质干细胞的分离培养及鉴定

该研究采用16日龄白来航鸡胃部组织分离培养胃部间充质干细胞(G-MSCs),观察G-MSCs生长特性,为鸡体外研究方法的开发奠定实验基础。细胞生长曲线表明,白来航鸡G-MSCs在体外培养条件下具有良好的扩增能力,且P3代细胞的增殖能力更好。通过反转录聚合酶链式反应(RT-PCR)确定了细胞表面标记物的表达情况,CD29、CD44、CD73、CD71、CD90、CD105呈阳性表达,CD31、CD45、CD34呈阴性表达。成骨诱导实验确定了G-MSCs具有成骨分化和成脂肪分化能力。利用微粒体培养进行成骨分化研究,结果表明微粒体培养能够诱导形成成骨组织块,但成骨诱导率不高。最后利用细胞划痕发现,G-MSCs具有体外迁移能力。该研究成功地从白来航鸡胃部组织中分离培养出了G-MSCs并鉴定了其生物学特性,为鸡G-MSCs的研究奠定了基础,为利用G-MSCs进行胃癌等疾病的治疗提供了新思路。

Sulfonated Polyimides Containing 1,2,4-Triazole Groups for Proton Exchange Membranes

A series of sulfonated polyimide copolymers as novel proton exchange materials were synthesized by the polycondensation of 1,4,5,8-naphthalene-tetracarboxylic dianhydride （NTDA）, sulfonated diamine based on pyridine group and diamine containing N-phenyl-1,2,4-triazole moiety. Flexible, transparent and tough membranes with high thermal stability and good mechanical properties were obtained. They exhibited good stability in boiling water and Fenton＇s reagent at 80 ℃. More interestingly, a nonlinear relationship between proton conductivities of the resulting membranes and the degree of sulfonation （DS） was observed. The membrane with 50% DS exhibited the maximum proton conductivity, which was due to the combinational contributions of sulfonic acid and N-pheny-1,2,4-triazole groups. Thus, the N-phenyl-l,2,4- triazole moiety in this study not only can depress water absorption but also increase proton conductivity, especially at low DS.

Self-Powered Room-Temperature Ethanol Sensor Based on Brush-Shaped Triboelectric Nanogenerator

Highly sensitive ethanol sensors have been widely utilized in environmental protection,industrial monitoring,and drink-driving tests.In this work,a fully self-powered ethanol detector operating at room temperature has been developed based on a triboelectric nanogenerator(TENG).The gas-sensitive oxide semiconductor is selected as the sensory component for the ethanol detection,while the resistance change of the oxide semiconductor can well match the“linear”region of the load characteristic curve of TENG.Hence,the output signal of TENG can directly reveal the concentration change of ethanol gas.An accelerator gearbox is applied to support the operation of the TENG,and the concentration change of ethanol gas can be visualized on the Liquid Crystal Display.This fully self-powered ethanol detector has excellent durability,low fabrication cost,and high selectivity of 5 ppm.Therefore,the ethanol detector based on TENG not only provides a different approach for the gas detection but also further demonstrates the application potential of TENG for various sensory devices.