Histopathological characteristics of needle core biopsy and surgical specimens from patients with solitary hepatocellular carcinoma or intrahepatic cholangiocarcinoma

BACKGROUND Pathological manifestations of hepatic tumours are often associated with prognosis. Although surgical specimens(SS) can provide more information,currently, pre-treatment needle core biopsy(NCB) is increasingly showing important value in understanding the nature of liver tumors and even in diagnosis and treatment decisions. However, the concordance of the clinicopathological characteristics and immunohistochemical(IHC) staining between NCB and SS from patients with hepatic tumours were less concerned.AIM To introduce a more accurate method for interpreting the IHC staining results in order to improve the diagnostic value of hepatic malignancy in NCB samples.METHOD A total of 208 patients who underwent both preoperative NCB and surgical resection for hepatocellular carcinoma(HCC) or intrahepatic cholangiocarcinomaRESULTS Morphologically, the presence of compact tumour nests or a cord-like structure in NCB was considered the primary cause of misdiagnosis of HCC from ICC. The kappa statistic showed a moderate agreement in histomorphology(k = 0.504) and histological grade(k = 0.488) between NCB and SS of the tumours. A 4-tier(+++,++, +, and-) scoring scheme that emphasized the focal neoplastic cell immunoreactivity of tumour cells revealed perfect concordance of CK19, GPC3 and HepPar1 between NCB and SS(k = 0.717; k = 0.768; k = 0.633). Furthermore,with the aid of a binary classification derived from the 4-tier score, a high concordance was achieved in interpreting the IHC staining of the three markers between NCB and final SS(k = 0.931; k = 0.907; k = 0.803), increasing the accuracy of NCB diagnosis C(k = 0.987; area under the curve = 0.997, 95%CI: 0.990-1.000; P< 0.001).CONCLUSION These findings imply that reasonable interpretation of IHC results in NCB is vital for improving the accuracy of tumour diagnosis. The simplified binary classification provides an easy and applicable approach.

Going beyond the limit of an LCD’s color gamut

In this study,we analyze how a backlight’s peak wavelength,full-width at half-maximum(FWHM),and color filters affect the color gamut of a liquid crystal display(LCD)device and establish a theoretical limit,even if the FWHM approaches 1 nm.To overcome this limit,we propose a new backlight system incorporating a functional reflective polarizer and a patterned half-wave plate to decouple the polarization states of the blue light and the green/red lights.As a result,the crosstalk between three primary colors is greatly suppressed,and the color gamut is significantly widened.In the experiment,we prepare a white-light source using a blue light-emitting diode(LED)to pump green perovskite polymer film and red quantum dots and demonstrate an exceedingly large color gamut(95.8%Rec.2020 in Commission internationale de l'éclairage(CIE)1931 color space and 97.3%Rec.2020 in CIE 1976 color space)with commercial high-efficiency color filters.These results are beyond the color gamut limit achievable by a conventional LCD.Our design works equally well for other light sources,such as a 2-phosphorconverted white LED.