Objective: To study the effects of endoplasmic reticulum stress in cartilage tissue of knee osteoarthritis (OA) on the secretion of inflammation molecules and bone metabolism molecules. Methods: The patients who recei...Objective: To study the effects of endoplasmic reticulum stress in cartilage tissue of knee osteoarthritis (OA) on the secretion of inflammation molecules and bone metabolism molecules. Methods: The patients who received joint replacement in our hospital due to knee osteoarthritis between June 2013 and February 2017 were enrolled as the OA group, and the patients who received knee replacement in our hospital due to trauma during the same period were enrolled as the control group. During the operation, articular cartilage tissue was collected to measure the expression of endoplasmic reticulum stress genes as well as the secretion of inflammation molecules and bone metabolism molecules. Results: PRKR-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme 1 (IRE1), activating transcription factor 6 (ATF6), CCAAT/enhancer-binding protein homologous protein (CHOP) and cysteinyl aspartate specific proteinase-12 (caspase-12) mRNA expression as well as interleukin-1β (IL-1β), IL-6, CXC chemokine ligand 12 (CXCL12), Chemerin, human cartilage glycoprotein-39 (YKL-40), matrix metalloproteinase 13 (MMP13) and receptor activator for nuclear factor-κB ligand (RANKL) secretion in articular cartilage of OA group were obviously higher than those of control group whereas osteoprotegerin (OPG), osteocalcin (OC), collagen-I (COL-I) and COL-III secretion were obviously lower than those of control group, and PERK, IRE1, ATF6, CHOP and Caspase-12 mRNA expression were positively correlated with IL-1β, IL-6, CXCL12, Chemerin,YKL-40, MMP13 and RANKL, and negatively correlated with OPG, OC, COL-I and COL-III. Conclusion: The over-activation of endoplasmic reticulum stress in knee OA cartilage tissue is related to the inflammatory response activation and bone metabolism disorder.展开更多
文摘Objective: To study the effects of endoplasmic reticulum stress in cartilage tissue of knee osteoarthritis (OA) on the secretion of inflammation molecules and bone metabolism molecules. Methods: The patients who received joint replacement in our hospital due to knee osteoarthritis between June 2013 and February 2017 were enrolled as the OA group, and the patients who received knee replacement in our hospital due to trauma during the same period were enrolled as the control group. During the operation, articular cartilage tissue was collected to measure the expression of endoplasmic reticulum stress genes as well as the secretion of inflammation molecules and bone metabolism molecules. Results: PRKR-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme 1 (IRE1), activating transcription factor 6 (ATF6), CCAAT/enhancer-binding protein homologous protein (CHOP) and cysteinyl aspartate specific proteinase-12 (caspase-12) mRNA expression as well as interleukin-1β (IL-1β), IL-6, CXC chemokine ligand 12 (CXCL12), Chemerin, human cartilage glycoprotein-39 (YKL-40), matrix metalloproteinase 13 (MMP13) and receptor activator for nuclear factor-κB ligand (RANKL) secretion in articular cartilage of OA group were obviously higher than those of control group whereas osteoprotegerin (OPG), osteocalcin (OC), collagen-I (COL-I) and COL-III secretion were obviously lower than those of control group, and PERK, IRE1, ATF6, CHOP and Caspase-12 mRNA expression were positively correlated with IL-1β, IL-6, CXCL12, Chemerin,YKL-40, MMP13 and RANKL, and negatively correlated with OPG, OC, COL-I and COL-III. Conclusion: The over-activation of endoplasmic reticulum stress in knee OA cartilage tissue is related to the inflammatory response activation and bone metabolism disorder.
