In order to demonstrate the relationship between methylation of fragile histidine triad(FHIT)and T-cadherin/H-cadherin(CDH13)genes and liver cancer,the methylation status of FHIT and CDH13 was detected in healthy indi...In order to demonstrate the relationship between methylation of fragile histidine triad(FHIT)and T-cadherin/H-cadherin(CDH13)genes and liver cancer,the methylation status of FHIT and CDH13 was detected in healthy individuals and in Mongolian and Han patients with liver cancer.The phenol-chloroform method was used to extract genomic DNA.The methylation specific polymerase chain reaction method was applied to detect the methylation status of FHIT and CDH13.The relationship between smoking and alcohol consumption and gene(FHIT and CDH13)methylation was analyzed.There was significant difference in methylation rate of FHIT(72.67%,34.67%)and CDH13(72.0%,28.0%)between liver cancer patients and healthy individuals of Mongolian descent(P<0.05),as well as that of FHIT(68%,30.67%)and CDH13(64%,26%)between liver cancer patients and healthy individuals of Han individuals(P<0.05).There was also a relationship between smoking and drinking and the methylation of FHIT and CDH13(P<0.05).Thus,the methylation of FHIT and CDH13 had a relationship with liver cancer incidence.Smoking and alcohol ingestion may promote the methylation of FHIT and CDH13.展开更多
基金This work was supported by grants from the Major Scientific Project of the Affiliated Hospital of Inner Mongolia Medical University(No.NYFY ZD009)National Natural Science Foundation of China(No.81760676).
文摘In order to demonstrate the relationship between methylation of fragile histidine triad(FHIT)and T-cadherin/H-cadherin(CDH13)genes and liver cancer,the methylation status of FHIT and CDH13 was detected in healthy individuals and in Mongolian and Han patients with liver cancer.The phenol-chloroform method was used to extract genomic DNA.The methylation specific polymerase chain reaction method was applied to detect the methylation status of FHIT and CDH13.The relationship between smoking and alcohol consumption and gene(FHIT and CDH13)methylation was analyzed.There was significant difference in methylation rate of FHIT(72.67%,34.67%)and CDH13(72.0%,28.0%)between liver cancer patients and healthy individuals of Mongolian descent(P<0.05),as well as that of FHIT(68%,30.67%)and CDH13(64%,26%)between liver cancer patients and healthy individuals of Han individuals(P<0.05).There was also a relationship between smoking and drinking and the methylation of FHIT and CDH13(P<0.05).Thus,the methylation of FHIT and CDH13 had a relationship with liver cancer incidence.Smoking and alcohol ingestion may promote the methylation of FHIT and CDH13.
