Improvement of Binocular Reconstruction Algorithm for Measuring 3D Pavement Texture Using a Single Laser Line Scanning Constraint

The dense and accurate measurement of 3D texture is helpful in evaluating the pavement function.To form dense mandatory constraints and improve matching accuracy,the traditional binocular reconstruction technology was improved threefold.First,a single moving laser line was introduced to carry out global scanning constraints on the target,which would well overcome the difficulty of installing and recognizing excessive laser lines.Second,four kinds of improved algorithms,namely,disparity replacement,superposition synthesis,subregion segmentation,and subregion segmentation centroid enhancement,were established based on different constraint mechanism.Last,the improved binocular reconstruction test device was developed to realize the dual functions of 3D texture measurement and precision self-evaluation.Results show that compared with traditional algorithms,the introduction of a single laser line scanning constraint is helpful in improving the measurement’s accuracy.Among various improved algorithms,the improvement effect of the subregion segmentation centroid enhancement method is the best.It has a good effect on both overall measurement and single pointmeasurement,which can be considered to be used in pavement function evaluation.

多胺对大鼠缺氧-复氧心肌细胞内钙的影响

目的:观察外源性低浓度多胺对大鼠缺氧-复氧心肌细胞钙超载的影响。方法:酶解分离大鼠心室 肌细胞,用正常氧合Tyrode液灌流8 min,换为缺氧液灌流32 min,再转换为正常氧合Tyrode液灌流8 min,复制心肌 缺氧-复氧模型。分别在缺氧前给予精胺,缺氧-复氧后给予精胺、精脒、腐胺。应用激光扫描共聚焦显微镜 (LSCM)连续观察细胞内钙荧光强度的动态变化。结果:精胺(1 mol/L)对正常静息状态下大鼠心室肌[Ca2+]i无影 响。缺氧前给予精胺,可取消复氧引起的心肌[Ca2+]i增高;缺氧-复氧后给予精胺、精脒、腐胺对缺氧-复氧引起 [Ca2+]i升高也有不同程度的降低作用,其中以精胺的作用最强。复氧后给予精胺降低缺氧-复氧[Ca2+]i升高的 作用小于缺氧前给予。结论:缺氧前给予精胺可拮抗缺氧-复氧心肌细胞钙超载发生;复氧后给予精胺可使缺氧- 复氧心肌细胞钙超载减轻,但其作用力度不如缺氧前给药。多胺拮抗缺氧-复氧心肌细胞钙超载的作用以精胺> 精脒>腐胺的顺序递减。

超短期胰岛素强化治疗对住院T2DM患者胰岛β细胞功能的影响

目的探讨超短期多次胰岛素皮下注射(MDI)对2型糖尿病(T2DM)患者胰岛β细胞的临床疗效。方法选取2018年1月—2019年12月就诊于安徽医科大学第四附属医院内分泌与代谢病科的住院T2DM患者69例。比较多次胰岛素皮下注射治疗前后胰岛β细胞功能[HOMA-β(C-P)In]变化。根据糖化血红蛋白(HbA1c)和体重指数(BMI)分为为高糖化组、低糖化组和超重组、非超重组。比较各组多次胰岛素皮下注射治疗前后HOMA-β(C-P)ln水平及其他临床指标变化,并探讨HOMA-β(C-P)In差值和其他临床指标的相关性。结果T2DM患者多次胰岛素皮下注射治疗后FBG、FC-PIn和HOMAIR(C-P)In较注射前下降(P<0.05),HOMA-β(C-P)In较注射前升高(P<0.05)。高糖化组患者多次胰岛素皮下注射前后FBG和HOMA-β(C-P)In差值水平较低糖化组高(P<0.05),不同HbA1c组患者多次胰岛素皮下注射前后其余指标比较,差异无统计学意义(P>0.05)。非超重组患者多次胰岛素皮下注射前后FC-Pln和HOMA-β(C-P)In差值水平FC-PIn差值较超重组高(P<0.05),HOMA-β(C-P)In差值较超重组低。不同BMI组患者多次胰岛素皮下注射前后其余指标比较,差异无统计学意义(P>0.05)。Pearson相关性分析显示,HOMA-β(C-P)ln差值水平与多次胰岛素皮下注射前FBG水平、HOMA-IR(C-P)In水平、HbA1c水平及多次胰岛素皮下注射后HOMA-IR(C-P)In水平、HOMA-β(C-P)In水平呈正相关(r=0.570、0.0331、0.237、0.307、0.560,P<0.05);与多次胰岛素皮下注射后FBG水平、多次胰岛素皮下注射前HOMA-β(C-P)In水平呈负相关(r=-0.495、-0.400,P<0.05)。结论多次胰岛素皮下注射治疗改善住院T2DM患者胰岛β细胞功能,且胰岛β细胞恢复和多种临床指标密切相关。

Single nucleotide polymorphism in DNA methyltransferase 3B promoter and its association with gastric cardiac adenocarcinoma in North China

AIM: To investigate the association between single nucleotide polymorphism (SNP)in promoter of the DNA methyltransferase 3B (DNNT3B) gene and risk for development and lymphatic metastasis of gastric cardiac adenocarcinoma (GCA).METHODS: The hospital based case-control study included 212 GCA patients and 294 control subjects without overt cancer. The DNMT3B SNP was genotyped by PCR and restriction fragment length polymorphism (RFLP) analysis. RESULTS: The C/C genotype was not detected in both GCA patients and controls. In control subjects, the frequency of T/T and C/T genotypes was 94.9% and 5.1% respectively,and that of T and C alleles was 97.4% and 2.6%, respectively.The genotype and allelotype distribution in the GCA patients was not significantly different from that in controls (P = 0.34 and 0.33, respectively). When stratified by smoking status and family history of upper gastrointestinal cancer, significant difference in the genotype distribution was not observed between GCA patients and controls. The distribution of DNMT3Bgenotypes in GCA patients with or without lymphatic metastasis did not show significant difference (P = 0.42). CONCLUSION: The distribution of DNMT3BSNP in North China is distinct from that in Caucasians. Although this SNP has been associated with susceptibility to lung, head, neck and breast cancer, it may not be used as a stratification marker to predict susceptibility and lymphatic metastasis of GCA, at least in the population of North China.

No association of the matrix metalloproteinase 1 promoter polymorphism with susceptibility to esophageal squamous cell carcinoma and gastric cardiac adenocarcinoma in northern China

AIM: To investigate association of the 2G or 1G single nudeotide polymorphism (SNP)in matrix metalloproteinase 11(MMP1)promoter with susceptibility to esophageal squam-ous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in a population of North China.METHODS: MMP1 promoter SNP was genotyped by polymerase-chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis in 417 cancer patients (234ESCC and 183 GCA) and 350 healthy controls.RESULTS: The genotype frequencies of the MMP1promoter SNP in healthy controls were 55.4% (2G/2G),30% (1G/2G) and 14.6% (1G/1G), respectively. The genotype and allelotype distribution in ESCC and GCA patients was not significantly different from that in healthy controls (all Pvalues were above 0.05). Compared with the 1G/1G genotype, neither the 2G/2G nor in combination with the 1G/2G genotype significantly modified the risk of developing ESCC and GCA, the adjusted odds ratio was 1.28 (95%CI = 0.78-2.09), 1.23 (95%CI = 0.38-2.05)in ESCC and 1.39 (95%CI = 0.80-2.41), 1.34 (95%CI =0.74-2.40) in GCA, respectively. When stratified by smoking status and family history of upper gastrointestinal cancer, the 2G/2G genotype alone or in combination with the 1G/2G genotype also did not show any significant influence on the risk of ESCC and GCA development. In addition, influence of the MMP1 SNP on lymphatic metastasis in ESCC and GCA was also not obs-erved.CONCLUSION: The 2G or 1G SNP in the MMP1 promoter might not modify the risk of ESCC and GCA development and might not be used as a stratification marker to predict the potential of lymphatic metastasis in these two tumor types.

Epoxide hydrolase Tyr113 His polymorphism is not associated with susceptibility to esophageal squamous cell carcinoma in population of North China

AIM: To investigate the possible association of microsomal epoxide hydrolase ( mEH) Tyr113 His polymorphism with susceptibility to esophageal squamous cell carcinoma (ESCC) in a population of North China.METHODS: The mEH-Tyr113 His genotypes were determined by polymerase-chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis in 257 patients with esophageal squamous cell carcinoma (ESCC) and 252 healthy subjects as a control group.RESULTS: The frequencies for Tyr and His alleles were 44.2 %, 55.8 % in ESCC patients, and 44.0 % and 56.0 %in healthy subjects, respectively. No statistic difference in allele distribution was observed between ESCC patients and controls (x2=0.008, P=0.929). The overall genotype distribution difference was not observed between cancer cases and controls (x2=2.116, P=-0.347). Compared with Tyr/Tyr genotype, neither His/His genotype nor in combination with Tyr/His genotype significantly modified the risk of the development of ESCC, the adjusted odds ratio was 1.076 (95 % CI=0.850-1.361) and 0.756 (95 % CI=0.493-1.157),respectively. When stratified for sex, age, smoking status and family history of upper gastrointestinal cancer, His/His genotype alone or in combination with Tyr/His genotype also did not show any significant influence on the risk of developing ESCC.CONCLUSION: MEH Tyr113His polymorphism may not be used as a stratification marker in screening individuals at a high risk of ESCC.

大鼠心肌钙敏感受体蛋白的表达及其与心肌细胞凋亡的关系

目的观察大鼠心肌组织和心肌细胞钙敏感受体(CaSR)蛋白表达与心肌细胞凋亡及相关信号传导途径的关系。方法Western blot检测心肌CaSR蛋白和ERK1/2、BCl2及Caspase3的表达。免疫荧光和流式细胞仪观察细胞凋亡。结果心肌组织和心肌细胞表面可检测到CaSR蛋白的表达。CaSR激动剂氯化钆(GdCl3)能促进心肌细胞凋亡,并使ERK1/2的磷酸化增加、BCl2表达及Caspase3活化。加入酪氨酸蛋白激酶阻断剂PD98059后,心肌细胞凋亡率增加,ERK1/2磷酸化降低,BCl2表达消失,Caspase3活化增强。结论CaSR蛋白在心肌细胞膜表面表达,其激活通过Caspase 3活化,促进心肌细胞凋亡。同时亦可激活酪氨酸蛋白激酶途径。