Factors affecting micro-graft in vitro were studied in persimmon lines of Jiro,Nishimarawase,Zenjiomaru,Okitsu-20,Xinqiu,Maekawa Jiro and Youhou. The results indicated that higher survival rate was obtained when using...Factors affecting micro-graft in vitro were studied in persimmon lines of Jiro,Nishimarawase,Zenjiomaru,Okitsu-20,Xinqiu,Maekawa Jiro and Youhou. The results indicated that higher survival rate was obtained when using the plantlets sub-cultured for 30 d with vigorous adventitious shoots. The best moisturepreserving material was the tampon ＋ parafilm. The optimal medium was（ 1/2 N） MS ＋ BA 3. 0 mg/L ＋ IAA 0. 1 mg/L ＋ sugar 30. 0 g/L ＋ agar 6. 0 g/L,and the graft survival rate was up to 40%.展开更多
Citrus fruits produced in China are often affected by granulation.Granulation is an altered physiological state of citrus fruits occurring usually before harvest but whose underlying mechanisms remain elusive.In this ...Citrus fruits produced in China are often affected by granulation.Granulation is an altered physiological state of citrus fruits occurring usually before harvest but whose underlying mechanisms remain elusive.In this study,cDNA-AFLP technology enabled the identification of 116 granulation-associated genes in pummelo(C.grandis)juice sacs.Differentially expressed transcript-derived fragments(TDFs)were shown to be mainly involved in biological regulation and signal transduction,carbohydrate and energy metabolism,nucleic acid,protein metabolism,stress responses,and cell metabolism.Therefore,granulation in pummelo juice sacs seems to involve the following alterations:(1)changes in hormone levels;(2)activation of metabolic pathways related to ATP and sugar synthesis to produce more energy;(3)nucleic acid accumulation and increased protein degradation;(4)activation of stress-responsive metabolic pathways;(5)accelerated juice sac senescence.Our findings provide an overview of differential responses occurring at the transcriptional level in granulated juice sacs,thus revealing new insights into the adaptive mechanisms underlying this altered physiological state in‘Guanximiyou'pummelo(C.grandis)juice sacs.展开更多
基金Supported by Key Scientific Research Project of Colleges and University in Henan Province(17A210013)Fundamental and Advanced Technical Research Program of Department of Science and Technology of Henan Province(122300410133)College Students’Innovation and Enterpreneurship Training Program of Henan Institute of Technology(2014CX047)
文摘Factors affecting micro-graft in vitro were studied in persimmon lines of Jiro,Nishimarawase,Zenjiomaru,Okitsu-20,Xinqiu,Maekawa Jiro and Youhou. The results indicated that higher survival rate was obtained when using the plantlets sub-cultured for 30 d with vigorous adventitious shoots. The best moisturepreserving material was the tampon ＋ parafilm. The optimal medium was（ 1/2 N） MS ＋ BA 3. 0 mg/L ＋ IAA 0. 1 mg/L ＋ sugar 30. 0 g/L ＋ agar 6. 0 g/L,and the graft survival rate was up to 40%.
基金supported by the National Natural Science Foundation of China(NSFC,32002022)Modern Agro-Industry Technology Research System(CARS-26).
文摘Citrus fruits produced in China are often affected by granulation.Granulation is an altered physiological state of citrus fruits occurring usually before harvest but whose underlying mechanisms remain elusive.In this study,cDNA-AFLP technology enabled the identification of 116 granulation-associated genes in pummelo(C.grandis)juice sacs.Differentially expressed transcript-derived fragments(TDFs)were shown to be mainly involved in biological regulation and signal transduction,carbohydrate and energy metabolism,nucleic acid,protein metabolism,stress responses,and cell metabolism.Therefore,granulation in pummelo juice sacs seems to involve the following alterations:(1)changes in hormone levels;(2)activation of metabolic pathways related to ATP and sugar synthesis to produce more energy;(3)nucleic acid accumulation and increased protein degradation;(4)activation of stress-responsive metabolic pathways;(5)accelerated juice sac senescence.Our findings provide an overview of differential responses occurring at the transcriptional level in granulated juice sacs,thus revealing new insights into the adaptive mechanisms underlying this altered physiological state in‘Guanximiyou'pummelo(C.grandis)juice sacs.
