Signal transduction across lipid bilayers is of profound importance in biological processes.In biological systems,natural enzymes mediate biochemical effects by binding to substrates and facilitating the conversion of...Signal transduction across lipid bilayers is of profound importance in biological processes.In biological systems,natural enzymes mediate biochemical effects by binding to substrates and facilitating the conversion of external signals into physiological responses.Sequential transmission of biological signals from one enzyme to the next promotes signal transduction with feedforward and feedback mechanisms.Reconstructing these processes in an artificial system provides potential applications and offers a new way to understand fundamental biological processes in depth.However,the design of artificial signal transduction systems regulated by artificial enzyme receptors in a predictable and intelligent manner remains a challenge.Herein,benefiting from the polarity-regulated characteristics of Se-containing compounds with artificial glutathione peroxidase(GPx)activity,we constructed an artificial transmembrane signaling receptor with a Se-containing GPx-like recognition head group,a membrane-anchoring group,and a pre-enzyme end group.The artificial supramolecular signal transduction system containing such signal transduction receptors extends the range of signaling systems based on enzyme regulation,which provides a new way to study natural signal processes in cells and artificially regulated biological processes.展开更多
Protein assembly is the structural basis for the collaboration between proteins to accomplish life activities due to its realization of the domain-limited and precise spatial arrangement of proteins.Therefore,artifici...Protein assembly is the structural basis for the collaboration between proteins to accomplish life activities due to its realization of the domain-limited and precise spatial arrangement of proteins.Therefore,artificial manipulation of protein selfassembly has profound implications in areas such as exploring the mysteries of life and developing biomaterials.In this review,we not only summarize the classical assembly strategies and the structures of exquisite protein assemblies,but also aim to generalize the flexible and controllable assembly tools and the“interaction”between the assembled protein-based materials and the external environment.On the basis of this,the application,challenges and further development of protein assembly in biology are reviewed.展开更多
基金This work was supported by the National Key Research and Development Program of China(Nos.2020YFA0908500 and 2018YFA0901600)the National Natural Science Foundation of China(No.22161142015).
文摘Signal transduction across lipid bilayers is of profound importance in biological processes.In biological systems,natural enzymes mediate biochemical effects by binding to substrates and facilitating the conversion of external signals into physiological responses.Sequential transmission of biological signals from one enzyme to the next promotes signal transduction with feedforward and feedback mechanisms.Reconstructing these processes in an artificial system provides potential applications and offers a new way to understand fundamental biological processes in depth.However,the design of artificial signal transduction systems regulated by artificial enzyme receptors in a predictable and intelligent manner remains a challenge.Herein,benefiting from the polarity-regulated characteristics of Se-containing compounds with artificial glutathione peroxidase(GPx)activity,we constructed an artificial transmembrane signaling receptor with a Se-containing GPx-like recognition head group,a membrane-anchoring group,and a pre-enzyme end group.The artificial supramolecular signal transduction system containing such signal transduction receptors extends the range of signaling systems based on enzyme regulation,which provides a new way to study natural signal processes in cells and artificially regulated biological processes.
基金National Key R&D Program of China,Grant/Award Numbers:2020YFA0908500,2018YFA0901600National Natural Science Foundation of China,Grant/Award Numbers:22161142015,22001054,22075065,22201058+2 种基金Medical and Health Research Project of Zhejiang Province,Grant/Award Number:2020KY239Hangzhou Overseas High-level Talent(Teams)Innovation and Entrepreneurship ProgramScientific Research Start-up Foundation of Hangzhou Normal University,Grant/Award Numbers:2019QDL026,2019QDL025,2019QDL024。
文摘Protein assembly is the structural basis for the collaboration between proteins to accomplish life activities due to its realization of the domain-limited and precise spatial arrangement of proteins.Therefore,artificial manipulation of protein selfassembly has profound implications in areas such as exploring the mysteries of life and developing biomaterials.In this review,we not only summarize the classical assembly strategies and the structures of exquisite protein assemblies,but also aim to generalize the flexible and controllable assembly tools and the“interaction”between the assembled protein-based materials and the external environment.On the basis of this,the application,challenges and further development of protein assembly in biology are reviewed.
