The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacte...The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacterium paragal-linarum occurred in the Qinling area of China,resulting in clinical symptoms of facial swelling in several bird species,including Golden pheasant,Temminck's tragopan,and Peafowls,and three Golden pheasants died due to prolonged infection.Specific PCR results confrmed the presence of the pathogen in the infected birds.The report describes the clinical symptoms and pathological changes observed in the affected birds,as well as the isolation and identification of Avibacterium paragallinarum.Whole-genome sequencing and phylogenetic anal sis y were performed,and this is the frst report of inter-and intra-species transmission of infectious coryza among wild birds in China.展开更多
Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root o...Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.展开更多
基金This study has been supported by Shaanxi Special Fund Project of Giant Panda International Cooperation(Panda Protection Letter[2019]No.93)the Project of Course Case Library Construction(Grant No.Z1050221011).
文摘The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacterium paragal-linarum occurred in the Qinling area of China,resulting in clinical symptoms of facial swelling in several bird species,including Golden pheasant,Temminck's tragopan,and Peafowls,and three Golden pheasants died due to prolonged infection.Specific PCR results confrmed the presence of the pathogen in the infected birds.The report describes the clinical symptoms and pathological changes observed in the affected birds,as well as the isolation and identification of Avibacterium paragallinarum.Whole-genome sequencing and phylogenetic anal sis y were performed,and this is the frst report of inter-and intra-species transmission of infectious coryza among wild birds in China.
基金This research was based on the‘Functional food research and development of Tetrastigma hemsleyanum’funded by the Shanghai Zhengyue Enterprise Management Co.,Ltd.and the Science and Technology Department of Zhejiang Province(No.2018C02045),China.
文摘Tetrastigma hemsleyanum(T.hemsleyanum)has been regarded as an anticancer food in China.However,its corresponding mechanisms remains unclear.Thus,in this study,the antitumor activity of flavones-rich fraction of root of T.hemsleyanum(FRTH)was investigated in vitro and in vivo.The results indicated that FRTH could inhibit the proliferation and migration of human hepatoellular carcinomas(HepG2)cells in vitro by the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway.FRTH could increase the level of reactive oxygen species and change the mitochondrial membrane potential in HepG2 cells.In addition,FRTH treatment(300 mg/kg and 600 mg/kg,body weight)significantly suppressed tumor growth on HepG2 tumor-bearing nude mice.Besides,immunohistochemistry assays and western blotting revealed that FRTH enhanced the expression level of Bcl-2 associated X protein/B-cell lymphoma-2(Bax/Bcl-2),cytochrome C,caspase-3,caspase-9,and cleaved-caspase-3,and downregulated the expression level of platelet endothelial cell adhesion molecule-1(CD31),ki67,and vascular endothelial growth factor(VEGF)factor in HepG2 tumor-bearing mice.Our study suggests T.hemsleyanum as a kind of promising candidate medicine for liver cancer treatment.
