Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to...Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological processes.Here,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identifying protein interaction networks as well as cell–cell communications in living systems.展开更多
基金funding from the National Natural Science Foundation of China(grant nos.21937001,22222701,22137001,91957101,and 22077004)the Ministry of Science and Technology of China(grant nos.2022YFA1304700,2019YFA0904201,and 2021YFA1302603)Beijing Natural Science Foundation(grant no.Z200010).
文摘Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological processes.Here,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identifying protein interaction networks as well as cell–cell communications in living systems.
