Earthworms promote greater richness and abundance in the emergence of plant species across a grassland-forest ecotone

Aims Chalk grasslands are subject to vegetation dynamics that range from species-rich open grasslands to tall and encroached grasslands,and woods and forests.In grasslands,earthworms impact plant communities and ecosystem functioning through the modification of soil physical,chemical and microbiological properties,but also through their selective ingestion and vertical transportation of seeds from the soil seed bank.Laboratory experiments showed that seed-earthworm interactions are species specific,but little is known on the impact of seed-earthworm interactions in the field.The overall aim of this study was to better understand seed-earthworm interactions and their impact on the plant community.First we analyzed the composition of seedlings emerging from casts after earthworm ingestion.Then we compared seedling composition in casts to the plant composition of emerging seedlings from the soil and of the aboveground vegetation along four stages of the secondary succession of chalk grasslands.Methods Four stages of the secondary succession of a chalk grassland—from open sward to woods—were sampled in Upper Normandy,France,in February 2010.Within each successional stage(×3 replicates),we sampled the standing vegetation,soil seed bank at three soil depths(0-2,2-5 and 5-10 cm)and earthworm surface casts along transects.Soil and cast samples were water sieved before samples were spread onto trays and placed into a greenhouse.Emerging seedlings were counted and identified.Effect of successional stage and origin of samples on mean and variability of abundance and species richness of seedlings emerging from casts and soil seed banks were analyzed.Plant compositions were compared between all sample types.We used generalized mixed-effect models and a distance-based redundancy multivariate analysis.Important Findings Seedling abundance was always higher in earthworm casts than in the soil seed bank and increased up to 5-fold,4-fold and 3.5-fold,respectively,in the tall grassland,woods and encroached grassland compared to the soil surface layer.Species richness was also higher in earthworm casts than in the soil seed bank in all successional stages,with a 4-fold increase in the encroached grassland.The plant composition of the standing vegetation was more similar to that of seedlings from casts than to that of seedlings from the soil seed bank.Seedlings diversity emerging from casts in the tall and encroached grasslands tended toward the diversity found in woods.Our results indicate that earthworms may promote the emergence of seedlings.We also suggest that the loss of some plant species in the seed bank and the tall grass vegetation in intermediary successional stages modify the local conditions and prevent the further establishment of early-successional plant species.

Spatial patterns of a savanna palm tree Borassus aethiopum and its temporal variability

The spatial distributions and associations of plant species in a stand can provide essential information about their dynamics.However,since tree spatial distribution and association depend on factors that operate at different scales,disentangling the effects of environmental heterogeneity and plant-plant interactions requires the choice of a suitable null model for spatial analysis.We analyzed the spatial distributions of the same savanna palm tree populations that were studied,the underlying hypothesis being that these distributions have changed in 20 years.To remove the effect of large-scale environmental heterogeneity,the inhomogeneous L-function under the heterogeneous Poisson null model was used.We showed:(i)unlike 20 years ago,adults had a regular distribution instead of an aggregated distribution in the grass savanna;(i)although the spatial distribution of seedlings and juveniles was always aggregated,we observed a decrease in the size of the aggregates(intensities);ili)except for juveniles,no other stages were associated with nutrient-rich patches,which was also different 20 years ago;(iv)we did not observe any particular difference in structure between two study sites,only that female palm trees were spatially associated with entire-leafed seedlings in the reserve while they were spatially independent in the rural area.Our study supports the hypothesis that the spatial distributions have partially changed,and that the management of spatial heterogeneity has improved and given more precision in the results.