Aim: To evaluate the efficacy of topical aldose reductase inhibitor CT-112 (5 -[3-ethoxy-4-pentyloxyphenyl]2,4-thiazolidinedione) on corneal epitheli al barrier function in diabetic patients. Methods: This was a prosp...Aim: To evaluate the efficacy of topical aldose reductase inhibitor CT-112 (5 -[3-ethoxy-4-pentyloxyphenyl]2,4-thiazolidinedione) on corneal epitheli al barrier function in diabetic patients. Methods: This was a prospective, randomi sed, double masked placebo controlled study. 34 eyes of 34 diabetic patients wer e randomly assigned treatment with 0.25%eye drops of CT-112 (n=22) or a placeb o (n=12) four times a day for 8 weeks. Corneal fluorescein staining and corneal sensation were examined before treatment as well as 4 and 8 weeks after administ ration. Corneal epithelial permeability to fluorescence was measured with an ant erior fluorophotometer. Results: Average scores of superficial punctate keratopa thy and corneal sensitivity did not differ significantly between the two groups at any time. Whereas average fluorescein concentrations did not differ significa ntly for the CT-112 and placebo groups before treatment, they did differ signif icantly 4 and 8 weeks after treatment (4 weeks, p=0.0327; 8 weeks, p=0.0143). Co nclusion: The topical aldose reductase inhibitor, CT-112 improves the corneal e pithelial barrier function in diabetic patients.展开更多
Background: Fibulin- 5 was recently found as a secreted extracellular matrix protein that functions as a scaffold for elastic fibres. However, the distribution of fibulin- 5 in human skin and its changes during the ag...Background: Fibulin- 5 was recently found as a secreted extracellular matrix protein that functions as a scaffold for elastic fibres. However, the distribution of fibulin- 5 in human skin and its changes during the ageing process are not known. Objectives: To explore the involvement of fibulin- 5 in skin ageing, the age- dependent changes in fibulin- 5 localization in human skin were examined compared with those of other elastic fibre components including elastin, fibrillin- 1 and fibulin- 2. Methods: The distribution of elastin, fibrillin- 1, fibrillin- 2, fibulin- 2 and fibulin- 5 was investigated by means of immunohistochemistry using their specific antibodies. Skin samples were recovered from 12 healthy subjects undergoing plastic surgery. Ultraviolet (UV) B- irradiated or control nonirradiated buttock skin samples were obtained from two healthy volunteers at 2 days after the irradiation at 2 minimal erythemal doses. Results: In the reticular dermis of young sun- protected skin from the upper arm, fibulin- 5 colocalized with the other elastic fibre components, while in the papillary dermis fibulin- 5 showed candelabra- like structures perpendicular to the epidermiswith an unstained area just beneath the epidermis, which was similar to that of elastin but not fibrillin- 1. Fibulin- 5 in the reticular dermis decreased and disappeared with age even in sun- protected skin from the thigh, abdomen and upper arm. In sun- exposed skin, fibulin- 5 was extremely reduced in the dermis of cheek skin even from a 20- year- old man. UVB irradiation reduced fibulin- 5, fibulin- 2 and elastin markedly, moderately and weakly, respectively, compared with levels in control nontreated skin. Interestingly, the deposition of fibulin- 5 was increased in solar elastosis, like that of other elastic fibre components. Conclusions: These results suggest that fibulin- 5 is a good marker of skin ageing and that the earlier loss of fibulin- 5 may involve age- dependent changes in other elastic fibre components.展开更多
文摘Aim: To evaluate the efficacy of topical aldose reductase inhibitor CT-112 (5 -[3-ethoxy-4-pentyloxyphenyl]2,4-thiazolidinedione) on corneal epitheli al barrier function in diabetic patients. Methods: This was a prospective, randomi sed, double masked placebo controlled study. 34 eyes of 34 diabetic patients wer e randomly assigned treatment with 0.25%eye drops of CT-112 (n=22) or a placeb o (n=12) four times a day for 8 weeks. Corneal fluorescein staining and corneal sensation were examined before treatment as well as 4 and 8 weeks after administ ration. Corneal epithelial permeability to fluorescence was measured with an ant erior fluorophotometer. Results: Average scores of superficial punctate keratopa thy and corneal sensitivity did not differ significantly between the two groups at any time. Whereas average fluorescein concentrations did not differ significa ntly for the CT-112 and placebo groups before treatment, they did differ signif icantly 4 and 8 weeks after treatment (4 weeks, p=0.0327; 8 weeks, p=0.0143). Co nclusion: The topical aldose reductase inhibitor, CT-112 improves the corneal e pithelial barrier function in diabetic patients.
文摘Background: Fibulin- 5 was recently found as a secreted extracellular matrix protein that functions as a scaffold for elastic fibres. However, the distribution of fibulin- 5 in human skin and its changes during the ageing process are not known. Objectives: To explore the involvement of fibulin- 5 in skin ageing, the age- dependent changes in fibulin- 5 localization in human skin were examined compared with those of other elastic fibre components including elastin, fibrillin- 1 and fibulin- 2. Methods: The distribution of elastin, fibrillin- 1, fibrillin- 2, fibulin- 2 and fibulin- 5 was investigated by means of immunohistochemistry using their specific antibodies. Skin samples were recovered from 12 healthy subjects undergoing plastic surgery. Ultraviolet (UV) B- irradiated or control nonirradiated buttock skin samples were obtained from two healthy volunteers at 2 days after the irradiation at 2 minimal erythemal doses. Results: In the reticular dermis of young sun- protected skin from the upper arm, fibulin- 5 colocalized with the other elastic fibre components, while in the papillary dermis fibulin- 5 showed candelabra- like structures perpendicular to the epidermiswith an unstained area just beneath the epidermis, which was similar to that of elastin but not fibrillin- 1. Fibulin- 5 in the reticular dermis decreased and disappeared with age even in sun- protected skin from the thigh, abdomen and upper arm. In sun- exposed skin, fibulin- 5 was extremely reduced in the dermis of cheek skin even from a 20- year- old man. UVB irradiation reduced fibulin- 5, fibulin- 2 and elastin markedly, moderately and weakly, respectively, compared with levels in control nontreated skin. Interestingly, the deposition of fibulin- 5 was increased in solar elastosis, like that of other elastic fibre components. Conclusions: These results suggest that fibulin- 5 is a good marker of skin ageing and that the earlier loss of fibulin- 5 may involve age- dependent changes in other elastic fibre components.
