Background:The increasing occurrence of diabetes mellitus(DM)noted worldwide has considerably elicited concern in the recent past.DM is associated with elevated vascular complications,morbidity,mortality,and poor qual...Background:The increasing occurrence of diabetes mellitus(DM)noted worldwide has considerably elicited concern in the recent past.DM is associated with elevated vascular complications,morbidity,mortality,and poor quality of life.In this context,mesenchymal stem cells(MSCs)have shown significant therapeutic potentialities in managing and curing type 1 DM owing to their self-renewable,immunosuppressive,and differentiation capacities.We investigated the potential action of N,N′-diphenyl-1,4-phenylenediamine(DPPD),a well-known synthetic antioxidant to enhance the therapeutic ability of the adipose-derived stem cells(AD-MSCs)in alleviating kidney and liver complications in diabetic rats.Methods:Over the four weeks of experiments,albino male rats(n=36)were split into six test groups:control,DPPD(250 mg/kg,i.p.),STZ-diabetic(D),D+DPPD,D+AD-MSCs(1×10^(6)cell/rat,i.v.),and D+AD-MSCs+DPPD treated groups.Results:Significant declines in the renal and hepatic oxidative stress markers(MDA,ROS,and AGEs)were observed coupled with a significant elevation in many antioxidant marker levels(GSH,SOD,CAT,GPx,HO-1,and TAC)in the diabetic rats treated with either DPPD or AD-MSCs or their co-administered injection compared to the diabetic untreated rats.This was suggested to be the leading cause of amelioration of the kidney functions(as measured by urea,uric acid,and creatine levels)and liver functions(as evidenced by the levels of AST,ALT,ALP,bilirubin,total proteins,albumin,and globulins).Conclusion:DPPD and AD-MSCs co-administration showed superior results in terms of the enhancement of the relative hepato-renal function,indicating the beneficial role of DPPD supplementation in increasing the therapeutic potential of AD-MSCs.展开更多
The development of a new silane reagent derived from tetrachlorosilane (TCS) was applied in the present work. TCS-sodium nitrate (NaNO3) binary reagent and zinc chloride (ZnCl2) were reported here as a homogeneous nit...The development of a new silane reagent derived from tetrachlorosilane (TCS) was applied in the present work. TCS-sodium nitrate (NaNO3) binary reagent and zinc chloride (ZnCl2) were reported here as a homogeneous nitrating system. The later was used for the efficient mono nitration, in most cases, with high para-regioselectivity. The nitration proceeded smoothly under mild condition, fairly clean and in good yields. This readily available and inexpensive system is superior to other methods by avoidance of the use of corrosive nitrating reagents and therefore considered to be convenient in terms of risk reduction, economic advantages and environment protection. The present protocol was convenient and applicable to a variety of aromatic hydrocarbons and could be amenable to high throughput synthesis of combinatorial libraries for potential drug development, which needs to be studied as part of future investigations.展开更多
基金the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research at King Faisal University,Saudi Arabia,for financial support under the annual funding track(Grant 3730).
文摘Background:The increasing occurrence of diabetes mellitus(DM)noted worldwide has considerably elicited concern in the recent past.DM is associated with elevated vascular complications,morbidity,mortality,and poor quality of life.In this context,mesenchymal stem cells(MSCs)have shown significant therapeutic potentialities in managing and curing type 1 DM owing to their self-renewable,immunosuppressive,and differentiation capacities.We investigated the potential action of N,N′-diphenyl-1,4-phenylenediamine(DPPD),a well-known synthetic antioxidant to enhance the therapeutic ability of the adipose-derived stem cells(AD-MSCs)in alleviating kidney and liver complications in diabetic rats.Methods:Over the four weeks of experiments,albino male rats(n=36)were split into six test groups:control,DPPD(250 mg/kg,i.p.),STZ-diabetic(D),D+DPPD,D+AD-MSCs(1×10^(6)cell/rat,i.v.),and D+AD-MSCs+DPPD treated groups.Results:Significant declines in the renal and hepatic oxidative stress markers(MDA,ROS,and AGEs)were observed coupled with a significant elevation in many antioxidant marker levels(GSH,SOD,CAT,GPx,HO-1,and TAC)in the diabetic rats treated with either DPPD or AD-MSCs or their co-administered injection compared to the diabetic untreated rats.This was suggested to be the leading cause of amelioration of the kidney functions(as measured by urea,uric acid,and creatine levels)and liver functions(as evidenced by the levels of AST,ALT,ALP,bilirubin,total proteins,albumin,and globulins).Conclusion:DPPD and AD-MSCs co-administration showed superior results in terms of the enhancement of the relative hepato-renal function,indicating the beneficial role of DPPD supplementation in increasing the therapeutic potential of AD-MSCs.
文摘The development of a new silane reagent derived from tetrachlorosilane (TCS) was applied in the present work. TCS-sodium nitrate (NaNO3) binary reagent and zinc chloride (ZnCl2) were reported here as a homogeneous nitrating system. The later was used for the efficient mono nitration, in most cases, with high para-regioselectivity. The nitration proceeded smoothly under mild condition, fairly clean and in good yields. This readily available and inexpensive system is superior to other methods by avoidance of the use of corrosive nitrating reagents and therefore considered to be convenient in terms of risk reduction, economic advantages and environment protection. The present protocol was convenient and applicable to a variety of aromatic hydrocarbons and could be amenable to high throughput synthesis of combinatorial libraries for potential drug development, which needs to be studied as part of future investigations.
