Relationship between antioxidant capacity and oxidative stress in children with acute hepatitis A

AIM： To investigate in children with acute hepatitis A. According to our knowledge, there are no data about the blood levels of malondialdehyde （MDA, an indicator of oxidative stress） and nonenzymic antioxidants in children with acute hepatitis A. METHODS： Whole blood MDA and reduced glutathione （GSH）, serum β-carotene, retinol, vitamin E and vitamin C levels were studied in 19 （10 females, 9 males） children with acute hepatitis A and in 29 （β females, 16 males） healthy control subjects. RESULTS： There was a statistically significant difference between patients and controls for all parameters （P 〈 0.05）. Lipid peroxidation marker MDA was significantly elevated （P 〈 0.001）, while antioxidants β-carotene, retinol and GSH were significantly decreased （all P 〈 0.001） in patients compared to healthy subjects. In addition, o-tocopherol and ascorbic acid levels were significantly lower in patients when compared to age and sex matched controls （P 〈 0.05, P 〈 0.01, respectively）. CONCLUSION： Our study shows that hepatitis A virus induces oxidative stress in childern with hepatitis A. This finding could be taken into consideration to improve the therapeutic approach in acute hepatitis A.

DNA damage-induced by sodium flouride (NaF) and the effect ofcholicalciferol

It is known that the high electronegativity of fluorine affects various soft tissues,especially the bone structure in organisms.Of these tissues are the kidneys,which play an important role in the excretion of fluoride from the body.Fluoride affects many cellular mechanisms.One of these effects is DNA damage.Our study aimed to investigate the likely protective effect of cholecalciferol(vitamin D3)on genomic DNA damage-induced NaF depending on concentration and time.The IC25 and IC50 values of NaF for 3,12 and 24 h and optimum dose of increase in proliferation to vitamin D3 through MTT assay in NRK-52E kidney cells were determined.DNA damage was significantly increased(p<0.05)compared to the control group in all groups except for vitamin D3.It was determined that treatment with NaF together with vitamin D3 decreased the DNA damage compared to NaF treated groups for 3 and 12 h.NaF combined with vitamin D3 was determined statistically to decrease(p<0.05)DNA damage compared to NaF treated groups for 24 h.As a result,it was determined that the treatment with cytotoxic concentration NaF depending on the time significantly increased(p<0.05)the genomic DNA damage,but NaF treatment together with vitamin D3 decreased the DNA damage in renal cells depending on the time.It was concluded that vitamin D3 may be useful in preventing DNA damage caused by NaF.