Objective This study aimed to clarify the intervention effect of salidroside(SAL)on lung injury caused by PM_(2.5) in mice and illuminate the function of SIRT1-PGC-1ɑaxis.Methods Specific pathogen-free(SPF)grade male...Objective This study aimed to clarify the intervention effect of salidroside(SAL)on lung injury caused by PM_(2.5) in mice and illuminate the function of SIRT1-PGC-1ɑaxis.Methods Specific pathogen-free(SPF)grade male C57BL/6 mice were randomly assigned to the following groups:control group,SAL group,PM_(2.5) group,SAL+PM_(2.5) group.On the first day,SAL was given by gavage,and on the second day,PM_(2.5) suspension was given by intratracheal instillation.The whole experiment consist of a total of 10 cycles,lasting 20 days.At the end of treatment,blood samples and lung tissues were collected and analyzed.Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy.The expression of inflammatory,antioxidants,apoptosis,and SIRT1-PGC-1ɑproteins were detected by Western blotting.Results Exposure to PM_(2.5) leads to obvious morphological and pathologica changes in the lung of mice.PM_(2.5) caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1,Nrf2,SOD2,SIRT1 and PGC-1ɑ,and an increase in the protein expressions of IL-6,IL-1β,Bax,caspase-9 and cleaved caspase-3.However,SAL reversed the aforementioned changes caused by PM_(2.5) by activating the SIRT1-PGC-1α pathway.Conclusion SAL can activate SIRT1-PGC-1ɑ to ameliorate PM2.5-induced lung injury.展开更多
目的系统评价持续被动运动锻炼对全膝关节置换术后患者下肢深静脉血栓、膝关节功能的作用。方法检索CNKI、万方、VIP、Proquest、CIHNAL、JBI、cochrane library、Web of science、PubMed、Embase等数据库,查找所有符合纳入和排除标准...目的系统评价持续被动运动锻炼对全膝关节置换术后患者下肢深静脉血栓、膝关节功能的作用。方法检索CNKI、万方、VIP、Proquest、CIHNAL、JBI、cochrane library、Web of science、PubMed、Embase等数据库,查找所有符合纳入和排除标准的中英文文献,进行文献质量双人评价,纳入的文献利用RevMan 5.3进行Meta分析。结果纳入16项随机对照试验,Meta分析结果表明:与常规主动运动相比,膝关节置换术后患者运用持续被动运动锻炼可以提高早期膝关节活动度[WMD=4.12,95%CI(0.98,7.25),P=0.01],但在预防膝关节置换术后下肢静脉血栓[OR=0.64,95%CI(0.18,2.32),P=0.50]、膝关节功能[WMD=0.09,95%CI(-0.05,0.23),P=0.23]、术后疼痛[WMD=0.05,95%CI(-0.11,0.22),P=0.54]、住院时间[WMD=0.33,95%CI(-0.2,0.85),P=0.23]等方面无明显作用。结论膝关节置换术后患者持续被动运动锻炼可以改善膝关节早期活动度,但尚无足够证据表明持续被动运动锻炼可以降低下肢静脉血栓、改善膝关节功能、改善术后疼痛、缩短住院时间。展开更多
基金supported by Shandong Provincial Natural Science Foundation,China(No.ZR2020MH336)Weifang Science and Technology Development Plan Project(NO.2022GX015,NO.2022GX010).
文摘Objective This study aimed to clarify the intervention effect of salidroside(SAL)on lung injury caused by PM_(2.5) in mice and illuminate the function of SIRT1-PGC-1ɑaxis.Methods Specific pathogen-free(SPF)grade male C57BL/6 mice were randomly assigned to the following groups:control group,SAL group,PM_(2.5) group,SAL+PM_(2.5) group.On the first day,SAL was given by gavage,and on the second day,PM_(2.5) suspension was given by intratracheal instillation.The whole experiment consist of a total of 10 cycles,lasting 20 days.At the end of treatment,blood samples and lung tissues were collected and analyzed.Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy.The expression of inflammatory,antioxidants,apoptosis,and SIRT1-PGC-1ɑproteins were detected by Western blotting.Results Exposure to PM_(2.5) leads to obvious morphological and pathologica changes in the lung of mice.PM_(2.5) caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1,Nrf2,SOD2,SIRT1 and PGC-1ɑ,and an increase in the protein expressions of IL-6,IL-1β,Bax,caspase-9 and cleaved caspase-3.However,SAL reversed the aforementioned changes caused by PM_(2.5) by activating the SIRT1-PGC-1α pathway.Conclusion SAL can activate SIRT1-PGC-1ɑ to ameliorate PM2.5-induced lung injury.
文摘目的系统评价持续被动运动锻炼对全膝关节置换术后患者下肢深静脉血栓、膝关节功能的作用。方法检索CNKI、万方、VIP、Proquest、CIHNAL、JBI、cochrane library、Web of science、PubMed、Embase等数据库,查找所有符合纳入和排除标准的中英文文献,进行文献质量双人评价,纳入的文献利用RevMan 5.3进行Meta分析。结果纳入16项随机对照试验,Meta分析结果表明:与常规主动运动相比,膝关节置换术后患者运用持续被动运动锻炼可以提高早期膝关节活动度[WMD=4.12,95%CI(0.98,7.25),P=0.01],但在预防膝关节置换术后下肢静脉血栓[OR=0.64,95%CI(0.18,2.32),P=0.50]、膝关节功能[WMD=0.09,95%CI(-0.05,0.23),P=0.23]、术后疼痛[WMD=0.05,95%CI(-0.11,0.22),P=0.54]、住院时间[WMD=0.33,95%CI(-0.2,0.85),P=0.23]等方面无明显作用。结论膝关节置换术后患者持续被动运动锻炼可以改善膝关节早期活动度,但尚无足够证据表明持续被动运动锻炼可以降低下肢静脉血栓、改善膝关节功能、改善术后疼痛、缩短住院时间。
