Nervosine Ⅶ is one of the known saturated pyrrolizidine alkaloids isolated from the plant of Liparis nervosa.This is first study to investigate the antitumor activity of nervosine Ⅶ in vitro,and the results indicate...Nervosine Ⅶ is one of the known saturated pyrrolizidine alkaloids isolated from the plant of Liparis nervosa.This is first study to investigate the antitumor activity of nervosine Ⅶ in vitro,and the results indicated that nervosine Ⅶ induced autophagy and apoptosis in HCT116 human colorectal cancer cells.Mechanistic studies showed that nervosine Ⅶ-induced apoptosis was associated with the intrinsic pathway by the activation of caspase-9,-3 and-7.Autophagy induced by nervosine Ⅶ was characteristic with the regulation of autophagic markers including the increase of LC3-Ⅱ and beclin 1 proteins,and the decrease of p62 protein.Nervosine Ⅶ simultaneously induced autophagy and apoptosis by activated MAPKs signaling pathway including JNK,ERK1/2 and p38,suppressing the p53 signaling pathway.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.81402803).
文摘Nervosine Ⅶ is one of the known saturated pyrrolizidine alkaloids isolated from the plant of Liparis nervosa.This is first study to investigate the antitumor activity of nervosine Ⅶ in vitro,and the results indicated that nervosine Ⅶ induced autophagy and apoptosis in HCT116 human colorectal cancer cells.Mechanistic studies showed that nervosine Ⅶ-induced apoptosis was associated with the intrinsic pathway by the activation of caspase-9,-3 and-7.Autophagy induced by nervosine Ⅶ was characteristic with the regulation of autophagic markers including the increase of LC3-Ⅱ and beclin 1 proteins,and the decrease of p62 protein.Nervosine Ⅶ simultaneously induced autophagy and apoptosis by activated MAPKs signaling pathway including JNK,ERK1/2 and p38,suppressing the p53 signaling pathway.
