Spraying Arginine at 5 Days before Harvest Delays Postharvest Broccoli Senescence via Nutrient Accumulation

To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before harvest.The appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of storage.The results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli color.Furthermore,during the growth stage,total sugar accumulation was higher in the 5-ARG group.In addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest broccoli.In conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of broccoli.Therefore,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.

基于植物代谢组学的不同基原淫羊藿差异性研究

目的 研究不同基原和产地淫羊藿的化学成分差异性。方法 采用液相色谱-串联质谱(LC-MS/MS)联用技术对淫羊藿进行代谢组学分析,筛选得到差异成分。同时采用液相色谱法、紫外分光光度法分别测定总黄酮醇苷、总黄酮含量,研究不同基原淫羊藿中指标成分的含量差异。结果 总黄酮醇苷和总黄酮含量在不同基原淫羊藿中存在一定差异。不同区域淫羊藿整体化学成分具有差异性,且产地和基原表现出对应性。心叶淫羊藿与朝鲜淫羊藿间存在65个差异成分,与柔毛淫羊藿之间存在125个差异成分,与箭叶淫羊藿之间存在109个差异成分,其中共有差异成分19个。结论 本研究揭示了不同基原淫羊藿化学成分的差异性,为淫羊藿的基原鉴别提供了参考,有利于淫羊藿资源的合理利用。