The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.Howeve...The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.However,our understanding of the TRPV4 in fish remains poor.Therefore,we studied the TRPV4 gene from Cynoglossus semilaevis,named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4,including the domains of ANK repeats,six TM,TRP domain,and CaMBD.The CsTRPV4 was expressed ubiquitously in examined tissues:higher expression in the heart,spleen,testis,and eye,but lower expression in kidney and liver.Surprisingly,the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free neuromasts.In addition,CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts.Therefore,the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in C.semilaevis,which lays a foundation for further study of the functions of the free neuromasts.展开更多
A full-length lily-type lectin （SmLTL） was identified from turbot （Scophthalmus maximus） in this study. By searching database for protein identification and fimction prediction, SmLTL were confirmed. The full-leng...A full-length lily-type lectin （SmLTL） was identified from turbot （Scophthalmus maximus） in this study. By searching database for protein identification and fimction prediction, SmLTL were confirmed. The full-length cDNA of SmLTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The SmLTL peptide is characterized by a specific J3-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30-99; two of the repeats share the classical mannose binding domain （QxDxNxVxY） while the third binding site was similar to other fish-specific binding motifs （TxTxGxRxV）. The primary, secondary, and tertiary structures of SmLTL were predicted and analyzed, indicating that the SmLTL protein was hydrophilic, contained 5.36% a-helices, 39.29% extended strands, 16.07% [3-folds, and 39.29% random coils, and three [3-folds. Quantitative real- time polymerase chain reaction （qPCR） analysis revealed that the SmLTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of SmLTL expression were observed in other tissues. The expression of SmLTL in gill, skin and intestine increased at mRNA level after stimulation of Hbrio anguillarum, our results suggest that SmLTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that SmLTL is a member of the lily- type lectin family and the information reported here will provide an important foundation for future research on the role of this protein.展开更多
基金Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-47-G01)the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-OS04)the Qingdao Natural Science Foundation(No.12-1-4-12-(1)-jch)
文摘The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.However,our understanding of the TRPV4 in fish remains poor.Therefore,we studied the TRPV4 gene from Cynoglossus semilaevis,named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4,including the domains of ANK repeats,six TM,TRP domain,and CaMBD.The CsTRPV4 was expressed ubiquitously in examined tissues:higher expression in the heart,spleen,testis,and eye,but lower expression in kidney and liver.Surprisingly,the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free neuromasts.In addition,CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts.Therefore,the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in C.semilaevis,which lays a foundation for further study of the functions of the free neuromasts.
基金Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-50-G01)the General Financial Grant from the China Postdoctoral Science Foundation(No.2015-2016)+4 种基金the Special Financial Grant from the China Postdoctoral Science Foundation(No.2016T90661)the Shandong Provincial Natural Science Foundation(No.ZR2014CP001)the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A408-8)the Primary Research&Developement Plan of Shandong Province(No.2016GSF115019)the Shandong Agriculture Seed Project(No.2016LZGC031)
文摘A full-length lily-type lectin （SmLTL） was identified from turbot （Scophthalmus maximus） in this study. By searching database for protein identification and fimction prediction, SmLTL were confirmed. The full-length cDNA of SmLTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The SmLTL peptide is characterized by a specific J3-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30-99; two of the repeats share the classical mannose binding domain （QxDxNxVxY） while the third binding site was similar to other fish-specific binding motifs （TxTxGxRxV）. The primary, secondary, and tertiary structures of SmLTL were predicted and analyzed, indicating that the SmLTL protein was hydrophilic, contained 5.36% a-helices, 39.29% extended strands, 16.07% [3-folds, and 39.29% random coils, and three [3-folds. Quantitative real- time polymerase chain reaction （qPCR） analysis revealed that the SmLTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of SmLTL expression were observed in other tissues. The expression of SmLTL in gill, skin and intestine increased at mRNA level after stimulation of Hbrio anguillarum, our results suggest that SmLTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that SmLTL is a member of the lily- type lectin family and the information reported here will provide an important foundation for future research on the role of this protein.
