Squaliobarbus curriculus is a eurythermic ?sh found mainly in East Asia. The natural germplasm resources of this species, an organism of potential economic aquacultural importance, have recently been threatened by ove...Squaliobarbus curriculus is a eurythermic ?sh found mainly in East Asia. The natural germplasm resources of this species, an organism of potential economic aquacultural importance, have recently been threatened by over?shing and habitat degradation. The objective of this study was to obtain functional genomic resources and candidate microsatellite markers for future genetic observation and germplasm protection of S. curriculus. Illumina paired-end sequencing generated over 22.20 million clean reads, which were assembled into 204 027 transcripts with an average length of 1 457 bp. A total of 112 570 protein-coding sequences(55.17%) were predicted, 111 934 of which had signi?cant matches in the NCBI nonredundant protein database. Overall, 60 450, 34 830, and 77 755 transcripts were assigned to Gene Ontology, Clusters of Orthologous Groups, and Eukaryotic Orthologous Groups categories, respectively.In addition, 44 781 transcripts(21.95%) were mapped to 230 KEGG pathways. A total of 51 426 candidate simple sequence repeat(SSR) markers were identi?ed and used to design 36 149 PCR primer pairs. Fifty of these primer pairs were randomly selected for PCR validation in 32 S. curriculus specimens, which resulted in 25 pairs yielding the expected products. Further analysis revealed that 40%(10) of the 25 ampli?ed loci were polymorphic. The transcriptomic pro?le and SSR repertoire obtained in this study should facilitate population genetic studies and germplasm resource protection of S. curriculus.展开更多
针对现有两层无线传感器网络隐私保护范围查询协议存在数据安全低、感知节点通信能耗较高,且较少针对多维数据的问题,提出了一种基于交叉0-1编码和质数融合的两层无线传感器网络隐私保护范围查询协议。在数据提交阶段,感知节点采集多维...针对现有两层无线传感器网络隐私保护范围查询协议存在数据安全低、感知节点通信能耗较高,且较少针对多维数据的问题,提出了一种基于交叉0-1编码和质数融合的两层无线传感器网络隐私保护范围查询协议。在数据提交阶段,感知节点采集多维数据并根据属性维度分组,采用交叉0-1编码、质数融合等方法优化比较因子的计算方式,用AES算法构建加密约束链,提高数据安全性,降低计算和通信能耗。在查询处理阶段,Sink节点对查询范围值采用交叉0-1编码和质数融合操作产生比较因子,将查询单元格与比较因子作为查询指令送至存储节点;存储节点根据交叉0-1编码比较规则将采集数据与查询范围值的比较因子比较,完成多维数据范围查询,结果发送给Sink节点。在结果验证阶段,Sink节点根据多维加密约束链中的采集周期时间和特性,验证查询结果的真实性完整性。在实验部分,采用Cortex-M4和Cortex-A9内核开发板实现协议内容,验证了数据提交、隐私数据查询、隐私数据查询结果真实性和完整性验证等功能。通过对本文协议与CSRQ(communication-efficient secure range queries)协议在感知节点通信能耗数据的实验结果对比,表明在同等实验环境下本文协议的通信能耗比CSRQ协议低20%左右。展开更多
基金Supported by the Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection,Huaiyin Normal University(Germplasm Production and Culture of S.curriculus,No.HSXT219)the Scientific Fund of Jiangsu Province(No.BY2015039-10)the Priority Academic Development Program of Jiangsu Higher Education Institutions,China
文摘Squaliobarbus curriculus is a eurythermic ?sh found mainly in East Asia. The natural germplasm resources of this species, an organism of potential economic aquacultural importance, have recently been threatened by over?shing and habitat degradation. The objective of this study was to obtain functional genomic resources and candidate microsatellite markers for future genetic observation and germplasm protection of S. curriculus. Illumina paired-end sequencing generated over 22.20 million clean reads, which were assembled into 204 027 transcripts with an average length of 1 457 bp. A total of 112 570 protein-coding sequences(55.17%) were predicted, 111 934 of which had signi?cant matches in the NCBI nonredundant protein database. Overall, 60 450, 34 830, and 77 755 transcripts were assigned to Gene Ontology, Clusters of Orthologous Groups, and Eukaryotic Orthologous Groups categories, respectively.In addition, 44 781 transcripts(21.95%) were mapped to 230 KEGG pathways. A total of 51 426 candidate simple sequence repeat(SSR) markers were identi?ed and used to design 36 149 PCR primer pairs. Fifty of these primer pairs were randomly selected for PCR validation in 32 S. curriculus specimens, which resulted in 25 pairs yielding the expected products. Further analysis revealed that 40%(10) of the 25 ampli?ed loci were polymorphic. The transcriptomic pro?le and SSR repertoire obtained in this study should facilitate population genetic studies and germplasm resource protection of S. curriculus.
文摘针对现有两层无线传感器网络隐私保护范围查询协议存在数据安全低、感知节点通信能耗较高,且较少针对多维数据的问题,提出了一种基于交叉0-1编码和质数融合的两层无线传感器网络隐私保护范围查询协议。在数据提交阶段,感知节点采集多维数据并根据属性维度分组,采用交叉0-1编码、质数融合等方法优化比较因子的计算方式,用AES算法构建加密约束链,提高数据安全性,降低计算和通信能耗。在查询处理阶段,Sink节点对查询范围值采用交叉0-1编码和质数融合操作产生比较因子,将查询单元格与比较因子作为查询指令送至存储节点;存储节点根据交叉0-1编码比较规则将采集数据与查询范围值的比较因子比较,完成多维数据范围查询,结果发送给Sink节点。在结果验证阶段,Sink节点根据多维加密约束链中的采集周期时间和特性,验证查询结果的真实性完整性。在实验部分,采用Cortex-M4和Cortex-A9内核开发板实现协议内容,验证了数据提交、隐私数据查询、隐私数据查询结果真实性和完整性验证等功能。通过对本文协议与CSRQ(communication-efficient secure range queries)协议在感知节点通信能耗数据的实验结果对比,表明在同等实验环境下本文协议的通信能耗比CSRQ协议低20%左右。