期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
3种气单胞菌鉴定方法的比较
1
作者 杨致旭 王楠楠 +3 位作者 沈广龙 吴亚锋 方光远 郭宇飞 《水产养殖》 CAS 2023年第12期14-19,36,共7页
采用分子生物学检测方法、API 20NE生化鉴定法以及梅里埃(VITEK MS)全自动快速微生物质谱检测法,对60株试验菌株进行种属鉴定,比较3种鉴定方法的准确性和优缺点。对试验菌株进行16S r DNA特异性引物扩增和测序,确定菌株种属,并作为比较... 采用分子生物学检测方法、API 20NE生化鉴定法以及梅里埃(VITEK MS)全自动快速微生物质谱检测法,对60株试验菌株进行种属鉴定,比较3种鉴定方法的准确性和优缺点。对试验菌株进行16S r DNA特异性引物扩增和测序,确定菌株种属,并作为比较3种鉴定方法的标准。随后用API 20NE生化鉴定法和梅里埃(VITEK MS)全自动快速微生物质谱检测法,对试验菌株进行鉴定。结果显示,分子生物学方法和生化鉴定法,对60株菌的检出率均为100%,而质谱仪的检出率仅为60.0%。使用分子生物学法共检出44株气单胞菌(100%),准确率为100%;使用生化鉴定法对气单胞菌的检出率为88.6%,准确率为59.1%;使用质谱仪对气单胞菌的检出率为54.5%,准确率为38.6%。3种鉴定方法对气单胞菌的检出率和准确率从大到小为:分子生物学法、API 20NE生化鉴定、质谱仪。 展开更多
关键词 细菌鉴定 分子生物学法 API 20NE生化鉴定 微生物质谱检测
下载PDF
A Carbon Isotopic Stratigraphic Pattern of the Late Palaeozoic Coals in the North China Platform and Its Palaeoclimatic Implications 被引量:7
2
作者 ZHANG Hong shen guanglong HE Zonglian 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 1999年第1期111-119,共9页
This paper gives the stable carbon isotopic data in coals from the Late Namurian to Kazanian stages in the Serteng Mt., Xishan and Huainan coalfields of the North China Platform. Its stratigraphic pattern shows that s... This paper gives the stable carbon isotopic data in coals from the Late Namurian to Kazanian stages in the Serteng Mt., Xishan and Huainan coalfields of the North China Platform. Its stratigraphic pattern shows that several isotopic shifts are apparent, and the large δ13C negative shifts (approximately 2.5 to 3.0 %%) occurred during the Stephanian, Artinskian and Kazanian are observed in three Permo-Carboniferous coalfields. Those negative shifts are neither related to the coal rank and coal macerals, nor caused by the variety of peat-forming plants. The general decrease in the δ13C values of the Stephanian, Artinskian and Kazanian coals is consistent with an overall decrease in the δ13C values of ambient atmospheric CO2 and/or a relative increase in atmospheric Pco2 during the coal-forming periods. Therefore the authors postulate that the oxidation of peat, and the δ13C-depleted CO2 flux into the atmosphere during the above stages may have contributed to coeval palaeoclimatic warming by way of the greenhouse effect. 展开更多
关键词 carbon isotope COAL PALAEOCLIMATE Late Palaeozoic the North China Platform
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部