The Strait of Malacca （SoM）, the world＇s busiest sea-route, is increasingly polluted as the rapid develop- ment of world trades, affecting phytoplankton primary productivity therein. The variations of surface phy- ...The Strait of Malacca （SoM）, the world＇s busiest sea-route, is increasingly polluted as the rapid develop- ment of world trades, affecting phytoplankton primary productivity therein. The variations of surface phy- toplankton biomass, size-structure and carbon fixation were investigated across the SoM during the spring period （May 4 to 9, 2011）. Chlorophyll a concentration increased from 0.12 ptg/L at the northwest entrance of the SoM to a maximal 0.63 #g/L at narrowest section, and decreased to 0.10/.tg/L at the southeast entrance. Photosynthetic carbon fixation by phytoplankton coincided well with Chl a biomass, and increased from 10.8 to 22.3 pg C/（L.d）, then decreased to 9.21/zg C/（L.d）; while the carbon fixation rate showed an inverse pattern to the changes of Chl a, and decreased from 87.1 to 35.5 #g C/（#g Chl a.d） and increased thereafter to 95.3 btg C/（/2g Chl a.d）. Picophytoplankton cells （〈3/2m） contributed to more than 60% and 50% of the total Chl a and carbon fixation at both the entry waters; while the contributions of pico-cells decreased sharply to the minimum of 18.3% and 27.5% at the narrowest part of the SoM. In particular, our results showed that the silicate concentration positively regulated Chl a biomass and carbon fixation, reflecting that the higher silicate favoured the growth of phytoplankton and thus led to higher primary production in this strait.展开更多
Ciliates are very important components in most marine ecosystem. They are trophic link between the microbial food web and grazing food chain. In this study, ciliates were collected from 11 sites in the southern South ...Ciliates are very important components in most marine ecosystem. They are trophic link between the microbial food web and grazing food chain. In this study, ciliates were collected from 11 sites in the southern South China Sea （SCS） during August 25 to September 28, 2011. Their composition and distribution at the surface and 75m deep depth of the ocean were studied. A total of 30 species belonging to 22 genera were identified, and 22 species of 15 genera were Tintinnids. Eutintinnusfraknoii and E. stramentus were the most common species. The other dominants were strombidiids ciliates including Strombidium conicum and S. globosaneum, which were followed by the tide form, Mesodinium pulex. Ciliates abundance ranged from 46 indL-1 to 368 indL-1 in the open sites, 46-368indL-1 at surface and 73-198indL-1 at 75m deep layer. In the Yongshu reef, ciliates abundance ranged from 167indL-1 to 365 ind L-1 in the water colunm, similar to that in Sanya coral reef waters. Ciliates composition showed obvious difference between surface and 75m deep layer at station S2 （P〈0.05）, while no similar result was observed at other sites. At 75m deep layer, salinity was negatively related to mixed layer depth （P〈 0.05）, but positively to chlorophyll a concentration （P〈 0.05）, indicating that the change of vertical mixing in water column influenced vertical distribution of ciliates in the southern SCS.展开更多
Vertical distributions of phytoplankton biomass, compositions and size structure were investigated during the spring-intermonsoon （April 22 to 30） of 2010 along transact 10°N of the Bay of Bengal, northern Indi...Vertical distributions of phytoplankton biomass, compositions and size structure were investigated during the spring-intermonsoon （April 22 to 30） of 2010 along transact 10°N of the Bay of Bengal, northern Indian Ocean. Surface phytoplankton biomass （Chl a） was （0.065±0.009） μg/L, being greater than 80% of which was contributed by pico-phytoplankton （〈3 μm）. The Chl a concen- tration vertically increased to the maximal values at deep chlorophyll maximum （DCM） layer that shoaled eastwards from 75 to 40 m. The Chl a biomass at DCM layer generally varied between 0.2 and 0.4 μg/L, reaching the maximum of 0.56 μg/L with micro-phytoplankton cells （~20 #m） accounting for 58% and nano- （3-20μm） or pico-cells for 15% and 27%, respectively. In particular, the cells concentration coupling well with phosphate level was observed at middle layer （75-100 m） of 87° to 89°E, dominated by micro-cells diatoms （e.g., Chaetoceros atlanticus v. neapolitana, Chaetoceros femur and Pseudonitzsehia sp.） and cyanobacteria （i.e., Trichodesmium hildebrandtii）, With the ceils concentration reached as high as 4.0 × 10^4 and 4.3 × 10^4 cells/L. At the rest of the trans- act however, dinoflagellates （e.g., Amphisdinium carterae and Prorocentrum sp.） were the dominant species, with the cells concentration varying from 0.3×10^3 to 6.8×10^3 cells/L. Our results also indicate that the regulation of large cells （micro-, nano-） on phytoplankton biomass merely occurred at DCM layer of the Bay.展开更多
采用网络药理学合并分子对接的方法预测五味子治疗急性肝损伤的作用机制.在TCMSP和SwissADME数据库中筛选有效成分,使用PharmMapper数据库预测潜在作用靶点并利用Uniprot数据库匹配基因名.利用OMIM、Genecards、NCBI数据库预测急性肝损...采用网络药理学合并分子对接的方法预测五味子治疗急性肝损伤的作用机制.在TCMSP和SwissADME数据库中筛选有效成分,使用PharmMapper数据库预测潜在作用靶点并利用Uniprot数据库匹配基因名.利用OMIM、Genecards、NCBI数据库预测急性肝损伤靶点,与药物靶点取交集并构建药物-靶点-疾病网络.将交集靶点导入STRING数据库进行PPI分析并找出核心靶点基因.通过DAVID数据库进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)和基因本体(Gene Ontology, GO)富集分析,根据KEGG结果构建药物-靶点-通路-疾病网络,并将所有网络图导入Cytoscape软件进行可视性优化.从TCMSP数据库中下载有效成分结构文件,从RCSB PDB数据库下载靶点蛋白骨架,利用Python和AutoDock系列软件进行分子对接验证.共筛选出交集靶点50个,并根据网络互作结果综合分析,药物治疗疾病主要有效成分可能为去氧紫草素、蚕豆酮等;核心靶点有MAPK1、MAPK8、MAPK14、CASP3、PTGS2、AR等;主要涉及PI3K-Akt、FoxO、TNF等信号通路;分子对接各成分与蛋白骨架结合状态良好.五味子通过去氧紫草素、蚕豆酮等有效成分作用于MAPK1、MAPK8、MAPK14、CASP3、PTGS2、AR等靶点来治疗急性肝损伤,调控过程可能和PI3K-Akt、FoxO、TNF等信号通路有关.展开更多
In the current study,tea saponin,identified as the primary bioactive constituent in seed pomace of Camellia oleifera Abel.,was meticulously extracted and hydrolyzed to yield five known sapogenins:16-O-tiglogycamelliag...In the current study,tea saponin,identified as the primary bioactive constituent in seed pomace of Camellia oleifera Abel.,was meticulously extracted and hydrolyzed to yield five known sapogenins:16-O-tiglogycamelliagnin B(a),camelliagnin A(b),16-O-angeloybarringtogenol C(c),theasapogenol E(d),theasapogenol F(e).Subsequent biotransformation of compound a facilitated the isolation of six novel metabolites(a1−a6).The anti-inflammatory potential of these compounds was assessed using pathogenassociated molecular patterns(PAMPs)and damage-associated molecular patterns molecules(DAMPs)-mediated cellular inflammation models.Notably,compounds b and a2 demonstrated significant inhibitory effects on both lipopolysaccharide(LPS)and high-mobility group box 1(HMGB1)-induced inflammation,surpassing the efficacy of the standard anti-inflammatory agent,carbenoxolone.Conversely,compounds d,a3,and a6 selectivity targeted endogenous HMGB1-induced inflammation,showcasing a pronounced specificity.These results underscore the therapeutic promise of C.oleifera seed pomace-derived compounds as potent agents for the management of inflammatory diseases triggered by infections and tissue damage.展开更多
Cell death is typically defined either as apoptosis or necrosis. Because the consequences of apoptosis and necrosis are quite different for an entire organism, the investigation of the cell-death-mode switch has consi...Cell death is typically defined either as apoptosis or necrosis. Because the consequences of apoptosis and necrosis are quite different for an entire organism, the investigation of the cell-death-mode switch has considerable clinical significance. The existence of a necrosis-to-apoptosis switch induced by hydrogen peroxide in macrophage cell line RAW 264.7 cells was confirmed by using flow cytometry and fluorescence microscopy. With the help of computational simulations, this study predicted that negative feedbacks between NF-κB and MAPKs are implicated in converting necrosis into apoptosis in macrophages exposed to hydrogen peroxide, which has significant implications.展开更多
基金The National Natural Science Foundation of China under contract Nos 41130855,41206132 and 41276162the Natural Science Foundation of Guangdong under contract No.S2011040000151+2 种基金CAS Knowledge Innovation Program under contract No.SQ201115National Project of Sciences and Technology under contract No.2008FY110100CAS Strategic Pilot Science and Technology under contract No.XDA05030403
文摘The Strait of Malacca （SoM）, the world＇s busiest sea-route, is increasingly polluted as the rapid develop- ment of world trades, affecting phytoplankton primary productivity therein. The variations of surface phy- toplankton biomass, size-structure and carbon fixation were investigated across the SoM during the spring period （May 4 to 9, 2011）. Chlorophyll a concentration increased from 0.12 ptg/L at the northwest entrance of the SoM to a maximal 0.63 #g/L at narrowest section, and decreased to 0.10/.tg/L at the southeast entrance. Photosynthetic carbon fixation by phytoplankton coincided well with Chl a biomass, and increased from 10.8 to 22.3 pg C/（L.d）, then decreased to 9.21/zg C/（L.d）; while the carbon fixation rate showed an inverse pattern to the changes of Chl a, and decreased from 87.1 to 35.5 #g C/（#g Chl a.d） and increased thereafter to 95.3 btg C/（/2g Chl a.d）. Picophytoplankton cells （〈3/2m） contributed to more than 60% and 50% of the total Chl a and carbon fixation at both the entry waters; while the contributions of pico-cells decreased sharply to the minimum of 18.3% and 27.5% at the narrowest part of the SoM. In particular, our results showed that the silicate concentration positively regulated Chl a biomass and carbon fixation, reflecting that the higher silicate favoured the growth of phytoplankton and thus led to higher primary production in this strait.
基金supported by the National Basic Research Program of China (2014CB441500)Special Fund for Agro-scientific Research in the Public Interest (201403008)Major Project of Ministry of Agriculture (NFZX2013)
文摘Ciliates are very important components in most marine ecosystem. They are trophic link between the microbial food web and grazing food chain. In this study, ciliates were collected from 11 sites in the southern South China Sea （SCS） during August 25 to September 28, 2011. Their composition and distribution at the surface and 75m deep depth of the ocean were studied. A total of 30 species belonging to 22 genera were identified, and 22 species of 15 genera were Tintinnids. Eutintinnusfraknoii and E. stramentus were the most common species. The other dominants were strombidiids ciliates including Strombidium conicum and S. globosaneum, which were followed by the tide form, Mesodinium pulex. Ciliates abundance ranged from 46 indL-1 to 368 indL-1 in the open sites, 46-368indL-1 at surface and 73-198indL-1 at 75m deep layer. In the Yongshu reef, ciliates abundance ranged from 167indL-1 to 365 ind L-1 in the water colunm, similar to that in Sanya coral reef waters. Ciliates composition showed obvious difference between surface and 75m deep layer at station S2 （P〈0.05）, while no similar result was observed at other sites. At 75m deep layer, salinity was negatively related to mixed layer depth （P〈 0.05）, but positively to chlorophyll a concentration （P〈 0.05）, indicating that the change of vertical mixing in water column influenced vertical distribution of ciliates in the southern SCS.
基金The Key Innovation Group Project of Chinese Academy of Sciences under contract No.KZCX2-YW-Q07the National Natural Science Foundation of China under contract No.41130855+5 种基金the Natural Science Foundation of Guangdong under contract No.S2011040000151CAS Knowledge Innovation Program under contract No.SQ20115National Project of Sciences and Technology under contract No.2008FY110100CAS Strategic Pilot Science and Technology under contract No.XDA05030403MEL Young Scientist Visiting Fellowship of State Key Laboratory of Marine Environment ScienceXiamen University under contract No.MELRS 1006
文摘Vertical distributions of phytoplankton biomass, compositions and size structure were investigated during the spring-intermonsoon （April 22 to 30） of 2010 along transact 10°N of the Bay of Bengal, northern Indian Ocean. Surface phytoplankton biomass （Chl a） was （0.065±0.009） μg/L, being greater than 80% of which was contributed by pico-phytoplankton （〈3 μm）. The Chl a concen- tration vertically increased to the maximal values at deep chlorophyll maximum （DCM） layer that shoaled eastwards from 75 to 40 m. The Chl a biomass at DCM layer generally varied between 0.2 and 0.4 μg/L, reaching the maximum of 0.56 μg/L with micro-phytoplankton cells （~20 #m） accounting for 58% and nano- （3-20μm） or pico-cells for 15% and 27%, respectively. In particular, the cells concentration coupling well with phosphate level was observed at middle layer （75-100 m） of 87° to 89°E, dominated by micro-cells diatoms （e.g., Chaetoceros atlanticus v. neapolitana, Chaetoceros femur and Pseudonitzsehia sp.） and cyanobacteria （i.e., Trichodesmium hildebrandtii）, With the ceils concentration reached as high as 4.0 × 10^4 and 4.3 × 10^4 cells/L. At the rest of the trans- act however, dinoflagellates （e.g., Amphisdinium carterae and Prorocentrum sp.） were the dominant species, with the cells concentration varying from 0.3×10^3 to 6.8×10^3 cells/L. Our results also indicate that the regulation of large cells （micro-, nano-） on phytoplankton biomass merely occurred at DCM layer of the Bay.
文摘采用网络药理学合并分子对接的方法预测五味子治疗急性肝损伤的作用机制.在TCMSP和SwissADME数据库中筛选有效成分,使用PharmMapper数据库预测潜在作用靶点并利用Uniprot数据库匹配基因名.利用OMIM、Genecards、NCBI数据库预测急性肝损伤靶点,与药物靶点取交集并构建药物-靶点-疾病网络.将交集靶点导入STRING数据库进行PPI分析并找出核心靶点基因.通过DAVID数据库进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)和基因本体(Gene Ontology, GO)富集分析,根据KEGG结果构建药物-靶点-通路-疾病网络,并将所有网络图导入Cytoscape软件进行可视性优化.从TCMSP数据库中下载有效成分结构文件,从RCSB PDB数据库下载靶点蛋白骨架,利用Python和AutoDock系列软件进行分子对接验证.共筛选出交集靶点50个,并根据网络互作结果综合分析,药物治疗疾病主要有效成分可能为去氧紫草素、蚕豆酮等;核心靶点有MAPK1、MAPK8、MAPK14、CASP3、PTGS2、AR等;主要涉及PI3K-Akt、FoxO、TNF等信号通路;分子对接各成分与蛋白骨架结合状态良好.五味子通过去氧紫草素、蚕豆酮等有效成分作用于MAPK1、MAPK8、MAPK14、CASP3、PTGS2、AR等靶点来治疗急性肝损伤,调控过程可能和PI3K-Akt、FoxO、TNF等信号通路有关.
基金supported by the National Nature Science Foundation of China(No.21302052)the“Program for New Century Excellent Talents in University”awarded to ZHANG Jian(No.NECT-11-0739)+1 种基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.SJKY19_0658)Jiangsu Funding Program for Excellent Postdoctoral Talent,and“Jiangsu Funding Program for Excellent Postdoctoral Talent”awarded to SHEN Pingping.
文摘In the current study,tea saponin,identified as the primary bioactive constituent in seed pomace of Camellia oleifera Abel.,was meticulously extracted and hydrolyzed to yield five known sapogenins:16-O-tiglogycamelliagnin B(a),camelliagnin A(b),16-O-angeloybarringtogenol C(c),theasapogenol E(d),theasapogenol F(e).Subsequent biotransformation of compound a facilitated the isolation of six novel metabolites(a1−a6).The anti-inflammatory potential of these compounds was assessed using pathogenassociated molecular patterns(PAMPs)and damage-associated molecular patterns molecules(DAMPs)-mediated cellular inflammation models.Notably,compounds b and a2 demonstrated significant inhibitory effects on both lipopolysaccharide(LPS)and high-mobility group box 1(HMGB1)-induced inflammation,surpassing the efficacy of the standard anti-inflammatory agent,carbenoxolone.Conversely,compounds d,a3,and a6 selectivity targeted endogenous HMGB1-induced inflammation,showcasing a pronounced specificity.These results underscore the therapeutic promise of C.oleifera seed pomace-derived compounds as potent agents for the management of inflammatory diseases triggered by infections and tissue damage.
基金supported by the National Natural Science Foundation of China (Grant No. 30870588)the Science Fund for Creative Research Groups (Grant No. 30821006)the Program for New Century Excellent Talents in University (Grant No. NCET-06-0445)
文摘Cell death is typically defined either as apoptosis or necrosis. Because the consequences of apoptosis and necrosis are quite different for an entire organism, the investigation of the cell-death-mode switch has considerable clinical significance. The existence of a necrosis-to-apoptosis switch induced by hydrogen peroxide in macrophage cell line RAW 264.7 cells was confirmed by using flow cytometry and fluorescence microscopy. With the help of computational simulations, this study predicted that negative feedbacks between NF-κB and MAPKs are implicated in converting necrosis into apoptosis in macrophages exposed to hydrogen peroxide, which has significant implications.
