通过系统梳理Web of Science中的相关文献,在辨析探索与利用悖论关系和双元概念的基础上,提出双元的三个内涵,分别为适应外部变动的环境、使探索与利用在高水平上共存、通过探索与利用的相对平衡与交互作用获得组织的灵活性。归纳并比...通过系统梳理Web of Science中的相关文献,在辨析探索与利用悖论关系和双元概念的基础上,提出双元的三个内涵,分别为适应外部变动的环境、使探索与利用在高水平上共存、通过探索与利用的相对平衡与交互作用获得组织的灵活性。归纳并比较双元的实现路径,发现时序型双元和结构型双元是对立与冲突视角下对探索与利用的权衡,而情境型双元是悖论视角下对探索与利用的平衡,情境型双元是实现多层次和嵌套型双元的基础。此外,系统分析情境型双元的五个前因,分别为人力资源实践、组织文化、高管团队行为整合、智力资本和组织情境。以拓展对探索与利用悖论和双元的认知,展现情境型双元的演化路径、理论全貌和实现机理,帮助企业解决创新困境。展开更多
From 2007 to 2017,large-scale green tides occurred every year in the Yellow Sea of China,and Ulva prolifera was the main species leading to the green tide.In this study,we used the Polymerase chain reaction and 3'...From 2007 to 2017,large-scale green tides occurred every year in the Yellow Sea of China,and Ulva prolifera was the main species leading to the green tide.In this study,we used the Polymerase chain reaction and 3'Rapid-amplification of c DNA ends technique to amplify the nr DNA-LSU and IGS sequences in U.prolifera and one species of Blidingia.These techniques showed 3259 bp of nrD NA-LSU and 3388 bp of IGS in U.prolifera and 3282 bp nr DNA-LSU and 3059 bp IGS in Blidingia sp.At the same time,tandem repeats,short dyads,palindromic and multiple simple repeat sequences in the IGS sequence were found by analyzing the structure of the IGS sequence in U.prolifera and Blidingia sp.G+C contents of the IGS sequence in U.prolifera and Blidingia sp.were 52.42% and 53.09%,respectively.We divided the U.prolifera into two types according to the morphological characteristics.Although the specimens of U.prolifera from the Qingdao coastal area,Jiangsu coastal area and the Yellow Sea have different morphologies,their ITS and IGS sequences are almost identical.Therefore,the main species of the green tides in the Qingdao coastal area,Jiangsu coastal area and the Yellow Sea are the same and have the same origin.展开更多
The rbcL gene of Ulva pertusa,Enteromorpha prolifera and Monostroma grevillei was amplified,sequenced and analyzed.By comparing the rbcL sequences with seven other Ulvales species retrieved from GenBank,the sequence d...The rbcL gene of Ulva pertusa,Enteromorpha prolifera and Monostroma grevillei was amplified,sequenced and analyzed.By comparing the rbcL sequences with seven other Ulvales species retrieved from GenBank,the sequence divergences and thephyletic evolution were analyzed and the phylogenetic tree was constructed.From the phylogenetic tree,it can be found that U.per-tusa,E.prolifera and U.californica group in one branch,while E.compressa,U.rigida and U.fenestrata cluster in another clade.Obviously,unlike the Enteomorpha species,the Ulva species do not gather in one branch.Therefore Ulva and Enteomorpha might beaffiliates of one genus.E.compressa and E.intestinalis gathered together,which coincided with the morphological characters.However,the thallus of U.pertusa is thick and with many holes,which is different from E.prolifera in morphology.They cluster togetherin the phylogenetic tree with a genetic distance of 0.005.The results indicate that Ulva and Enteromorpha are not distinguishedstrictly.展开更多
Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain l...Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis.展开更多
文摘通过系统梳理Web of Science中的相关文献,在辨析探索与利用悖论关系和双元概念的基础上,提出双元的三个内涵,分别为适应外部变动的环境、使探索与利用在高水平上共存、通过探索与利用的相对平衡与交互作用获得组织的灵活性。归纳并比较双元的实现路径,发现时序型双元和结构型双元是对立与冲突视角下对探索与利用的权衡,而情境型双元是悖论视角下对探索与利用的平衡,情境型双元是实现多层次和嵌套型双元的基础。此外,系统分析情境型双元的五个前因,分别为人力资源实践、组织文化、高管团队行为整合、智力资本和组织情境。以拓展对探索与利用悖论和双元的认知,展现情境型双元的演化路径、理论全貌和实现机理,帮助企业解决创新困境。
基金supported by the National Key R&D Program of China (Nos. 2016YFC1402102 and 2016YFC 1402104)the National Natural Science Foundation of China (No. 41276134)
文摘From 2007 to 2017,large-scale green tides occurred every year in the Yellow Sea of China,and Ulva prolifera was the main species leading to the green tide.In this study,we used the Polymerase chain reaction and 3'Rapid-amplification of c DNA ends technique to amplify the nr DNA-LSU and IGS sequences in U.prolifera and one species of Blidingia.These techniques showed 3259 bp of nrD NA-LSU and 3388 bp of IGS in U.prolifera and 3282 bp nr DNA-LSU and 3059 bp IGS in Blidingia sp.At the same time,tandem repeats,short dyads,palindromic and multiple simple repeat sequences in the IGS sequence were found by analyzing the structure of the IGS sequence in U.prolifera and Blidingia sp.G+C contents of the IGS sequence in U.prolifera and Blidingia sp.were 52.42% and 53.09%,respectively.We divided the U.prolifera into two types according to the morphological characteristics.Although the specimens of U.prolifera from the Qingdao coastal area,Jiangsu coastal area and the Yellow Sea have different morphologies,their ITS and IGS sequences are almost identical.Therefore,the main species of the green tides in the Qingdao coastal area,Jiangsu coastal area and the Yellow Sea are the same and have the same origin.
基金supported by the National Natural Scientific Foundations of China(Grant No.30570125)the Key Construction Laboratory of Marine Biotechnology of Jiangsu Province(Grant No.2005HS006)
文摘The rbcL gene of Ulva pertusa,Enteromorpha prolifera and Monostroma grevillei was amplified,sequenced and analyzed.By comparing the rbcL sequences with seven other Ulvales species retrieved from GenBank,the sequence divergences and thephyletic evolution were analyzed and the phylogenetic tree was constructed.From the phylogenetic tree,it can be found that U.per-tusa,E.prolifera and U.californica group in one branch,while E.compressa,U.rigida and U.fenestrata cluster in another clade.Obviously,unlike the Enteomorpha species,the Ulva species do not gather in one branch.Therefore Ulva and Enteomorpha might beaffiliates of one genus.E.compressa and E.intestinalis gathered together,which coincided with the morphological characters.However,the thallus of U.pertusa is thick and with many holes,which is different from E.prolifera in morphology.They cluster togetherin the phylogenetic tree with a genetic distance of 0.005.The results indicate that Ulva and Enteromorpha are not distinguishedstrictly.
基金supported by a grant from the PhD fellowship program of Soochow Universitythe National Natural Science Foundation of China(No.41276134)
文摘Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis.
