以稗草和千金子为供试杂草,以南梗9108、南京11、K两优10号为供试水稻,通过室内整株生测法研究助剂GY-Tmax对双草醚、氰氟草酯防效和对水稻安全性的影响。结果表明,添加90 mL/667 m^2助剂GY-Tmax后,双草醚对3~4叶期稗草的ED90由3. 651 1...以稗草和千金子为供试杂草,以南梗9108、南京11、K两优10号为供试水稻,通过室内整株生测法研究助剂GY-Tmax对双草醚、氰氟草酯防效和对水稻安全性的影响。结果表明,添加90 mL/667 m^2助剂GY-Tmax后,双草醚对3~4叶期稗草的ED90由3. 651 1 g a. i./667 m^2降至0. 932 9 g a. i./667 m^2,这表明助剂GY-Tmax明显增加了双草醚对稗草的防效;双草醚对千金子的防效较差,且助剂GY-Tmax对双草醚防除千金子无明显增效作用。添加90 mL/667 m^2助剂GY-Tmax后,氰氟草酯对3~4叶期稗草和千金子的防效均有所提高,氰氟草酯对稗草的ED90由10. 903 5 g a. i./667 m^2降至5. 231 1 g a. i./667 m^2,对千金子的ED_(90)由6. 840 0 g a. i./667 m^2降至2. 873 6 g a. i./667 m^2。添加90 mL/667 m^2助剂GY-Tmax后,双草醚和氰氟草酯对南梗9108、南京11、K两优10号水稻的安全性与不添加助剂时相当。展开更多
Lipoxygenase （LOX, EC1.13.11.12） is a key enzyme during the degradation of lipids in animals and even plants, and also the first key enzyme responsible for the biosynthesis of jasmonate. To purify and characterize t...Lipoxygenase （LOX, EC1.13.11.12） is a key enzyme during the degradation of lipids in animals and even plants, and also the first key enzyme responsible for the biosynthesis of jasmonate. To purify and characterize the OsLOX1 gene from rice seeds, the entire coding region of the OsLOX1 gene was inserted into an expression vector pET30a（＋） and transformed into Escherichia coil BL21 （DE3）. Expression of the fusion protein was successfully induced by isopropyl-β-D- thiogalactopyranoside （IPTG） and the purified recombinant protein was obtained by His.Bind Kits. Further assay showed that the purified recombinant protein exhibited the LOX activity. The optimum pH was 4.8 （acetate buffer） and the optimum temperature was 30℃ for the above enzyme. Thus, the recombinant might confer an available usage for the synthesis of jasmonate in vitro, and also provides a possibility for elucidating the inter-relationship between the primary structure of the plant seed lipoxygenase protein and its physiological functions.展开更多
文摘以稗草和千金子为供试杂草,以南梗9108、南京11、K两优10号为供试水稻,通过室内整株生测法研究助剂GY-Tmax对双草醚、氰氟草酯防效和对水稻安全性的影响。结果表明,添加90 mL/667 m^2助剂GY-Tmax后,双草醚对3~4叶期稗草的ED90由3. 651 1 g a. i./667 m^2降至0. 932 9 g a. i./667 m^2,这表明助剂GY-Tmax明显增加了双草醚对稗草的防效;双草醚对千金子的防效较差,且助剂GY-Tmax对双草醚防除千金子无明显增效作用。添加90 mL/667 m^2助剂GY-Tmax后,氰氟草酯对3~4叶期稗草和千金子的防效均有所提高,氰氟草酯对稗草的ED90由10. 903 5 g a. i./667 m^2降至5. 231 1 g a. i./667 m^2,对千金子的ED_(90)由6. 840 0 g a. i./667 m^2降至2. 873 6 g a. i./667 m^2。添加90 mL/667 m^2助剂GY-Tmax后,双草醚和氰氟草酯对南梗9108、南京11、K两优10号水稻的安全性与不添加助剂时相当。
基金grants from the National Basic Research Program of China (Grant No. 2004CB2117204)the National High-tech Research and Development Program of China (Grant No. 2006AA100101)+1 种基金the National Program of Science Technology and Tackle Key Problem of Chinathe Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) of China
文摘Lipoxygenase （LOX, EC1.13.11.12） is a key enzyme during the degradation of lipids in animals and even plants, and also the first key enzyme responsible for the biosynthesis of jasmonate. To purify and characterize the OsLOX1 gene from rice seeds, the entire coding region of the OsLOX1 gene was inserted into an expression vector pET30a（＋） and transformed into Escherichia coil BL21 （DE3）. Expression of the fusion protein was successfully induced by isopropyl-β-D- thiogalactopyranoside （IPTG） and the purified recombinant protein was obtained by His.Bind Kits. Further assay showed that the purified recombinant protein exhibited the LOX activity. The optimum pH was 4.8 （acetate buffer） and the optimum temperature was 30℃ for the above enzyme. Thus, the recombinant might confer an available usage for the synthesis of jasmonate in vitro, and also provides a possibility for elucidating the inter-relationship between the primary structure of the plant seed lipoxygenase protein and its physiological functions.
