Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N gene.Methods Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N ...Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N gene.Methods Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific primers.The amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into E.coli XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive clones.Finally,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N genome.Results The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids （20 kinds）,the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% respectively.The evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic regionality.Conclusion The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention control.展开更多
目前,关于膝关节自发性骨坏死(Spontaneous Osteonecrosis of the Knee,SONK)机制尚缺乏清晰的认识,西医提出的机制主要是创伤相关理论和血管源性理论,中医学尚无此相关病因病机制的记载。明确本病机制对截断病程进展,有效治疗SONK意义...目前,关于膝关节自发性骨坏死(Spontaneous Osteonecrosis of the Knee,SONK)机制尚缺乏清晰的认识,西医提出的机制主要是创伤相关理论和血管源性理论,中医学尚无此相关病因病机制的记载。明确本病机制对截断病程进展,有效治疗SONK意义巨大,笔者检索国内外文献,并总结、归纳,现将SONK中、西医相关发病机制做如下综述,希望能对临床骨科医师诊治SONK提供一定的理论依据和指导。展开更多
基金supported by the science foundation of the Health Department of Hunan Province (Grant No A2007008 and B2009-088)the National Department Public Benefit Research Foundation (Grant No 200803014)
文摘Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N gene.Methods Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific primers.The amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into E.coli XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive clones.Finally,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N genome.Results The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids （20 kinds）,the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% respectively.The evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic regionality.Conclusion The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention control.
文摘目前,关于膝关节自发性骨坏死(Spontaneous Osteonecrosis of the Knee,SONK)机制尚缺乏清晰的认识,西医提出的机制主要是创伤相关理论和血管源性理论,中医学尚无此相关病因病机制的记载。明确本病机制对截断病程进展,有效治疗SONK意义巨大,笔者检索国内外文献,并总结、归纳,现将SONK中、西医相关发病机制做如下综述,希望能对临床骨科医师诊治SONK提供一定的理论依据和指导。
