目的:探讨高脂饮食对大鼠海马、大脑皮质和脊髓胶质细胞源性神经营养因子(GDNF)的影响。方法:20只雄性SD大鼠随机分成两组:普通饮食组(ND)和高脂饮食组(HFD),每组10只,分别给子两组大鼠普通饮食和高脂饮食14周,测量各组大鼠体重脂肪重、...目的:探讨高脂饮食对大鼠海马、大脑皮质和脊髓胶质细胞源性神经营养因子(GDNF)的影响。方法:20只雄性SD大鼠随机分成两组:普通饮食组(ND)和高脂饮食组(HFD),每组10只,分别给子两组大鼠普通饮食和高脂饮食14周,测量各组大鼠体重脂肪重、Lee's指数和脂体比。应用real time RT-PCR检测各组大鼠海马、大脑皮质和脊髓GDNFmRNA的表达;应用Western Blot检测各组大鼠海马、大脑皮质和脊髓CDNF蛋白的表达;应用酶联免疫吸附实验(ELISA)检测各组大鼠海马、大脑皮质和脊髓肿瘤坏死因子-α(TNF-α)、白细胞介索-1β(IL-1β)和IL-6的水平。结果:高脂饮食导致大鼠体重、脂肪重,Lee's指数和脂体比均明显升高(P<0.05);HFD组大鼠海马、大脑皮质和脊髓GDNF mRNA表达水平较ND组均显著下降(P<0.01),HFD组大鼠海马、大脑皮质和脊髓GDNF蛋白表达水平较ND组均明显下降(P<0.05);HFD组大鼠海马、大脑皮质和脊髓中TNF-α、IL-1β和IL-6水平与ND组相比均明显升高(P<0.01)。结论:高脂饮食造成大鼠肥胖,降低了大鼠海马、大脑皮质和脊髓GDNF的表达水平,同时促进了炎症反应的发生。展开更多
目的:通过高脂饮食喂养建立肥胖大鼠模型,探讨低氧训练对肥胖大鼠大脑皮质脑源性神经营养因子(BDNF)及其受体酪氨酸激酶受体B(TrkB)的影响,为低氧训练改善肥胖大鼠神经系统功能提供理论依据。方法:32只3周龄雄性SD大鼠,高脂饲料喂养10...目的:通过高脂饮食喂养建立肥胖大鼠模型,探讨低氧训练对肥胖大鼠大脑皮质脑源性神经营养因子(BDNF)及其受体酪氨酸激酶受体B(TrkB)的影响,为低氧训练改善肥胖大鼠神经系统功能提供理论依据。方法:32只3周龄雄性SD大鼠,高脂饲料喂养10周建立肥胖模型,随机分为常氧安静组(NC组)、常氧训练组(NT组)、低氧安静组(HC组)、低氧训练组(HT组)。NC组和NT组统称为常氧组(N组),HC组和HT组统称为低氧组(H组),NC组和HC组统称为安静组(C组),NT组和HT组统称为训练组(T组)。低氧环境氧气浓度为13.6%,训练组用水平动物跑台进行耐力训练,常氧组训练强度为25 m/min,低氧组训练强度为20 m/min,持续运动1 h/天、5天/周,共4周。应用RT-qPCR检测各组大鼠大脑皮质BDNF、TrkB mRNA表达水平;应用Western-Blot检测大鼠大脑皮质BDNF、TrkB蛋白以及磷酸化TrkB蛋白(p-TrkB)的表达;应用ELISA检测大鼠大脑皮质肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的水平。结果:1)T组大鼠大脑皮质BDNF和TrkB m RNA表达较C组显著上升(P<0.01),H组较N组显著上升(P<0.05),NT组较NC组显著上升(P<0.01),HT组较HC组和NT组显著上升(P<0.01);2)T组大脑皮质BDNF蛋白表达较C组显著上升(P<0.01),H组和N组无显著性差异;各组大鼠大脑皮质TrkB蛋白表达水平无显著差异;T组p-TrkB蛋白表达较C组显著上升(P<0.01),H组和N组无显著性差异;3)T组TNF-α、IL-1β和IL-6表达较C组显著下降(P<0.05),H组和N组无显著性差异。结论:运动训练能减少肥胖大鼠大脑皮质炎症因子TNF-α、IL-1β和IL-6的分泌,促进大脑皮质BDNF及其受体TrkB的表达,从而提高神经营养作用。低氧训练在提高肥胖大鼠大脑皮质BDNF和TrkB m RNA的表达上较常氧训练更具优势。展开更多
In order to discuss the geochemical characteristic of REEs(rare earth elements) and their geological application,we measured the contents of rare earth elements,trace elements and minerals of 29 Lopingian(Late Permian...In order to discuss the geochemical characteristic of REEs(rare earth elements) and their geological application,we measured the contents of rare earth elements,trace elements and minerals of 29 Lopingian(Late Permian) mudstone samples in Panxian county,carrying out ICP-MS and XRD analysis.The results show that the amount of REEs(185.56-729.46 10à6) is high.The ratios of w(LREE)/w(HREE)(6.84-13.86) and w(La)N/w(Yb)N(1.01-3.02) show clear differentiation of LREEs and HREEs.RREE has a significantly or critically positive correlation with lithophile elements Th,Nb,Ta,Ti,Ga,Sc,Cs,Zr,Hf,Sr,Be and chalcophile element Zn,a critically negative correlation with siderophile element Fe and a slightly positive correlation with illite,illite smectite mixed layers and siderite.REEs originate mainly from terrigenous minerals,in an inorganic phase.Source rocks of our samples consist of Emeishan basalt and a small part of sedimentary rocks,as suggested by the distribution patterns of REEs and w(PREE)-w(La)/w(Yb) diagram.Moreover,abnormal surfaces near the sequence boundaries(SB2,SB3,SB4) are related with the boundaries,identifled by geochemical characteristics of the REEs,such as PREE,w(LREE)/w(HREE),Eu/Eu and Ceanom.展开更多
文摘目的:探讨高脂饮食对大鼠海马、大脑皮质和脊髓胶质细胞源性神经营养因子(GDNF)的影响。方法:20只雄性SD大鼠随机分成两组:普通饮食组(ND)和高脂饮食组(HFD),每组10只,分别给子两组大鼠普通饮食和高脂饮食14周,测量各组大鼠体重脂肪重、Lee's指数和脂体比。应用real time RT-PCR检测各组大鼠海马、大脑皮质和脊髓GDNFmRNA的表达;应用Western Blot检测各组大鼠海马、大脑皮质和脊髓CDNF蛋白的表达;应用酶联免疫吸附实验(ELISA)检测各组大鼠海马、大脑皮质和脊髓肿瘤坏死因子-α(TNF-α)、白细胞介索-1β(IL-1β)和IL-6的水平。结果:高脂饮食导致大鼠体重、脂肪重,Lee's指数和脂体比均明显升高(P<0.05);HFD组大鼠海马、大脑皮质和脊髓GDNF mRNA表达水平较ND组均显著下降(P<0.01),HFD组大鼠海马、大脑皮质和脊髓GDNF蛋白表达水平较ND组均明显下降(P<0.05);HFD组大鼠海马、大脑皮质和脊髓中TNF-α、IL-1β和IL-6水平与ND组相比均明显升高(P<0.01)。结论:高脂饮食造成大鼠肥胖,降低了大鼠海马、大脑皮质和脊髓GDNF的表达水平,同时促进了炎症反应的发生。
文摘目的:通过高脂饮食喂养建立肥胖大鼠模型,探讨低氧训练对肥胖大鼠大脑皮质脑源性神经营养因子(BDNF)及其受体酪氨酸激酶受体B(TrkB)的影响,为低氧训练改善肥胖大鼠神经系统功能提供理论依据。方法:32只3周龄雄性SD大鼠,高脂饲料喂养10周建立肥胖模型,随机分为常氧安静组(NC组)、常氧训练组(NT组)、低氧安静组(HC组)、低氧训练组(HT组)。NC组和NT组统称为常氧组(N组),HC组和HT组统称为低氧组(H组),NC组和HC组统称为安静组(C组),NT组和HT组统称为训练组(T组)。低氧环境氧气浓度为13.6%,训练组用水平动物跑台进行耐力训练,常氧组训练强度为25 m/min,低氧组训练强度为20 m/min,持续运动1 h/天、5天/周,共4周。应用RT-qPCR检测各组大鼠大脑皮质BDNF、TrkB mRNA表达水平;应用Western-Blot检测大鼠大脑皮质BDNF、TrkB蛋白以及磷酸化TrkB蛋白(p-TrkB)的表达;应用ELISA检测大鼠大脑皮质肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的水平。结果:1)T组大鼠大脑皮质BDNF和TrkB m RNA表达较C组显著上升(P<0.01),H组较N组显著上升(P<0.05),NT组较NC组显著上升(P<0.01),HT组较HC组和NT组显著上升(P<0.01);2)T组大脑皮质BDNF蛋白表达较C组显著上升(P<0.01),H组和N组无显著性差异;各组大鼠大脑皮质TrkB蛋白表达水平无显著差异;T组p-TrkB蛋白表达较C组显著上升(P<0.01),H组和N组无显著性差异;3)T组TNF-α、IL-1β和IL-6表达较C组显著下降(P<0.05),H组和N组无显著性差异。结论:运动训练能减少肥胖大鼠大脑皮质炎症因子TNF-α、IL-1β和IL-6的分泌,促进大脑皮质BDNF及其受体TrkB的表达,从而提高神经营养作用。低氧训练在提高肥胖大鼠大脑皮质BDNF和TrkB m RNA的表达上较常氧训练更具优势。
基金supported by the Key Program of the National Natural Science Foundation of China (No. 40730422)
文摘In order to discuss the geochemical characteristic of REEs(rare earth elements) and their geological application,we measured the contents of rare earth elements,trace elements and minerals of 29 Lopingian(Late Permian) mudstone samples in Panxian county,carrying out ICP-MS and XRD analysis.The results show that the amount of REEs(185.56-729.46 10à6) is high.The ratios of w(LREE)/w(HREE)(6.84-13.86) and w(La)N/w(Yb)N(1.01-3.02) show clear differentiation of LREEs and HREEs.RREE has a significantly or critically positive correlation with lithophile elements Th,Nb,Ta,Ti,Ga,Sc,Cs,Zr,Hf,Sr,Be and chalcophile element Zn,a critically negative correlation with siderophile element Fe and a slightly positive correlation with illite,illite smectite mixed layers and siderite.REEs originate mainly from terrigenous minerals,in an inorganic phase.Source rocks of our samples consist of Emeishan basalt and a small part of sedimentary rocks,as suggested by the distribution patterns of REEs and w(PREE)-w(La)/w(Yb) diagram.Moreover,abnormal surfaces near the sequence boundaries(SB2,SB3,SB4) are related with the boundaries,identifled by geochemical characteristics of the REEs,such as PREE,w(LREE)/w(HREE),Eu/Eu and Ceanom.