目的利用ELISA动态检测血浆可溶性Sema4D在体外循环(CPB)手术过程中的含量变化,并初步分析其可能的原因。方法以重组人Sema4D细胞外段作为抗原,制备Sema4D抗体,建立检测人血浆中可溶性Sema4D水平的ELISA方法。收集体外循环患者手术前、...目的利用ELISA动态检测血浆可溶性Sema4D在体外循环(CPB)手术过程中的含量变化,并初步分析其可能的原因。方法以重组人Sema4D细胞外段作为抗原,制备Sema4D抗体,建立检测人血浆中可溶性Sema4D水平的ELISA方法。收集体外循环患者手术前、肝素化后10 min以及中和肝素后10 min 3个时间点的血浆样本,ELISA检测可溶性Sema4D水平在体外循环手术过程中的动态变化。取3个时间点的血液样本,流式检测T细胞和血小板表面Sema4D表达的动态变化。结果建立了适用于检测人外周血血浆可溶性Sema4D水平的夹心ELISA方法。体外循环手术患者血浆中可溶性Sema4D水平在手术开始后显著增加(P<0.01),并持续到手术结束后。肝素化后10 min,75%患者Sema4D高表达的血小板比术前明显增加(P<0.05),而Sema4D高表达的T细胞无明显变化。结论血小板活化释放Sema4D可能是体外循环过程中血浆可溶性Sema4D增加的原因之一。因此血浆可溶性Sema4D水平的变化可能成为体外循环预后的判断指标。展开更多
It is still unclear whether the timing of intracoronary stem cell therapy affects the therapeutic response in patients with myocardial infarction.The natural course of healing the infarction and the presence of putati...It is still unclear whether the timing of intracoronary stem cell therapy affects the therapeutic response in patients with myocardial infarction.The natural course of healing the infarction and the presence of putative homing signals within the damaged myocardium appear to favor cell engraftment during the transendothelial passage in the early days after reperfusion.However,the adverse inflammatory environment,with its high oxidative stress,might be deleterious if cells are administered too early after reperfusion.Here we highlight several aspects of the timing of intracoronary stem cell therapy.Our results showed that transplantation of bone marrow mesenchymal stem cells at 2 4 weeks after myocardial infarction is more favorable for reduction of the scar area,inhibition of left ventricular remodeling,and recovery of heart function.Coronary injection of autologous bone marrow mesenchymal stem cells at 2 4 weeks after acute myocardial infarction is safe and does not increase the incidence of complications.展开更多
文摘目的利用ELISA动态检测血浆可溶性Sema4D在体外循环(CPB)手术过程中的含量变化,并初步分析其可能的原因。方法以重组人Sema4D细胞外段作为抗原,制备Sema4D抗体,建立检测人血浆中可溶性Sema4D水平的ELISA方法。收集体外循环患者手术前、肝素化后10 min以及中和肝素后10 min 3个时间点的血浆样本,ELISA检测可溶性Sema4D水平在体外循环手术过程中的动态变化。取3个时间点的血液样本,流式检测T细胞和血小板表面Sema4D表达的动态变化。结果建立了适用于检测人外周血血浆可溶性Sema4D水平的夹心ELISA方法。体外循环手术患者血浆中可溶性Sema4D水平在手术开始后显著增加(P<0.01),并持续到手术结束后。肝素化后10 min,75%患者Sema4D高表达的血小板比术前明显增加(P<0.05),而Sema4D高表达的T细胞无明显变化。结论血小板活化释放Sema4D可能是体外循环过程中血浆可溶性Sema4D增加的原因之一。因此血浆可溶性Sema4D水平的变化可能成为体外循环预后的判断指标。
文摘It is still unclear whether the timing of intracoronary stem cell therapy affects the therapeutic response in patients with myocardial infarction.The natural course of healing the infarction and the presence of putative homing signals within the damaged myocardium appear to favor cell engraftment during the transendothelial passage in the early days after reperfusion.However,the adverse inflammatory environment,with its high oxidative stress,might be deleterious if cells are administered too early after reperfusion.Here we highlight several aspects of the timing of intracoronary stem cell therapy.Our results showed that transplantation of bone marrow mesenchymal stem cells at 2 4 weeks after myocardial infarction is more favorable for reduction of the scar area,inhibition of left ventricular remodeling,and recovery of heart function.Coronary injection of autologous bone marrow mesenchymal stem cells at 2 4 weeks after acute myocardial infarction is safe and does not increase the incidence of complications.
