目的·探究伴思维形式障碍(formal thought disorder,FTD)的精神分裂症(schizophrenia,SZ)患者、不伴FTD的SZ患者、遗传高危被试及健康对照者在语言加工时事件相关电位中N400、P600成分的差异。方法·选择于上海交通大学医学院...目的·探究伴思维形式障碍(formal thought disorder,FTD)的精神分裂症(schizophrenia,SZ)患者、不伴FTD的SZ患者、遗传高危被试及健康对照者在语言加工时事件相关电位中N400、P600成分的差异。方法·选择于上海交通大学医学院附属精神卫生中心住院的伴FTD的SZ患者30例及不伴FTD的SZ患者29例、遗传高危被试(SZ患者的一级亲属)22例及健康对照者31名作为研究对象。要求上述被试完成4种句子加工任务(语义和句法均正确;语义错误,句法正确;句法错误,语义正确;语义和句法均错误),同时记录脑电数据。采用重复测量方差分析比较4组被试的反应正确率及N400、P600成分的波幅和潜伏期的差异。结果·与健康对照者比较,伴FTD的SZ患者反应正确率降低(P=0.000)。伴FTD的SZ患者未诱发显著的N400成分,其N400波幅与健康对照者比较差异有统计学意义(P=0.003)。4组被试均诱发了P600成分,语义、句法和组别之间存在显著的交互作用(P=0.022);进一步分析发现,伴FTD的SZ患者与不伴FTD的SZ患者P600波幅在句法正确和句法错误时差异无统计学意义;伴FTD的SZ患者P600波幅在语义正确和语义错误时差异无统计学意义,其他3组语义正确条件时的P600波幅大于语义错误时的P600波幅。结论·SZ患者在语义加工水平神经活动异常,伴FTD的SZ患者语义和句法加工均受到损害。展开更多
Objective To explore the molecular mechanism of enhancing the effect of risperidone on the cognitive function of schizophrenic mice by fluoxetine. Methods Schizophrenic mice were injected with M K-801 and drugs( fluox...Objective To explore the molecular mechanism of enhancing the effect of risperidone on the cognitive function of schizophrenic mice by fluoxetine. Methods Schizophrenic mice were injected with M K-801 and drugs( fluoxetine and / or risperidone) were adm inistrated orally 30 min before the injection of M K-801.Mice of the control group adm inistrated the same dose of norm al saline. The cognitive function, distance of activity, and stereotyped behavior of mice were observed. The expressions of key param eters of MEK / ERK pathway in hippocam pus were detected by Western blotting or real-time qPCR. The participation of MEK / ERK pathway in isolated hippocam palneuron injuries induced by M K-801 mediation was also analyzed. Results The mice of M K-801 group showed prolonged activity distance, increased stereotyped behavior scores, and cognitive im pairm ent. Expressions of pMEK/MEK, pERK/ERK1 /2, MMP2 /9, and TIMP1 /2 of the MK-801 group increased. The aforem entioned proteins of drug adm inistration groups decreased dram atically. For schizophrenic m ice, fluoxetine enhanced effects of risperidone in norm alizing abnorm al behaviors, affecting cognitive function, and unusual protein expressions( P < 0. 05). Results of in vitro experim ents were sim ilar to those of in vivo experim ents. Conclusion Fluoxetine enhances the anti-schizophrenic effect of risperidone by norm alizing the activated MEK / ERK signaling pathway.展开更多
文摘目的·探究伴思维形式障碍(formal thought disorder,FTD)的精神分裂症(schizophrenia,SZ)患者、不伴FTD的SZ患者、遗传高危被试及健康对照者在语言加工时事件相关电位中N400、P600成分的差异。方法·选择于上海交通大学医学院附属精神卫生中心住院的伴FTD的SZ患者30例及不伴FTD的SZ患者29例、遗传高危被试(SZ患者的一级亲属)22例及健康对照者31名作为研究对象。要求上述被试完成4种句子加工任务(语义和句法均正确;语义错误,句法正确;句法错误,语义正确;语义和句法均错误),同时记录脑电数据。采用重复测量方差分析比较4组被试的反应正确率及N400、P600成分的波幅和潜伏期的差异。结果·与健康对照者比较,伴FTD的SZ患者反应正确率降低(P=0.000)。伴FTD的SZ患者未诱发显著的N400成分,其N400波幅与健康对照者比较差异有统计学意义(P=0.003)。4组被试均诱发了P600成分,语义、句法和组别之间存在显著的交互作用(P=0.022);进一步分析发现,伴FTD的SZ患者与不伴FTD的SZ患者P600波幅在句法正确和句法错误时差异无统计学意义;伴FTD的SZ患者P600波幅在语义正确和语义错误时差异无统计学意义,其他3组语义正确条件时的P600波幅大于语义错误时的P600波幅。结论·SZ患者在语义加工水平神经活动异常,伴FTD的SZ患者语义和句法加工均受到损害。
基金Special Project from Ministry of Science and Technology of China(2012ZX09303-003)Shanghai Natural Science Foundation(13ZR1435800)Training Project of Excellent Talents in Shanghai(XBR2011049)
文摘Objective To explore the molecular mechanism of enhancing the effect of risperidone on the cognitive function of schizophrenic mice by fluoxetine. Methods Schizophrenic mice were injected with M K-801 and drugs( fluoxetine and / or risperidone) were adm inistrated orally 30 min before the injection of M K-801.Mice of the control group adm inistrated the same dose of norm al saline. The cognitive function, distance of activity, and stereotyped behavior of mice were observed. The expressions of key param eters of MEK / ERK pathway in hippocam pus were detected by Western blotting or real-time qPCR. The participation of MEK / ERK pathway in isolated hippocam palneuron injuries induced by M K-801 mediation was also analyzed. Results The mice of M K-801 group showed prolonged activity distance, increased stereotyped behavior scores, and cognitive im pairm ent. Expressions of pMEK/MEK, pERK/ERK1 /2, MMP2 /9, and TIMP1 /2 of the MK-801 group increased. The aforem entioned proteins of drug adm inistration groups decreased dram atically. For schizophrenic m ice, fluoxetine enhanced effects of risperidone in norm alizing abnorm al behaviors, affecting cognitive function, and unusual protein expressions( P < 0. 05). Results of in vitro experim ents were sim ilar to those of in vivo experim ents. Conclusion Fluoxetine enhances the anti-schizophrenic effect of risperidone by norm alizing the activated MEK / ERK signaling pathway.
