Objective: To explore the association between the polymorphism of the β3-adrenergic receptor(β3-AR )gene and obesity, type 2 diabetes, insulin resistance in Chinese pedigrees. Methods: Eight pedigrees with obesity a...Objective: To explore the association between the polymorphism of the β3-adrenergic receptor(β3-AR )gene and obesity, type 2 diabetes, insulin resistance in Chinese pedigrees. Methods: Eight pedigrees with obesity and type 2 diabetes have been detected with the technique of PCR-RFLP in codon 64 of the exon region representing the variation Trp64 Arg of β3-AR gene. Results: In pedigree linkage analysis, the maximal LOD score of β3-AR gene with obesity was 3.385109 (θ=0.000000) at the mode of autosomal dominant in pedigree 3. The maximal LOD score of β3-AR with type 2 diabetes was 0.222336 (θ=0.000000) at the mode of autosomal dominant in pedigree 2 and 0.805003(θ=0.000000) at the mode of autosomal recessive in pedigree 4. Conclusion: The results indicate that ① The Trp 64 Arg variation have a cause effect significances of some familial obesity. Pedigree linkage analysis can powerfully help to understand the action mechanism of the candidate genes, ②Better comphrehension the workings of adrenergic receptors should provide a new understanding of obesity, type 2 diabetes and insulin resistance and perhaps lead to new methods of treatment.展开更多
A method for solving nonlinear programming using genetic algorithm is presented. In the operations of crossover and mutation in each generation, to ensure the new solutions are all feasible, we present a method in whi...A method for solving nonlinear programming using genetic algorithm is presented. In the operations of crossover and mutation in each generation, to ensure the new solutions are all feasible, we present a method in which the bounds of every variable in the solution are estimated beforehand according to the constrained conditions. For the operation of mutation, we present two methods of cube bounding and variable bounding. The experimental results are given and analyzed. They show that the method is efficient and can obtain the results in less generation.展开更多
文摘Objective: To explore the association between the polymorphism of the β3-adrenergic receptor(β3-AR )gene and obesity, type 2 diabetes, insulin resistance in Chinese pedigrees. Methods: Eight pedigrees with obesity and type 2 diabetes have been detected with the technique of PCR-RFLP in codon 64 of the exon region representing the variation Trp64 Arg of β3-AR gene. Results: In pedigree linkage analysis, the maximal LOD score of β3-AR gene with obesity was 3.385109 (θ=0.000000) at the mode of autosomal dominant in pedigree 3. The maximal LOD score of β3-AR with type 2 diabetes was 0.222336 (θ=0.000000) at the mode of autosomal dominant in pedigree 2 and 0.805003(θ=0.000000) at the mode of autosomal recessive in pedigree 4. Conclusion: The results indicate that ① The Trp 64 Arg variation have a cause effect significances of some familial obesity. Pedigree linkage analysis can powerfully help to understand the action mechanism of the candidate genes, ②Better comphrehension the workings of adrenergic receptors should provide a new understanding of obesity, type 2 diabetes and insulin resistance and perhaps lead to new methods of treatment.
基金The work is supported by National Natural Science Foundation of China ( 6 9974 0 33)
文摘A method for solving nonlinear programming using genetic algorithm is presented. In the operations of crossover and mutation in each generation, to ensure the new solutions are all feasible, we present a method in which the bounds of every variable in the solution are estimated beforehand according to the constrained conditions. For the operation of mutation, we present two methods of cube bounding and variable bounding. The experimental results are given and analyzed. They show that the method is efficient and can obtain the results in less generation.
