目的探讨Pan-TRK免疫组化在涎腺分泌性癌(secretory carcinoma of salivary glands,SCSG)诊断及鉴别诊断中的价值。方法收集14例SCSG临床资料,采用免疫组化、FISH对SCSG进行检测。另选取经典的腺泡细胞癌、黏液表皮样癌、腺样囊性癌各1...目的探讨Pan-TRK免疫组化在涎腺分泌性癌(secretory carcinoma of salivary glands,SCSG)诊断及鉴别诊断中的价值。方法收集14例SCSG临床资料,采用免疫组化、FISH对SCSG进行检测。另选取经典的腺泡细胞癌、黏液表皮样癌、腺样囊性癌各10例作为对照。结果14例SCSG患者男女比为4∶3,年龄15~79岁,10例发生于腮腺,2例发生于颊部,1例发生于腭部,1例发生于颈部;11例生存状态良好,1例复发生存,2例失访。14例SCSG,多呈浸润性生长,呈微囊状、乳头-囊状、管状、实性排列或几者混合,腔内伴丰富分泌物,1例见神经侵犯,1例伴淋巴结转移。免疫表型:14例SCSG中CK7、S-100、Mammaglobin均阳性,11例SOX-10阳性,10例GATA-3阳性,14例DOG1均阴性,Ki-67增殖指数为1%~15%。FISH检测:12例SCSG成功检测到FISH信号,10例发现ETV6-NTRK3基因融合阳性。14例SCSG均行Pan-TRK免疫组化检测,10例细胞核阳性,1例细胞质阳性。10例腺泡细胞癌、10例黏液表皮样癌、10例腺样囊性癌中分别有2、4、7例Pan-TRK阳性,定位于细胞膜/细胞质。结论Pan-TRK免疫组化阳性可提示伴ETV6-NTRK3融合的SCSG,尤其以细胞核着色模式为阳性标准时,在SCSG诊断及鉴别诊断中表现为较高的敏感性和高度特异性,并对未来TRK基因靶向治疗提供依据。展开更多
Trace element(TE) and rare earth element(REE) contents in red soils from the Dongting Lake area of China were determined to understanding the provenance and weathering characteristics of the red soils.The results show...Trace element(TE) and rare earth element(REE) contents in red soils from the Dongting Lake area of China were determined to understanding the provenance and weathering characteristics of the red soils.The results showed similar REE distribution patterns among red soils from the Dongting Lake area,Xiashu loess from Zhenjiang,loess and Pliocene red soil from the Loess Plateau.These patterns implied a similar provenance from dust-storms,except for red soil R5 which formed by bedrock weathering and had significant light REE(LREE) enrichment and heavy REE(HREE) depletion due to longer weathering periods and higher intensity of weathering.Trace element,especially the Rb/Sr and Li/Ba ratios,and REE,especially the LREE/HREE ratio and δEu could trace weathering intensity.Higher Rb/Sr,Li/Ba,and LREE/HREE ratios and negative Eu anomalies were present in the red soils from the Dongting Lake area.The weathering intensity was in the decreasing order of R5 in the Dongting Lake area > red soils from the Dongting Lake area(including reticulate red soil,weak reticulate red soil,and homogeneous red soil formed by dust storms) > Xiashu loess from Zhenjiang > loess-paleosol and Pliocene red soil from the Loess Plateau.Variations in the TE and REE contents of soil could be effectively used to study the provenance and the weathering intensity.展开更多
The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at differen...The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at different dosages before ethanol administration. Transaminases, glutathione S-transferase A1 level and hepatic biochemical indices(malondialdehyde, superoxide dismutase, glutathione and glutathione peroxidase) were determined. Pretreatment with FS extracts significantly inhibited the damage caused by ethanol and the hepatoprotective effects of FS were almost similar to Silymarin that was used to treat alcoholic liver injury. GSTA1 contents in all the FS extract-treated groups were significantly different from those in the ethanol-induced acute liver injury model group(p<0.01), and similar trends were observed in transaminases and hepatic indices level both in vitro and in vivo. The results showed that FS extracts had hepatoprotective effects against ethanol-induced injury. Those effects might be related to the enhancement of antioxidant capacity of liver cells, and FS extracts could reduce the release of liver GSTA1, which contributed to improve liver detoxification.展开更多
文摘目的探讨Pan-TRK免疫组化在涎腺分泌性癌(secretory carcinoma of salivary glands,SCSG)诊断及鉴别诊断中的价值。方法收集14例SCSG临床资料,采用免疫组化、FISH对SCSG进行检测。另选取经典的腺泡细胞癌、黏液表皮样癌、腺样囊性癌各10例作为对照。结果14例SCSG患者男女比为4∶3,年龄15~79岁,10例发生于腮腺,2例发生于颊部,1例发生于腭部,1例发生于颈部;11例生存状态良好,1例复发生存,2例失访。14例SCSG,多呈浸润性生长,呈微囊状、乳头-囊状、管状、实性排列或几者混合,腔内伴丰富分泌物,1例见神经侵犯,1例伴淋巴结转移。免疫表型:14例SCSG中CK7、S-100、Mammaglobin均阳性,11例SOX-10阳性,10例GATA-3阳性,14例DOG1均阴性,Ki-67增殖指数为1%~15%。FISH检测:12例SCSG成功检测到FISH信号,10例发现ETV6-NTRK3基因融合阳性。14例SCSG均行Pan-TRK免疫组化检测,10例细胞核阳性,1例细胞质阳性。10例腺泡细胞癌、10例黏液表皮样癌、10例腺样囊性癌中分别有2、4、7例Pan-TRK阳性,定位于细胞膜/细胞质。结论Pan-TRK免疫组化阳性可提示伴ETV6-NTRK3融合的SCSG,尤其以细胞核着色模式为阳性标准时,在SCSG诊断及鉴别诊断中表现为较高的敏感性和高度特异性,并对未来TRK基因靶向治疗提供依据。
基金supported by the Science Foundation of Nanjing University of Information Science and Technology,China(No.20080328)the National Key Technology R&D Program (No.2006BAK21B02)+1 种基金the National Key Basic Research Program (973 Program) of China (No.2003CB415201)the National Natural Science Foundation of China (No.40671016)
文摘Trace element(TE) and rare earth element(REE) contents in red soils from the Dongting Lake area of China were determined to understanding the provenance and weathering characteristics of the red soils.The results showed similar REE distribution patterns among red soils from the Dongting Lake area,Xiashu loess from Zhenjiang,loess and Pliocene red soil from the Loess Plateau.These patterns implied a similar provenance from dust-storms,except for red soil R5 which formed by bedrock weathering and had significant light REE(LREE) enrichment and heavy REE(HREE) depletion due to longer weathering periods and higher intensity of weathering.Trace element,especially the Rb/Sr and Li/Ba ratios,and REE,especially the LREE/HREE ratio and δEu could trace weathering intensity.Higher Rb/Sr,Li/Ba,and LREE/HREE ratios and negative Eu anomalies were present in the red soils from the Dongting Lake area.The weathering intensity was in the decreasing order of R5 in the Dongting Lake area > red soils from the Dongting Lake area(including reticulate red soil,weak reticulate red soil,and homogeneous red soil formed by dust storms) > Xiashu loess from Zhenjiang > loess-paleosol and Pliocene red soil from the Loess Plateau.Variations in the TE and REE contents of soil could be effectively used to study the provenance and the weathering intensity.
基金Supported by the National Natural Science Foundation of China(31472241)the Application Technology Research and Development Projects in Heilongjiang Province of China(PC13S03)
文摘The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at different dosages before ethanol administration. Transaminases, glutathione S-transferase A1 level and hepatic biochemical indices(malondialdehyde, superoxide dismutase, glutathione and glutathione peroxidase) were determined. Pretreatment with FS extracts significantly inhibited the damage caused by ethanol and the hepatoprotective effects of FS were almost similar to Silymarin that was used to treat alcoholic liver injury. GSTA1 contents in all the FS extract-treated groups were significantly different from those in the ethanol-induced acute liver injury model group(p<0.01), and similar trends were observed in transaminases and hepatic indices level both in vitro and in vivo. The results showed that FS extracts had hepatoprotective effects against ethanol-induced injury. Those effects might be related to the enhancement of antioxidant capacity of liver cells, and FS extracts could reduce the release of liver GSTA1, which contributed to improve liver detoxification.
