The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the ident...The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines （RILs） into two sub-populations： one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two （qFT-B1 and qFT-H） in RILs with the E1 allele and one （qFT-J-2） in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.展开更多
目的探究人参皂苷Rg_(1)对顺铂损伤大鼠卵巢颗粒细胞的保护作用及分子机制。方法选取22~24 d SD的大鼠提取卵巢颗粒细胞进行原代培养,应用顺铂诱导建立卵巢早衰模型。设置正常组,模型组,人参皂苷Rg_(1)低浓度组、中浓度组、高浓度组和...目的探究人参皂苷Rg_(1)对顺铂损伤大鼠卵巢颗粒细胞的保护作用及分子机制。方法选取22~24 d SD的大鼠提取卵巢颗粒细胞进行原代培养,应用顺铂诱导建立卵巢早衰模型。设置正常组,模型组,人参皂苷Rg_(1)低浓度组、中浓度组、高浓度组和雌二醇(E2)组。HE染色及免疫细胞化学法进行颗粒细胞鉴定;CCK8法检测人参皂苷Rg_(1)作用24 h、48 h对顺铂损伤颗粒细胞的保护作用;Hoechst 33258染色检测细胞凋亡;Western blot法检测FSHR、PI3K、p-AKT、AKT、Bcl-2和Bax的蛋白表达。结果细胞形态观察和胞质FSHR表达检测结果均表明所提取细胞为卵巢颗粒细胞;顺铂处理12 h后,颗粒细胞增殖率呈剂量依赖性下降,而给予人参皂苷Rg_(1)后,细胞增殖率下降被显著抑制,且呈剂量和时间依赖性;与模型组比较,人参皂苷Rg_(1)和E2处理后卵巢颗粒细胞中FSHR、PI3K、p-AKT/AKT及Bcl-2/Bax蛋白表达水平显著上升,而PI3K抑制剂干预后,Bcl-2蛋白表达显著下降。结论人参皂苷Rg_(1)对顺铂损伤的卵巢颗粒细胞具有保护作用,该保护作用可能通过激活FSHR/PI3K/AKT通路,抑制卵巢颗粒细胞凋亡实现。展开更多
基金partially supported by the National Natural Science Foundation of China (31430065, 31571686, 31201222 and 31371643)the Open Foundation of the Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences+5 种基金the “Hundred Talents” Program of the Chinese Academy of Sciencesthe Strategic Action Plan for Science and Technology Innovation of the Chinese Academy of Sciences (XDA08030108)the Natural Science Foundation of Heilongjiang Province, China (ZD201001, JC201313)the Research and Development of Applied Technology Project, Harbin, China (2014RFQYJ055)the Scientific Research Foundation for Returned Chinese Scholars of Heilongjiang Province, China (LC201417)the Science Foundation for Creative Research Talents of Harbin Science and Technology Bureau, China (2014RFQYJ046)
文摘The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines （RILs） into two sub-populations： one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two （qFT-B1 and qFT-H） in RILs with the E1 allele and one （qFT-J-2） in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.
