目的评估不同生物材料[矿物三氧化物凝聚体(mineral trioxide aggragate,MTA)和iRoot BP Plus]在因龋露髓成熟恒牙活髓保存治疗中的临床效果。方法选取2020年10月至2023年5月于呼和浩特市口腔医院牙体牙髓科就诊的因龋露髓患者200例(20...目的评估不同生物材料[矿物三氧化物凝聚体(mineral trioxide aggragate,MTA)和iRoot BP Plus]在因龋露髓成熟恒牙活髓保存治疗中的临床效果。方法选取2020年10月至2023年5月于呼和浩特市口腔医院牙体牙髓科就诊的因龋露髓患者200例(200颗患牙),采用随机数字表法将患者分为MTA组(MTA为盖髓剂)和iRoot BP Plus组(iRoot BP Plus为盖髓剂),每组各100例(100颗患牙)。比较2组的临床疗效,牙本质桥、牙齿变色、髓腔钙化及牙根吸收发生情况,疼痛视觉模拟量表(visual analogue scale,VAS)评分及满意度差异。结果术后12个月,iRoot BP Plus组治疗成功率为87%(87/100),明显高于MTA组[72%(72/100)],差异有统计学意义(χ^(2)=6.903,P=0.014)。术后3、6、12个月,MTA组牙本质桥发生率分别为5%(5/100)、36%(36/100)、45%(45/100),iRoot BP Plus组分别为8%(8/100)、42%(42/100)、53%(53/100),差异均无统计学意义(均P>0.05);MTA组牙齿变色发生率分别为16%(16/100)、28%(28/100)、46%(46/100),而iRoot BP Plus组所有患牙均未变色(0%),差异均有统计学意义(均P<0.05)。2组患者术后24 h VAS评分及术后12个月满意度评价比较,差异均无统计学意义(均P>0.05)。结论活髓保存治疗因龋露髓成熟恒牙的临床效果良好。相较于MTA,iRoot BP Plus作为盖髓剂的治疗成功率更高,且不易导致牙齿变色,临床应用前景广阔。展开更多
Background Endometriosis （EM） is a benign gynecologic disease predominantly found in women of reproductive age. However, its pathogenesis is still poorly understood. Our experiment was designed to establish a stable...Background Endometriosis （EM） is a benign gynecologic disease predominantly found in women of reproductive age. However, its pathogenesis is still poorly understood. Our experiment was designed to establish a stable and reliable cultural environment for coculture of endometrium and peritoneum, so as to observe the adhesion/invasion ability of endometrium from patients with or without EM. Methods Endometria of secretory phase and peritoneum were sampled from 6 women with endometriois during laparoscopy. Six with ovarian teratoma or simple ovarian cyst were taken as control. We cocultured endometrium and peritoneum into four groups （endometrium from EM cultured with peritoneum from EM, endometrium from control cultured with peritoneum from control, endometrium from EM cultured with peritoneum from non-EM and the endometrium from control cultured with peritoneum from EM） to observe the adhesion/invasion process in gas-liquid surface culture and in-medium culture. Specimens were collected at 1 hour, 6 hours, 12 hours, 24 hours, 2 days, 3 days, 4 days, 5 days, 6 days and 7 days for histology, immunofluorescence and immunohistochemical analysis on cytokeratin 8 （CK8） and CD10. Results The gas-liquid surface culture was superior to in-medium culture for the maintenance of tissue morphology and survival of endometrium. CK8 immunoflurescence demonstrated no remarkable difference in adhesion process between patients with and without EM. CD10 immunochemistry manifested frequent invasion of endometrial stromal cells from EM patients into peritoneum of up to 3 days culture, while the endometriotic cells from non-EM patients did not invade into peritoneum. Conclusions Gas-liquid surface culture is a suitable model for observing the early events in EM lesion formation. Endometrium from patients with EM showed increased invasion capacity during coculture, which might help to explain the etiology of endometriosis.展开更多
文摘目的评估经皮骨锚式助听器(transcutaneous bone-anchored hearing aid,tBCHD)Baha Attract对传导性或混合性聋患者的助听效果及安全性。方法采用前瞻性自身对照研究方法收集13例行Baha Attract植入术的传导性或混合性聋患者临床资料,其中先天性外中耳畸形8例,慢性化脓性中耳炎或中耳胆脂瘤术后4例,慢性中耳炎1例。术前进行纯音测听、言语识别率测试、颞骨CT等评估,术后1个月开机时测试助听后的声场纯音听阈和言语识别率,同时比较术前、术后3个月及1年的言语、空间和音质听觉量表(the speech,spatial,and qualities of hearing scale,SSQ)和健康效用指数量表3(health utilities index mark 3,HUI 3)。结果术后随访42~49个月,发生皮下血肿1例,植入体周围血清肿2例,均治愈。13例患者术前平均气导听阈为66.6±8.0 dB HL,术后声场下助听听阈为24.0±7.1 dB HL;手术前后安静环境下单音节言语识别率分别为24.2%±20.1%、87.7%±7.3%,双音节言语识别率分别为38.9%±24.1%、92.3%±4.8%,手术前后噪声下言语识别率分别为28.9%±22.0%、85.0%±14.4%,与术前比较均有显著差异。SSQ量表结果显示Baha Attract术后患者在言语识别、声源定位及音质评价3个维度得分均明显提高。HUI 3量表结果示Baha Attract术后患者在听力、言语、情绪、疼痛方面显著改善,总体健康状态评分显著提高。结论Baha Attract是一种安全、有效的人工听觉植入装置,可显著提高传导性或混合性聋患者的听力并改善其生活质量,配戴舒适,术后并发症较少。
文摘目的评估不同生物材料[矿物三氧化物凝聚体(mineral trioxide aggragate,MTA)和iRoot BP Plus]在因龋露髓成熟恒牙活髓保存治疗中的临床效果。方法选取2020年10月至2023年5月于呼和浩特市口腔医院牙体牙髓科就诊的因龋露髓患者200例(200颗患牙),采用随机数字表法将患者分为MTA组(MTA为盖髓剂)和iRoot BP Plus组(iRoot BP Plus为盖髓剂),每组各100例(100颗患牙)。比较2组的临床疗效,牙本质桥、牙齿变色、髓腔钙化及牙根吸收发生情况,疼痛视觉模拟量表(visual analogue scale,VAS)评分及满意度差异。结果术后12个月,iRoot BP Plus组治疗成功率为87%(87/100),明显高于MTA组[72%(72/100)],差异有统计学意义(χ^(2)=6.903,P=0.014)。术后3、6、12个月,MTA组牙本质桥发生率分别为5%(5/100)、36%(36/100)、45%(45/100),iRoot BP Plus组分别为8%(8/100)、42%(42/100)、53%(53/100),差异均无统计学意义(均P>0.05);MTA组牙齿变色发生率分别为16%(16/100)、28%(28/100)、46%(46/100),而iRoot BP Plus组所有患牙均未变色(0%),差异均有统计学意义(均P<0.05)。2组患者术后24 h VAS评分及术后12个月满意度评价比较,差异均无统计学意义(均P>0.05)。结论活髓保存治疗因龋露髓成熟恒牙的临床效果良好。相较于MTA,iRoot BP Plus作为盖髓剂的治疗成功率更高,且不易导致牙齿变色,临床应用前景广阔。
基金This study was supported by a grant from the National Natural Science Foundation of China （No. 30571943）.
文摘Background Endometriosis （EM） is a benign gynecologic disease predominantly found in women of reproductive age. However, its pathogenesis is still poorly understood. Our experiment was designed to establish a stable and reliable cultural environment for coculture of endometrium and peritoneum, so as to observe the adhesion/invasion ability of endometrium from patients with or without EM. Methods Endometria of secretory phase and peritoneum were sampled from 6 women with endometriois during laparoscopy. Six with ovarian teratoma or simple ovarian cyst were taken as control. We cocultured endometrium and peritoneum into four groups （endometrium from EM cultured with peritoneum from EM, endometrium from control cultured with peritoneum from control, endometrium from EM cultured with peritoneum from non-EM and the endometrium from control cultured with peritoneum from EM） to observe the adhesion/invasion process in gas-liquid surface culture and in-medium culture. Specimens were collected at 1 hour, 6 hours, 12 hours, 24 hours, 2 days, 3 days, 4 days, 5 days, 6 days and 7 days for histology, immunofluorescence and immunohistochemical analysis on cytokeratin 8 （CK8） and CD10. Results The gas-liquid surface culture was superior to in-medium culture for the maintenance of tissue morphology and survival of endometrium. CK8 immunoflurescence demonstrated no remarkable difference in adhesion process between patients with and without EM. CD10 immunochemistry manifested frequent invasion of endometrial stromal cells from EM patients into peritoneum of up to 3 days culture, while the endometriotic cells from non-EM patients did not invade into peritoneum. Conclusions Gas-liquid surface culture is a suitable model for observing the early events in EM lesion formation. Endometrium from patients with EM showed increased invasion capacity during coculture, which might help to explain the etiology of endometriosis.
