癌因性疲乏气血两虚证患者聚类与富集探索性研究

目的客观划分癌因性疲乏(CRF)气血两虚证人群,探索其潜在的人群富集特征,为实现人群精准辨识提供思路。方法采用横断面研究,于2019年10月-2022年4月纳入CRF气血两虚证患者611例。采集患者临床症状(是否面色淡白或萎黄、少气懒言、头晕、自汗、心悸)及血常规[白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)、中性粒细胞绝对值(NEUT)],并通过Piper疲乏调查量表(PFS)进行疲乏评分。采用R4.2.0进行数据处理与统计分析,借助K-mediods方法进行聚类分析。结果通过无监督划分聚类分析将目标人群分为气血两虚证气虚偏向、气血两虚证轻症、气血两虚证血虚偏向3类。疲乏程度上,前两类人群疲乏程度相似,血虚偏向人群疲乏程度最重;客观指标上,气虚偏向人群WBC、NEUT、PLT水平最低,血虚偏向人群HGB、RBC水平最低,气血两虚证轻症人群血常规各项指标水平均较高。结论通过聚类分析方法,初步在证候基础上实现人群富集分类,为后续的信息富集及人群精准辨识提供了思路。同时贫血指标和WBC、NEUT分别对血虚偏向和气虚偏向呈现出一定指向性,或可加入CRF气血两虚证诊断标准,辅助CRF人群的划分。

孔伯华治疗眩晕用药与治则的数据研究

目的运用数据挖掘的方法分析研究清代京城名医孔伯华治疗眩晕的用药与治则。方法收集整理孔伯华相关著作中治疗眩晕的医案。运用Microsoft Excel 2016进行中药频数统计,利用SPSS 21.0软件进行因子分析。结果共筛选出139首方剂,含355味中药。使用频数排名前五味的中药为,眩晕病:旋覆花、石决明、知母、代赭石、黄柏,体现出眩晕多为肝火上逆,通过聚类分析得,眩晕病分为4类,通过因子分析提取出6个公因子。结论孔老在治疗眩晕时:采用降逆,清肝,升清之法.

Halophyte Vegetation Influences Soil Microbial Community of Coastal Salt Marsh

Coastal wetlands are the most productive ecosystems worldwide and can provide important ecosystem services,yet the characteristics of microbial community within these systems remain poorly understood.Microbial community of salt marsh vegetation and the associated soil physio-chemical properties were investigated in this study.Three typical Suaeda australis,Phragmites australis,Spartina alterniflora wetlands,and non-vegetated bare mudflats in the Zhoushan Islands were studied to advance the understanding of the characteristics of soil bacterial communities in coastal wetlands.Results showed that the bare mudflats exhibited high pH value and soil moisture content compared with the vegetated samples.In different vegetation types,the organic matter content,total nitrogen,and total potassium content decreased in the order:S.alterniflora wetland>P.australis wetland>S.australis wetland,and there was no obvious difference in total phosphorous content.The halophytes could decrease soil salinity compared with bare mudflats.Proteobacteria,Nitrospinae,Bacteroidetes,Acidobacteria,and Nitrospirae were the predominant level across all samples.Functional prediction showed that SPA-covered soil might play vital roles in sulphur cycling,while SUA and PHR covered soils were involved in nitrogen cycling.This study could provide the first insight into the microbial community of this study area and contribute to a better understanding of vegetation microbiota and bioremediation in coastal wetland ecosystem.

中医药领域核心指标集研究现状及展望

目的 随着中医药领域核心指标集研究数量的增多,研究中存在的问题也逐渐凸显,甚至可能影响核心指标集的推广和应用。本研究基于研究注册平台和文献数据库检索对中医药领域核心指标集研究现状进行分析,希望能引起相关研究者重视。方法 计算机检索国内外核心指标集研究注册平台及中英文文献数据库中收录的中医药领域核心指标集研究,检索时限从建库至2022年6月,采用定性分析方法对中医药领域核心指标集研究现状进行梳理总结。结果 从核心指标集研究注册平台检索到72项注册的中医药领域核心指标集研究,这些研究存在疾病分布不均衡,中医特色指标关注不足,核心指标集适用范围不清晰,患者及公众参与力度不充分等问题。从文献数据库中检索到99篇中医药领域核心指标集文献,仅7.07%(7/99)篇为研究结果报告,且被临床研究和系统评价引用的较少。结论 未来研究者应从规范中医药领域核心指标集研究的注册,完善核心指标集研究的方法学,拓展核心指标集的应用场景,并加强不同利益相关群体合作等方面出发,以提高中医药领域核心指标集研究水平和应用。