A white rot fungus strain, Trichoderma sp.AH, was isolated from rotten wood in Fushun and used to study the mechanism of lignite bio-solubilization.The results showed that nitric acid pretreated Fushun lignite was sol...A white rot fungus strain, Trichoderma sp.AH, was isolated from rotten wood in Fushun and used to study the mechanism of lignite bio-solubilization.The results showed that nitric acid pretreated Fushun lignite was solubilized by T.sp.AH and that extracellular proteins from T.sp.AH were correlated with the lignite bio-solubilization results.In the presence of Fushun lignite the extracellular protein concentration from T.sp.AH was 4.5 g/L while the concentration was 3 g/L in the absence of Fushun lignite.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) of the extracellular proteins detected at least four new protein bands after the T.sp.AH had solubilized the lignite.Enzyme color reactions showed that extracellular proteins from T.sp.AH mainly consisted of phenol-oxidases, but that lignin decomposition enzymes such as laccase, peroxidase and manganese peroxidases were not present.展开更多
Lignite bio-solubilization is a promising technology for converting solid lignite into oil.This study concerns the adsorption of lignite-solubilizing enzymes onto the lignite surface.Adsorption capacity, infrared spec...Lignite bio-solubilization is a promising technology for converting solid lignite into oil.This study concerns the adsorption of lignite-solubilizing enzymes onto the lignite surface.Adsorption capacity, infrared spectral analysis and driving forces analysis are studied as a way to help understand the bio-solubilization mechanism.The results show that the amount of lignite bio-solubilization is proportional to the amount of adsorbed lignite-solubilizing enzymes.An increase in lignite-solubilizing enzyme adsorption of 10% leads to a 7% increase in lignite bio-solubilization.However, limited amounts of enzymes can be adsorbed by the lignite, thus resulting in low percentages of bio-solubilization.Infrared spectral analysis shows that side chains, such as hy-droxyl and carbonyl, of the lignite structure are the main, and necessary, structures where lignite-solubilizing enzymes attachto the lignite.Furthermore, driving force analysis indicates that the electrostatic force between lignite and enzymes is the main adsorption mechanism.The forces are influenced by solution pH levels, the zeta potential of the lignite and the isoelectric points of the en-zymes.展开更多
The pretreatment of low rank coal with nitric acid oxidation can promote its bio-liquefaction. However, the detailed mechanism of which remains an unresolved problem. In the present work, the characteristics of Fushun...The pretreatment of low rank coal with nitric acid oxidation can promote its bio-liquefaction. However, the detailed mechanism of which remains an unresolved problem. In the present work, the characteristics of Fushun coal before and after oxidation by nitric acid were investigated combined with elemental composition, pore volume and pore size, Zeta potential, FT-IR, and 13C solid NMR spectrum analysis. The results show that the inorganic substance inlaid in coal are dissolved by ni- tric acid, which results in the decrease of coal ash content and increase of pore volume and pore size. Furthermore, there exist obvious chemical reactions between nitric acid and the functional groups of coal including aromatic ring carboxylation, side chain alkyl of aromatic ring oxidation and aromatic ring nitration. Among these reactions, some led to the increase in content of carboxyl, aliphatic carbon connected with O and humic acid carbon, while others caused the reduction of aromaticity, methyl carbon, substituted aryl carbon and side chain.展开更多
Two Cu(Ⅱ) complexes of [Cu(2,6-DPC)(Hnta)(H2O)]·H2O(2,6-DPC = 2,6-pyridinedicarboxylic-carboxylate, Hnta = nicotinic acid) and [Cu2(2,4-D)4(Hnta)2](2,4-D = 2,4-dichlorophenoxyacetic-carboxylate) were synthesized...Two Cu(Ⅱ) complexes of [Cu(2,6-DPC)(Hnta)(H2O)]·H2O(2,6-DPC = 2,6-pyridinedicarboxylic-carboxylate, Hnta = nicotinic acid) and [Cu2(2,4-D)4(Hnta)2](2,4-D = 2,4-dichlorophenoxyacetic-carboxylate) were synthesized and successfully obtained as single crystals in this paper. The supramolecular structures of the complexes from zero to three dimensions and the weak intermolecular force in the crystal were analyzed. The single-crystal structures of the complexes were further analyzed by Hirshfeld surface analysis. The competition capacity of the complexes about calf thymus DNA(ct-DNA) was also analyzed by fluorescence spectroscopy. The ability of complexes to cleave the plasmid DNA(pBR322-DNA) was determined by gel electrophoresis assay. And at last, the complexes’ cytotoxic activities were reviewed for four kinds of cancer cell lines(HeLa, MCF-7, HepG, SKOV-3), showing that the coordination polymer has anti-tumor activity and cytotoxicity.展开更多
基金Projects 50874107 and 50374068 supported by the National Natural Science Foundation of ChinaCPEUKF06-12 by the Foundation of Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education of China
文摘A white rot fungus strain, Trichoderma sp.AH, was isolated from rotten wood in Fushun and used to study the mechanism of lignite bio-solubilization.The results showed that nitric acid pretreated Fushun lignite was solubilized by T.sp.AH and that extracellular proteins from T.sp.AH were correlated with the lignite bio-solubilization results.In the presence of Fushun lignite the extracellular protein concentration from T.sp.AH was 4.5 g/L while the concentration was 3 g/L in the absence of Fushun lignite.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) of the extracellular proteins detected at least four new protein bands after the T.sp.AH had solubilized the lignite.Enzyme color reactions showed that extracellular proteins from T.sp.AH mainly consisted of phenol-oxidases, but that lignin decomposition enzymes such as laccase, peroxidase and manganese peroxidases were not present.
基金Projects 50874107 and 50374068 supported by the National Natural Science Foundation of ChinaCPEUKF06-12 by the Foundation of Key Laboratoryof Coal Processing & Efficient Utilization, Ministry of Education of China
文摘Lignite bio-solubilization is a promising technology for converting solid lignite into oil.This study concerns the adsorption of lignite-solubilizing enzymes onto the lignite surface.Adsorption capacity, infrared spectral analysis and driving forces analysis are studied as a way to help understand the bio-solubilization mechanism.The results show that the amount of lignite bio-solubilization is proportional to the amount of adsorbed lignite-solubilizing enzymes.An increase in lignite-solubilizing enzyme adsorption of 10% leads to a 7% increase in lignite bio-solubilization.However, limited amounts of enzymes can be adsorbed by the lignite, thus resulting in low percentages of bio-solubilization.Infrared spectral analysis shows that side chains, such as hy-droxyl and carbonyl, of the lignite structure are the main, and necessary, structures where lignite-solubilizing enzymes attachto the lignite.Furthermore, driving force analysis indicates that the electrostatic force between lignite and enzymes is the main adsorption mechanism.The forces are influenced by solution pH levels, the zeta potential of the lignite and the isoelectric points of the en-zymes.
基金Supported by the National Natural Science Foundation of China （50874107） the Guizhou Science and Technology Fund （Qiankehe J zi [2012]2306）+1 种基金 the Guizhou High-level Talent Special Assistant Fund （TZJF-2011-04） the Guizhou Research Laboratory Platform of Clean and Efficient Use of Coal Resources （Qianke Platform [2011] 4003）
文摘The pretreatment of low rank coal with nitric acid oxidation can promote its bio-liquefaction. However, the detailed mechanism of which remains an unresolved problem. In the present work, the characteristics of Fushun coal before and after oxidation by nitric acid were investigated combined with elemental composition, pore volume and pore size, Zeta potential, FT-IR, and 13C solid NMR spectrum analysis. The results show that the inorganic substance inlaid in coal are dissolved by ni- tric acid, which results in the decrease of coal ash content and increase of pore volume and pore size. Furthermore, there exist obvious chemical reactions between nitric acid and the functional groups of coal including aromatic ring carboxylation, side chain alkyl of aromatic ring oxidation and aromatic ring nitration. Among these reactions, some led to the increase in content of carboxyl, aliphatic carbon connected with O and humic acid carbon, while others caused the reduction of aromaticity, methyl carbon, substituted aryl carbon and side chain.
基金Supported by the Guizhou Provincial Science and Technology Foundation(No.LKLS[2013]20)the Scientific and Technological Innovation Platform of Liupanshui(52020-2018-02-01 and 52020-2017-02-02)+1 种基金Youth Talent Growth Project of Educational Department of Guizhou Province(No.KY [2017]271)2011 Collaborative Innovation Center of Guizhou Province(Qian Jiao he xietongchuangxin zi [2016]02)
文摘Two Cu(Ⅱ) complexes of [Cu(2,6-DPC)(Hnta)(H2O)]·H2O(2,6-DPC = 2,6-pyridinedicarboxylic-carboxylate, Hnta = nicotinic acid) and [Cu2(2,4-D)4(Hnta)2](2,4-D = 2,4-dichlorophenoxyacetic-carboxylate) were synthesized and successfully obtained as single crystals in this paper. The supramolecular structures of the complexes from zero to three dimensions and the weak intermolecular force in the crystal were analyzed. The single-crystal structures of the complexes were further analyzed by Hirshfeld surface analysis. The competition capacity of the complexes about calf thymus DNA(ct-DNA) was also analyzed by fluorescence spectroscopy. The ability of complexes to cleave the plasmid DNA(pBR322-DNA) was determined by gel electrophoresis assay. And at last, the complexes’ cytotoxic activities were reviewed for four kinds of cancer cell lines(HeLa, MCF-7, HepG, SKOV-3), showing that the coordination polymer has anti-tumor activity and cytotoxicity.
