目的系统评价和整合护理本科生对高仿真模拟(high-fidelity simulation,HFS)教学的体验,为护理教学方法改革提供参考。方法计算机检索PubMed、Cochrane Library、Web of Science、Embase、CINAHL、PsycINFO、中国知网、万方数据库、维...目的系统评价和整合护理本科生对高仿真模拟(high-fidelity simulation,HFS)教学的体验,为护理教学方法改革提供参考。方法计算机检索PubMed、Cochrane Library、Web of Science、Embase、CINAHL、PsycINFO、中国知网、万方数据库、维普数据库、中国生物医学文献服务系统,纳入关于护理本科生对HFS教学体验的质性研究,检索时限为建库至2023年6月。结果纳入9篇研究,提炼出40个研究结果,归纳为7个类别,形成3个整合结果:学习获益和成长体验、角色转换的体验、教学过程中的消极体验。结论护理本科生对HFS教学有着积极的态度和体验,安全、逼真的教学环境和不同角色的学习体验能促进其知识的建构、临床综合能力的提升和护理职业素养的形成,但仍需完善案例运行与教学内容。展开更多
缺氧诱导因子(hypoxia-inducible factor, HIF)与肝细胞癌的发生发展相关。HIF-1α在包括肝细胞癌在内的多种癌症类型的发生发展中发挥着重要作用,但其在肝细胞癌中的靶基因尚未完全确定。为找到HIF-1α在肝癌中新的致癌靶点,通过整合HI...缺氧诱导因子(hypoxia-inducible factor, HIF)与肝细胞癌的发生发展相关。HIF-1α在包括肝细胞癌在内的多种癌症类型的发生发展中发挥着重要作用,但其在肝细胞癌中的靶基因尚未完全确定。为找到HIF-1α在肝癌中新的致癌靶点,通过整合HIF-1α敲除的RNA-seq数据,HIF-1α的ChIP-Seq数据,HIF-1α在肝癌中的共表达基因,以及肝癌相关的GEO(Gene Expression Omnibus)数据集,寻找HIF-1α的潜在靶基因。通过分析TCGA(The Cancer Genome Atlas)肝癌数据库、GEO和HPA(Human Protein Atlas)数据集,研究HIF-1α与ATP2C1的相关性,ATP2C1在肝癌中的表达及预后。通过建立物理和化学(氯化钴)缺氧模型验证ATP2C1与低氧及HIF-1α的关系。通过GO(Gene Ontology),KEGG(Kyoto Encyclopedia of Genes and Genomes)和GSEA(Gene Set Enrichment Analysis)分析探索ATP2C1的生物学功能。通过设计体外实验证实ATP2C1对HCC的作用。利用STRING和BioGRID两个蛋白互作在线数据库获得ATP2C1的互作蛋白,并研究其在肝癌中的表达及相关性。通过整合及筛选数据,ATP2C1被鉴定为一个HIF-1α的潜在靶基因。ATP2C1与HIF-1α高度相关,在肝细胞癌中高表达,且伴随有不良预后。富集分析与体外实验的结果表明ATP2C1参与调控HCC细胞的增殖迁移。蛋白互作数据表明ATP2C1与TMEM165存在互作关系,生存分析表明TMEM1651高表达的肝癌患者预后较差。相关性分析的结果显示ATP2C1与肝癌中TMEM165和MMP2的表达高度相关,表明ATP2C1可能与TMEM165和MMP2存在互作关系,并参与了肝癌的进展过程。结果表明,ATP2C1是HIF-1α的靶基因和肝细胞癌的生物标志物,其敲低抑制了HCC的增殖和迁移。展开更多
Focused on“energy security”,US energy policies have undergone a multi-stage evolution.The main line has shifted from ensuring the oil and gas supply to achieving energy independence,and then to diversified developme...Focused on“energy security”,US energy policies have undergone a multi-stage evolution.The main line has shifted from ensuring the oil and gas supply to achieving energy independence,and then to diversified development under a broader concept of energy security.The development path of US oil and gas companies is guided and influenced by series of policies.Represented by ExxonMobil and Chevron,US oil companies are maintaining a long commitment to the oil and gas business,especially unconventional resources such as shale gas and shale oil.Different from their European counterparts,US oil companies have chosen CCS/CCUS as their primary method for achieving carbon neutrality.As for the new energy business,the US oil giants are showing more interest in raw materials such as lithium resources rather than the power generation business.展开更多
文摘目的系统评价和整合护理本科生对高仿真模拟(high-fidelity simulation,HFS)教学的体验,为护理教学方法改革提供参考。方法计算机检索PubMed、Cochrane Library、Web of Science、Embase、CINAHL、PsycINFO、中国知网、万方数据库、维普数据库、中国生物医学文献服务系统,纳入关于护理本科生对HFS教学体验的质性研究,检索时限为建库至2023年6月。结果纳入9篇研究,提炼出40个研究结果,归纳为7个类别,形成3个整合结果:学习获益和成长体验、角色转换的体验、教学过程中的消极体验。结论护理本科生对HFS教学有着积极的态度和体验,安全、逼真的教学环境和不同角色的学习体验能促进其知识的建构、临床综合能力的提升和护理职业素养的形成,但仍需完善案例运行与教学内容。
文摘缺氧诱导因子(hypoxia-inducible factor, HIF)与肝细胞癌的发生发展相关。HIF-1α在包括肝细胞癌在内的多种癌症类型的发生发展中发挥着重要作用,但其在肝细胞癌中的靶基因尚未完全确定。为找到HIF-1α在肝癌中新的致癌靶点,通过整合HIF-1α敲除的RNA-seq数据,HIF-1α的ChIP-Seq数据,HIF-1α在肝癌中的共表达基因,以及肝癌相关的GEO(Gene Expression Omnibus)数据集,寻找HIF-1α的潜在靶基因。通过分析TCGA(The Cancer Genome Atlas)肝癌数据库、GEO和HPA(Human Protein Atlas)数据集,研究HIF-1α与ATP2C1的相关性,ATP2C1在肝癌中的表达及预后。通过建立物理和化学(氯化钴)缺氧模型验证ATP2C1与低氧及HIF-1α的关系。通过GO(Gene Ontology),KEGG(Kyoto Encyclopedia of Genes and Genomes)和GSEA(Gene Set Enrichment Analysis)分析探索ATP2C1的生物学功能。通过设计体外实验证实ATP2C1对HCC的作用。利用STRING和BioGRID两个蛋白互作在线数据库获得ATP2C1的互作蛋白,并研究其在肝癌中的表达及相关性。通过整合及筛选数据,ATP2C1被鉴定为一个HIF-1α的潜在靶基因。ATP2C1与HIF-1α高度相关,在肝细胞癌中高表达,且伴随有不良预后。富集分析与体外实验的结果表明ATP2C1参与调控HCC细胞的增殖迁移。蛋白互作数据表明ATP2C1与TMEM165存在互作关系,生存分析表明TMEM1651高表达的肝癌患者预后较差。相关性分析的结果显示ATP2C1与肝癌中TMEM165和MMP2的表达高度相关,表明ATP2C1可能与TMEM165和MMP2存在互作关系,并参与了肝癌的进展过程。结果表明,ATP2C1是HIF-1α的靶基因和肝细胞癌的生物标志物,其敲低抑制了HCC的增殖和迁移。
文摘Focused on“energy security”,US energy policies have undergone a multi-stage evolution.The main line has shifted from ensuring the oil and gas supply to achieving energy independence,and then to diversified development under a broader concept of energy security.The development path of US oil and gas companies is guided and influenced by series of policies.Represented by ExxonMobil and Chevron,US oil companies are maintaining a long commitment to the oil and gas business,especially unconventional resources such as shale gas and shale oil.Different from their European counterparts,US oil companies have chosen CCS/CCUS as their primary method for achieving carbon neutrality.As for the new energy business,the US oil giants are showing more interest in raw materials such as lithium resources rather than the power generation business.
