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动物生物安全四级实验室中半开放式猴负压隔离器的性能检测与现存问题思考 被引量:1
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作者 王栋 张兆军 +6 位作者 陈爱宇 张珂智 佟海龙 孙百明 石星明 常宗涌 鲍昀利 《洁净与空调技术》 2020年第4期115-119,共5页
目的:通过对半开放式猴负压隔离器的性能检测与现存问题的探讨,掌握其性能现状,以确保动物生物安全四级实验室的防护水平。方法:根据GB 14925-2010《实验动物环境及设施》及RB199-2015《实验室设备生物安全性能评价技术规范》,对半开放... 目的:通过对半开放式猴负压隔离器的性能检测与现存问题的探讨,掌握其性能现状,以确保动物生物安全四级实验室的防护水平。方法:根据GB 14925-2010《实验动物环境及设施》及RB199-2015《实验室设备生物安全性能评价技术规范》,对半开放式猴负压隔离器的洁净度、换气次数、动物照度、氨浓度、噪声、工作窗口气流流向、过滤器检漏、压差等进行性能检测。对常见问题进行了分析并提出合理化建议。结果:4个检测项目全部符合技术要求,性能符合国家标准。对照度、风机、一体式拼接尺寸及笼具清洗等问题提出建议及改造方案。结论:确保半开放式猴负压隔离器处于良好的运转状态,并对我国生物安全实验室半开放式猴负压隔离器建造及改造提出了借鉴和指导,为我国发布半开放式猴负压隔离器相关标准的实施提供参考依据。 展开更多
关键词 动物生物安全四级实验室 猴负压隔离器 性能检测 现存问题
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Advances in Antimicrobial Peptides and Expression Strategy 被引量:2
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作者 SUN Yan WANG Yunfeng +5 位作者 CHEN Hongyan shi xingming WANG Mei HUANG Tingting SUN Peng YANG Guihua 《Journal of Northeast Agricultural University(English Edition)》 CAS 2011年第1期85-90,共6页
Antimicrobial peptides are widely distributed in nature,existing in organisms of plants,insects,and vertebrates.It has been approved that antimicrobial peptides have broad spectrum antimicrobial activities,and play a ... Antimicrobial peptides are widely distributed in nature,existing in organisms of plants,insects,and vertebrates.It has been approved that antimicrobial peptides have broad spectrum antimicrobial activities,and play a key modulatory role in the innate immune response and tumor inhibiting activity.Due to the special action mechanism,the antimicrobial peptides become a hot field of genetic engineering.In the present paper,the general properties,mechanism of action,application value,existing problems,the latest progress and the expression strategy were discussed. 展开更多
关键词 antimicrobial peptide molecular biology fusion expression
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NtJAZ1基因敲除对烤烟Y2001烟碱合成和腺毛发育的影响
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作者 侯子航 罗锐 +4 位作者 朱文奇 季盈彤 时兴铭 闫筱筱 崔红 《烟草科技》 CAS CSCD 北大核心 2023年第9期1-9,共9页
烟碱合成和腺毛发育与烟草品质和抗性密切相关,二者受茉莉酸信号的调控。JAZ1是茉莉酸信号途径中关键的负调控基因,为明确NtJAZ1基因敲除对烟碱生物合成、腺毛发育和分泌物积累的影响,采用CRISPR/Cas9技术对烤烟Y2001中的NtJAZ1进行编辑... 烟碱合成和腺毛发育与烟草品质和抗性密切相关,二者受茉莉酸信号的调控。JAZ1是茉莉酸信号途径中关键的负调控基因,为明确NtJAZ1基因敲除对烟碱生物合成、腺毛发育和分泌物积累的影响,采用CRISPR/Cas9技术对烤烟Y2001中的NtJAZ1进行编辑,获得了纯合突变株系HN。与Y2001相比,HN烟苗生长发育无明显差异,叶面腺毛密度降低,但腺头发育完善且比Y2001饱满,腺毛分泌物组分和含量(质量分数)无显著差异。烟苗叶片的烟碱含量提高19.38%,根系烟碱含量提高30.85%,根系中烟碱合成相关基因NtMYC2a、NtMYC2b、NtPMT和NtQPT的表达量极显著增加,烟苗对蚜虫抗性显著增强。NtJAZ1基因敲除虽然对烟腺毛发生有一定影响,但对腺头发育和分泌物合成有明显促进作用,可在不影响叶面分泌物组分和含量的基础上提高烟碱含量和对蚜虫的抗性。因此,NtJAZ1基因是改良烟草品质和抗性的重要靶点之一。 展开更多
关键词 烟草 NtJAZ1 基因编辑 烟碱 腺毛 蚜虫抗性
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Structure, evolution and regional tectonic implications of the Queshan metamorphic core complex in eastern Jiaodong Peninsula of China 被引量:17
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作者 XIA ZengMing LIU JunLai +3 位作者 NI JinLong ZHANG TingTing shi xingming WU Yun 《Science China Earth Sciences》 SCIE EI CAS CSCD 2016年第5期997-1013,共17页
The Queshan MCC is an important example of a crustal extensional structure in the eastern Jiaodong Peninsula along the southeastern margin of the NCC in the Early Cretaceous. The MCC is a typical Cordilleran-type core... The Queshan MCC is an important example of a crustal extensional structure in the eastern Jiaodong Peninsula along the southeastern margin of the NCC in the Early Cretaceous. The MCC is a typical Cordilleran-type core complex with a three-layered structure:(1) the upper plate is constituted by the Cretaceous supradetachment basin and Paleoproterozoic basement;(2) the lower plate comprises the Neoarchean high-grade metamorphic complexes and late Mesozoic granitic intrusions; and(3) the two plates are separated by a master detachment fault. A series of late NEN-oriented brittle faults superimposed on and destructed the early MCC. Petrology, geometry, kinematics, macro- and micro-structures and quartz c-axis fabrics imply that the MCC has a progressive exhumation history from middle-lower to subsurface level(via middle-upper crustal level) under the nearly WNW-ESE regional extensional regime. We present structural and geochronological evidence to constrain the exhumation of the Queshan MCC from ca. 135 to 113 Ma. Based on the comprehensive analysis of the different patterns of extensional structures in the Jiaodong and Liaodong Peninsula, we have defined the Jiao-Liao Early Cretaceou extensional province and further divided the crustal extension of it into two stages: the first stage was the intense flow of the middle-lower crust and the second stage was the extension of the middle-upper crust. Combining the tectonic setting, the lithosphere thinning in the Jiao-Liao Early Cretaceous extensional province can be considered a typical model for the response of crust-mantle detachment faulting under regional extension in East Asia. 展开更多
关键词 Jiaodong 半岛 Queshan 变形核心建筑群 分开差错地区 Jiao 辽河早白垩纪 extensional 诺思中国 Craton
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